Artificial domestication cultivation method for wild chanterelle
A cultivation method and technology for chanterelles, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problem that the artificial domestication and cultivation of chanterelles has not achieved breakthrough progress, damaged the natural environment of chanterelles, and reduced the output of chanterelles. and other problems, to achieve the effect of enriching the supply of market varieties, shortening the cultivation period and reducing pollution.
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Embodiment 1
[0014] (1) Collection of chanterelles: collected from May to September when the fruiting bodies of chanterelles are mature;
[0015] (3) Separation and cultivation of strains: After cleaning the fruiting bodies of wild chanterelles, take 1000ml of filtrate, add 15g of sucrose, 15g of yeast extract, 1.5g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 20g of agar, adjust the pH of phosphate buffer to 7.5, After making a slant and inoculating it, culture it at a constant temperature of 20°C until the mycelia cover the slant;
[0016] ⑵Original seed culture: Inoculate 10.8g of the mother seed into a test tube for culture based on dark culture at 25°C, and the mycelia can be covered to the bottom of the bottle; the test tube culture medium: 60g of miscellaneous wood chips, 15g of bean stalks (crushed), Gypsum 1g, wheat bran 10g, superphosphate 2g, sucrose 5g, urea 5g, vitamin B0.2g, peptone 10g, set the volume at 1000ml, pH6, sterilize with high pressure steam at 0.1...
Embodiment 2
[0020] A method for artificial domestication and cultivation of wild chanterelles, comprising the steps of:
[0021] (1) Separation and cultivation of strains: collect wild chanterelle fruiting bodies, take 1500ml of filtrate after cleaning, add 20g of sucrose, 20g of yeast extract, 2.0g of potassium dihydrogen phosphate, 1.0g of magnesium sulfate, 25g of agar, adjust the pH to 7 with phosphate buffer , after making a slant and inoculating it, culture it at a constant temperature of 20°C until the mycelia cover the slant;
[0022] ⑵Original seed culture: Inoculate 20.4g of the mother seed into a test tube for culture based on dark culture at 28°C, and the mycelia can be covered to the bottom of the bottle; Gypsum 10g, wheat bran 20g, calcium superphosphate 8g, sucrose 10g, urea 10g, vitamin B1.0g, peptone 15g, set the volume at 1000ml, pH5.0, sterilize with high-pressure steam at 0.15 and 130°C for 30min;
[0023] (3) Cultivation of cultivars: 18.5g of the original seed was i...
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