Method for artificially culturing Isaria cicadae Miquel
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A technology of artificial cultivation and cicada flower, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems in the field of artificial cultivation of insect-borne fungi that have not yet been seen in the application of deuterium-depleted water
Active Publication Date: 2017-12-01
ZHEJIANG BIOASIA PHARMA CO LTD
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[0005] Most of the existing technologies focus on studies related to tumor suppression and immune regulation with deuterium-d...
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[0037] Seed tank (fermentation tank) cultivation stage: rotation speed: 150r / min, 1.5vvm, temperature: 25°C, time: cultivation for 24-36h.
[0038] Solid medium stage: inoculum ...
Embodiment 2
[0040] Basically the same as Example 1, the only difference is that the water for cultivation is deuterium-depleted water, and the content of deuterium is 50ppm.
Embodiment 3
[0042] It is basically the same as Example 1, except that the water for cultivation is deuterium-depleted water, and the content of deuterium is 100ppm.
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Abstract
The invention relates to a method for artificially culturing Isaria cicadae Miquel. The method comprises the following steps: 1. carrying out amplification culture on an Isaria cicadae Miquel strain; 2. carrying out solid culture on the strain obtained by amplification culture in the step 1; and 3. harvesting sporophores. The method is characterized in that deuterium-depleted water is used in the processes of the amplification culture in the step 1 and solid culture in the step 2, and the content of deuterium does not exceed 100ppm. Due to the adoption of the deuterium-depleted water, the yield and quality of the sporophores of the Isaria cicadae Miquel can be remarkably improved.
Description
technical field [0001] The invention relates to a method for artificially cultivating cicadae. Background technique [0002] Cicada (Isaria cicadae Miquel) belongs to Ascomycota, Pezizomycotina, Sordariomycetes, Hypocreales, Cordycipitaceae of the kingdom Fungi (FUNGI). , Isaria (Isaria), is my country's famous and precious Chinese medicinal materials, is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. [0003] Cicada has unique and extensive effects, such as improving kidney function, anti-tumor, regulating immune system, nourishing and strengthening, improving cell ability, anti-fatigue, lowering ...
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