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Artificial silkworm chrysalis cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts

A technology of artificial cultivation and silkworm chrysalis, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of not using tussah silkworm chrysalis to cultivate cicadas, and achieve the effects of short cultivation period, safe and reliable food, and simple technical process

Active Publication Date: 2016-09-21
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, up to now, also do not adopt the method that tussah silkworm chrysalis is cultivated cicadae

Method used

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  • Artificial silkworm chrysalis cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts
  • Artificial silkworm chrysalis cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts
  • Artificial silkworm chrysalis cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolate from wild cicada flowers collected in the bamboo forest of Anji, Zhejiang. After multiple generations of screening, a cicada flower strain capable of infecting tussah silkworm pupae is obtained, which is inoculated on a PDA solid medium and cultured in the dark at 20°C for 10 days to grow hyphae After incubation, alternate light and dark culture at 24°C, with a period of 14h, light intensity 350Lx, humidity 95%, and a culture time of 10d. After the spores are overgrown, wash the spores with 0.03% Tween 80 sterile water to make a spore suspension of cicada flower and dilute to a concentration of 10 4 Pieces / mL.

[0028] 2. Use 75% alcohol to disinfect the body surface of the tussah silkworm pupa by spraying. Use a micro syringe to inject 10ul of the cicada flower spore suspension into the tussah silkworm pupa subcutaneous tissue. After inoculation, quickly put it into a sterilized petri dish for infection. .

[0029] 3. Culture the infected tussah silkworm pupae i...

Embodiment 2

[0032] 1. Isolate from wild cicada flowers collected in the bamboo forest of Anji, Zhejiang. After multiple generations of screening, a cicada flower strain capable of infecting tussah silkworm pupae is obtained, which is inoculated on PDA solid medium and cultured in the dark at 21°C for 9 days until hyphae grow After incubation, alternate light and dark culture at 23°C, with a cycle of 12h, light intensity of 300Lx, humidity of 90%, and culture time of 11d. After the spores are overgrown, wash the spores with 0.05% Tween 80 sterile water to make a spore suspension of cicada flower and dilute to a concentration of 10 6 Pieces / mL.

[0033] 2. Use 75% alcohol to disinfect the body surface of tussah silkworm chrysalis by spraying, soak the silkworm chrysalis in a suspension of cicada flower spores for 10 minutes, then take it out, and put it into a sterilized petri dish for infection.

[0034] 3. Cultivate the infected tussah silkworm pupa in the dark in an incubator at 22°C until th...

Embodiment 3

[0037] 1. Isolate from wild cicada flowers collected in the bamboo forest of Anji, Zhejiang. After multiple generations of screening, a cicada flower strain capable of infecting tussah silkworm pupae was obtained. Inoculated on PDA solid medium, cultured in dark at 22°C for 8 days until hyphae were grown After incubation, alternate light-dark culture at 22°C, with a period of 12h, light intensity 250Lx, humidity 85%, and culture time 12d. After the spores are overgrown, wash the spores with 0.07% Tween 80 sterile water to make a spore suspension of cicada flower and dilute to a concentration of 10 5 Pieces / mL.

[0038] 2. Use 75% alcohol to disinfect the body surface of tussah silkworm pupa by spraying. Use a micro syringe to inject 15ul of cicada flower spore suspension into the subcutaneous tissue of tussah silkworm pupa. After inoculation, quickly put it into a sterilized petri dish for infection. .

[0039] 3. Culture the infected tussah silkworm pupa in the dark in a 25℃ incu...

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Abstract

The invention discloses an artificial cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts. The method comprises the following steps that cordyceps sobolifera strains are separated and screened from wild cordyceps sobolifera, a culture medium is inoculated with the cordyceps sobolifera strains, culturing is conducted until mycelia grow out, then light and shade alternate culturing is conducted, and after the culture medium is full of spores, the spores are washed down to be prepared into spore suspension; the living tussah silkworm chrysalises are taken as the hosts to be infected by the cordyceps sobolifera spore suspension through an injecting or soaking or body spraying method; the infected tussah silkworm chrysalises are cultured until the silkworm chrysalises become hard, and then light and shade alternate culturing is conducted. According to the method, the silkworm chrysalis cordyceps sobolifera is cultivated by adopting the living silkworm chrysalises as the hosts, rich in nutrition ingredient and free of toxicity, can be developed into various food and healthcare products and can achieve edible safety and reliability. The method for cultivating the cordyceps sobolifera through the tussah silkworm chrysalises has the advantages that the culture cycle is short, the technology is simple and convenient, the method is suitable for large-scale production, and the wide application prospect is achieved.

Description

Technical field [0001] The invention belongs to a method for cultivating cicada flowers, and specifically relates to a method for artificially cultivating silkworm pupa cicada flowers with tussah silkworm pupa as a host. Background technique [0002] Cicada flower (Cordyceps cicadae) is a complex of the sub-base and nymph corpse of Cordyceps cicadae Shing parasitic on the nymphs of the cicada family insects, and it is a kind of precious Chinese traditional medicine in my country. In China, cicada flower has been used as a traditional Chinese medicinal material and food for more than 1,500 years, much earlier than Cordyceps sinensis. Modern medical research shows that cicada flower contains a variety of active ingredients. It has the functions of treating infantile convulsions, anti-tumor, analgesia and sedation, improving kidney function, anti-fatigue, and immune regulation. It is a precious medicinal material similar to Cordyceps sinensis and can be used as Cordyceps sinensis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 唐顺明沈兴家魏兆军曾光远刘莉萍张有做赵巧玲赵珊珊张伟
Owner JIANGSU UNIV OF SCI & TECH
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