Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm

An exogenous additive and protocorm differentiation technology, applied in the biological field, can solve the problems of harsh growth environment, endangered wild resources of clematis, and difficult artificial cultivation, etc., and achieve the effect of protecting wild resources

Inactive Publication Date: 2016-07-06
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, due to unreasonable and indiscriminate mining and harsh requirements on the growth environment, the wild resources of clematis are on the verge of extinction, artificial cultivation is difficult, and the contradiction between supply and demand in the market is prominent

Method used

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  • Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm
  • Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm
  • Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 2 cm of the stem section of Aurantia clematis with joints, soak it in washing powder solution for 6 minutes, wash it with running water for 2 hours, disinfect it with 75% alcohol on a sterile ultra-clean table for 20 seconds, wash it with sterile water for 3 times, and then wash it in 0.1% mercury chloride solution. Sterilize for 8 minutes, wash 6 times with sterile water, cut off both ends of the sterilized stem segment with a scalpel, and inoculate it in MS medium containing 2.0mg / L of 6-BA and 0.2mg / L of NAA , the culture temperature is 25±1°C, the light is 15001x, the light time is 12h / d, and the air humidity is kept at 50%-60%. After 30 days, protocorms grow at both ends of the stem segment.

[0017] After the obtained clematis protocorms were subcultured in the above medium and grown for 30 days, they were cut into 2cm 2 size, inoculated into the differentiation medium, and observed the differentiation phenomenon after 45 days. The composition of medium A is...

Embodiment 2

[0023] Take 2 cm of the stem section of Aurantia clematis with joints, soak it in washing powder solution for 6 minutes, wash it with running water for 2 hours, disinfect it with 75% alcohol on a sterile ultra-clean table for 20 seconds, wash it with sterile water for 3 times, and then wash it in 0.1% mercury chloride solution. Sterilize for 8 minutes, wash 6 times with sterile water, cut off both ends of the sterilized stem segment with a scalpel, and inoculate it in MS medium containing 2.0mg / L of 6-BA and 0.2mg / L of NAA , the culture temperature is 25+1°C, the light is 1500lx, the light time is 12h / d, and the air humidity is kept at 50%-60%. After 30 days, protocorms grow at both ends of the stem segment.

[0024] After the obtained clematis protocorms were subcultured in the above medium and grown for 30 days, they were cut into 2cm 2 size, inoculated into the differentiation medium, and observed the differentiation phenomenon after 45 days. The composition of medium A is...

Embodiment 3

[0030] Take 2 cm of the stem section of Aurantia clematis with joints, soak it in washing powder solution for 6 minutes, wash it with running water for 2 hours, disinfect it with 75% alcohol on a sterile ultra-clean table for 20 seconds, wash it with sterile water for 3 times, and then wash it in 0.1% mercury chloride solution. Sterilize for 8 minutes, wash 6 times with sterile water, cut off both ends of the sterilized stem segment with a scalpel, and inoculate it in MS medium containing 2.0mg / L of 6-BA and 0.2mg / L of NAA , the culture temperature is 25±1°C, the light is 1500lx, the light time is 12h / d, and the air humidity is kept at 50%-60%. After 30 days, protocorms grow at both ends of the stem segment.

[0031] After the obtained clematis protocorms were subcultured in the above medium and grown for 30 days, they were cut into 2cm 2 size, inoculated into the differentiation medium, and observed the differentiation phenomenon after 45 days. The composition of medium A is...

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Abstract

The invention discloses an exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm. The culture medium is composed of a basic culture medium body and an exogenous additive, and by means of the culture medium, differentiation of the anoectochilus formosanus protocorm into seedlings can be obviously promoted. By means of the exogenous additive culture medium, tissue culture seedlings can be provided for large-scale planting of anoectochilus formosanus, and it is beneficial to protect anoectochilus formosanus wild resources.

Description

Technical field: [0001] The invention belongs to the field of biology, and in particular relates to an exogenous additive culture medium for promoting the differentiation of protocorms of clematis. Background technique: [0002] Anoectochilus roxburghii (Wall.) Lindl), also known as golden clematis and golden silk grass, is a perennial herb of the genus Orchidaceae. Known as the king of medicine, golden grass, bird ginseng, etc., it is a precious medicinal material widely used by the people. Its plant type is small and exquisite, the veins are golden yellow, arranged in a net shape, and the leaf shape is beautiful. Mainly distributed in Fujian, Taiwan, Guangdong, Guizhou, Sichuan, Yunnan and other places. It likes shade and humidity, and grows in humid areas of evergreen broad-leaved forests at an altitude of 300-1200 meters, with a temperature of 20-25°C and avoid direct sunlight. Jinlianxian uses the whole herb as medicine, which has the functions of clearing away heat a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 余伯阳张剑钱恩仁
Owner CHINA PHARM UNIV
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