Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts

A technology for artificial cultivation and silkworm chrysalis, which is applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of not using silkworm chrysalis to cultivate cicada flowers, etc., and achieves the effects of short cultivation period, simple technical process and high nutritional content.

Active Publication Date: 2016-10-26
JIANGSU UNIV OF SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, up to now, also do not adopt the method that silkworm chrysalis is cultivated cicadae

Method used

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  • Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts
  • Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts
  • Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Isolate from the wild cicadae collected in the bamboo forest of Anji, Zhejiang, and obtain the cicadae strain capable of infecting silkworm chrysalis after multiple generations of screening, inoculate it on PDA solid medium, and culture it in dark at 20°C for 10 days until the mycelium grows Afterwards, alternate light and dark cultivation was carried out at 24° C., the cycle was 14 hours, the light intensity was 350 Lx, the humidity was 95%, and the cultivation time was 10 days. After being covered with spores, wash the spores with 0.03% Tween 80 sterile water to make a cicadae spore suspension and dilute to a concentration of 10 4 individual / mL.

[0028] 2. Disinfect the silkworm chrysalis body surface with 75% alcohol by spraying, inject 5ul of cicadae spore suspension into the subcutaneous tissue of silkworm chrysalis with a micro-syringe, put it into a sterilized petri dish quickly after inoculation, and infect .

[0029] 3. Cultivate the infected silkworm chr...

Embodiment 2

[0032] 1. Isolate from the wild cicadae collected in the bamboo forest of Anji, Zhejiang, and obtain the cicadae strain capable of infecting silkworm chrysalis through multi-generation screening, inoculate it on PDA solid medium, and cultivate it in dark at 21°C for 9 days until the mycelium grows After that, alternate light and dark cultivation was carried out at 23° C., the cycle was 12 hours, the light intensity was 300 Lx, the humidity was 90%, and the cultivation time was 11 days. After being covered with spores, wash the spores with 0.05% Tween 80 sterile water to make a cicadae spore suspension and dilute to a concentration of 10 6 individual / mL.

[0033] 2. Disinfect the body surface of the silkworm chrysalis with 75% alcohol by spraying, put the silkworm chrysalis in the cicada spore suspension and soak for 10 minutes, take it out, put it into a sterilized petri dish, and infect it.

[0034] 3. Cultivate the infected silkworm chrysalis in the dark in an incubator at ...

Embodiment 3

[0037]1. Isolate from the wild cicadae collected in the bamboo forest of Anji, Zhejiang, and obtain the cicadae strain capable of infecting silkworm chrysalis through multi-generation screening, inoculate it on PDA solid medium, and cultivate it in dark at 22°C for 8 days until the mycelium grows Afterwards, alternate light and dark cultivation was carried out at 22° C., with a period of 12 hours, a light intensity of 250 Lx, a humidity of 85%, and a cultivation time of 12 days. After being covered with spores, wash the spores with 0.07% Tween 80 sterile water to make a cicadae spore suspension and dilute to a concentration of 10 7 individual / mL.

[0038] 2. Disinfect the silkworm chrysalis body surface with 75% alcohol by spraying, inject 10ul of cicada spore suspension into the subcutaneous tissue of the silkworm chrysalis with a micro-injector, put it into a sterilized petri dish quickly after inoculation, and infect .

[0039] 3. Cultivate the infected silkworm chrysalis...

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Abstract

The invention discloses a method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts. The method comprises the following steps of separating and screening out cordyceps cicadae bacterial strains from wild cordyceps cicadae, inoculating the screened cordyceps cicadae bacterial strains to culture mediums, performing culturing until mycelium grows out, then performing light-shade alternate culturing, after spores overgrow, picking the spores in a washing manner, and making spore suspension; using in-vivo silkworm chrysalis of domestic silkworms as hosts, and enabling the in-vivo silkworm chrysalis of the domestic silkworms to be infected through cordyceps cicadae spore suspension by a method of injection, soaking or body spraying; and culturing the infected silkworm chrysalis of the domestic silkworms until the silkworm chrysalis is hardened, and then performing light-shade alternate culturing. According to the method disclosed by the invention, the in-vivo silkworm chrysalis is used as the hosts for cultivating the silkworm chrysalis cordyceps cicadae which is high in content of nutrient components and free from toxins, and the silkworm chrysalis cordyceps cicadae can be developed into various foods and health-care products, which are safe and reliable to eat. According to the method disclosed by the invention, the silkworm chrysalis of the domestic silkworms is used for cultivating the cordyceps cicadae, the culturing cycle is short, the technical technology is simple and convenient, and the method is suitable for large-scale production and has wide application prospects.

Description

technical field [0001] The invention belongs to a method for cultivating cicada flowers, in particular to a method for artificially cultivating silkworm chrysalis cicadas with silkworm chrysalis as a host. Background technique [0002] Cicadae (Cordyceps cicadae) is a composite of the subunit and nymph corpse of Cordyceps cicadae Shing parasitizing on the nymphs of Cicadae insects. It is a traditional precious Chinese medicinal material in my country. In China, cicadae has been used as a traditional Chinese medicine and food for more than 1500 years, long before Cordyceps sinensis. Modern medical research shows that cicadae contains a variety of active ingredients, which have the functions of treating convulsions in children, anti-tumor, analgesia and sedation, improving kidney function, anti-fatigue, immune regulation, etc. It is a precious medicinal material similar to Cordyceps sinensis, which can be used as replacement of. [0003] The market demand for cicadae is incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 唐顺明曾光远张有做沈兴家赵珊珊魏兆军赵巧玲刘莉萍刘龙山
Owner JIANGSU UNIV OF SCI & TECH
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