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Method for artificially culturing Isaria cicadae Miquel

A technology of artificial cultivation and cicada flower, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems in the field of artificial cultivation of insect-borne fungi that have not yet been seen in the application of deuterium-depleted water

Active Publication Date: 2017-12-01
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the existing technologies focus on studies related to tumor suppression and immune regulation with deuterium-depleted water, but there is no application of deuterium-depleted water in the field of artificial cultivation of entomogenous fungi.

Method used

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  • Method for artificially culturing Isaria cicadae Miquel
  • Method for artificially culturing Isaria cicadae Miquel
  • Method for artificially culturing Isaria cicadae Miquel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Training process

[0028] Incline cultivation → shake flask cultivation → seed tank cultivation → fermenter cultivation → solid cultivation;

[0029] 2. Medium

[0030] Slant culture medium: PDA medium (potato 200g, glucose 20g, agar 15-20g, deuterium-depleted water 20ppm to make up 1L, pH natural).

[0031] Liquid medium: YSPS medium (yeast extract 5g, soybean protein isolate 10g, white sugar 35g, deuterium-depleted water 20ppm to make up 1L);

[0032] Solid medium: mix wheat and deuterium-depleted water (20ppm) in a ratio of 1:1.5;

[0033] The medium should be sterilized to ensure sterility;

[0034] 3. Culture conditions

[0035] Slope culture stage: temperature 22°C, time: about 7 days;

[0036] Shake flask culture stage: speed: 150r / min, temperature: 25°C, time: culture 55-60h;

[0037] Seed tank (fermentation tank) cultivation stage: rotation speed: 150r / min, 1.5vvm, temperature: 25°C, time: cultivation for 24-36h.

[0038] Solid medium stage: inoculum ...

Embodiment 2

[0040] Basically the same as Example 1, the only difference is that the water for cultivation is deuterium-depleted water, and the content of deuterium is 50ppm.

Embodiment 3

[0042] It is basically the same as Example 1, except that the water for cultivation is deuterium-depleted water, and the content of deuterium is 100ppm.

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Abstract

The invention relates to a method for artificially culturing Isaria cicadae Miquel. The method comprises the following steps: 1. carrying out amplification culture on an Isaria cicadae Miquel strain; 2. carrying out solid culture on the strain obtained by amplification culture in the step 1; and 3. harvesting sporophores. The method is characterized in that deuterium-depleted water is used in the processes of the amplification culture in the step 1 and solid culture in the step 2, and the content of deuterium does not exceed 100ppm. Due to the adoption of the deuterium-depleted water, the yield and quality of the sporophores of the Isaria cicadae Miquel can be remarkably improved.

Description

technical field [0001] The invention relates to a method for artificially cultivating cicadae. Background technique [0002] Cicada (Isaria cicadae Miquel) belongs to Ascomycota, Pezizomycotina, Sordariomycetes, Hypocreales, Cordycipitaceae of the kingdom Fungi (FUNGI). , Isaria (Isaria), is my country's famous and precious Chinese medicinal materials, is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. [0003] Cicada has unique and extensive effects, such as improving kidney function, anti-tumor, regulating immune system, nourishing and strengthening, improving cell ability, anti-fatigue, lowering ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/20A01G18/40A01G18/50A01G18/70
Inventor 胡国佐张忠亮王玉芹董建飞王莹刘艳菊尹彬陈桃宝徐煜孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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