Cordyceps cicadae polysaccharide, preparation method and application thereof in preparation of medicament for preventing renal interstitial fibrosis

A technology of renal interstitial fibrosis and Cordyceps cicadae, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of in-depth research on Cordyceps cicadae, achieve good research value and commercial value, and improve antioxidant capacity , the effect of simple steps

Active Publication Date: 2019-08-30
ANHUI UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the research on Cordyceps cicadae is not deep at home and abroad, and there are fewer varieties o...

Method used

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  • Cordyceps cicadae polysaccharide, preparation method and application thereof in preparation of medicament for preventing renal interstitial fibrosis
  • Cordyceps cicadae polysaccharide, preparation method and application thereof in preparation of medicament for preventing renal interstitial fibrosis
  • Cordyceps cicadae polysaccharide, preparation method and application thereof in preparation of medicament for preventing renal interstitial fibrosis

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Embodiment 1

[0028] One, the present embodiment first prepares the polysaccharide of Cicadae sinensis, and the specific process is as follows:

[0029] (1) Pulverizing the fruiting body of Cordyceps cicadae, passing through a 60-mesh sieve to obtain the fruiting body powder of Cordyceps cicadae;

[0030] (2) Add distilled water at a ratio of 1:10 for ultrasonic extraction for 20 minutes, then place in a water bath at 80°C for 2 hours, filter through four layers of gauze, collect the supernatant, repeat 3 times, and combine the supernatant;

[0031] (3) The supernatant is subjected to ultra-high pressure treatment, the pressure is 400MPa, the ultra-high pressure treatment time is 12min, and the temperature is kept at 20°C;

[0032] (4) Concentrate the treated supernatant to 150ml by rotary evaporation, then add 600ml of absolute ethanol, let it stand at 4°C for 12h, centrifuge at 5000rpm / min to collect the precipitate, add water to dissolve the precipitate to obtain the crude polysaccharide...

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Abstract

The invention discloses a cordyceps cicadae polysaccharide, a preparation method and application thereof in preparation of a medicament for preventing renal interstitial fibrosis. The method comprisesthe following steps: pulverizing cordyceps cicadae fruiting bodies, and performing sieving to obtain cordyceps cicadae fruiting body powder; ultrasonically extracting the fruiting body powder with distilled water, and then carrying out ultrahigh pressure treatment; preparing crude polysaccharide of the cordyceps cicadae fruiting body after the treatment; and then performing separation by using DEAE-cellulose anion column chromatography, and performing freeze-drying to obtain the purified polysaccharide. The method successfully prepares the cicada cordyceps polysaccharide treated and modifiedby ultrahigh pressure, and has the advantages of simple steps and higher efficiency. The intervention on a rat glomerular epithelial cell model shows that the polysaccharide can effectively inhibit the proliferation of rat glomerular epithelial cells (HMC), and has wide application prospect in the aspect of preventing renal interstitial fibrosis. The oxidation resistance of the cordyceps cicadae polysaccharide is obviously improved, and the cordyceps cicadae polysaccharide induces the apoptosis of high-glucose rat glomerular mesangial cells, has no toxicity to normal cells, and has good research value and commercial value.

Description

technical field [0001] The present invention relates to a preparation method and application of an entomogenous fungus, in particular to a kind of polysaccharide of Cicadae sinensis, preparation method and application in preparation of medicine for preventing renal interstitial fibrosis. Background technique [0002] Cordyceps cicadae (Cordyceps cicadae), also known as golden cicada, is a dry complex parasitic on cicada nymphs by Paecilomyces cicadae, a fungus of the genus Cordyceps ergotaceae. one. The recorded history of Cordyceps sinensis is 800 years earlier than Cordyceps sinensis, and some active ingredients are higher than Cordyceps sinensis. Modern medical research shows that Cordyceps cicadae contains a large amount of functional components such as cordycepic acid, cordyceps polysaccharide, adenosine, a variety of alkaloids and ergosterol, which can improve kidney function, relieve chronic renal failure, regulate lipid metabolism, relieve fever and analgesia, Seda...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P13/12C08B37/00A61K36/068
CPCA61K31/715A61P13/12C08B37/0003A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61K2236/55A61K36/068
Inventor 陈蕾杨军岭陈梅钟建平陈彦王亚蒋云董会彬
Owner ANHUI UNIVERSITY
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