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Method for artificial cultivation of Isaria cicadae and cordyceps cicadae traditional Chinese medicinal material

A technology of I. cicadae and Chinese medicinal materials, which is applied in the field of artificial cultivation of wild Chinese cicadae species I. cicadae and Chinese medicinal material Cicadae. It can solve problems such as the shortage of wild medicinal materials, and achieve simple cultivation and harvesting conditions, The effect of being less likely to be infected with miscellaneous bacteria and high spore production ability

Active Publication Date: 2018-05-18
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the invention can form spore bundles consistent with the external shape and organizational structure of wild cicadas, effectively solving the problem of shortage of wild medicinal materials

Method used

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  • Method for artificial cultivation of Isaria cicadae and cordyceps cicadae traditional Chinese medicinal material
  • Method for artificial cultivation of Isaria cicadae and cordyceps cicadae traditional Chinese medicinal material
  • Method for artificial cultivation of Isaria cicadae and cordyceps cicadae traditional Chinese medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] ①Preparation of PDA solid slant: The proportion of PDA medium is per 1000mL medium: 200g of potatoes, 20g of glucose, 20g of agar, put the peeled and diced potatoes in a container, add 1000mL of water, boil for 20min, filter, add glucose and agar (liquid culture Add water to 1000mL, heat to dissolve, then divide into test tubes, the filling volume is 1 / 4 of the test tube length, plug with cotton plugs, and sterilize in a sterilizing pot under 0.1MPa pressure for 30min , Place the test tubes on a slant plate while they are hot to cool.

[0029] ②Preparation of slant seed: Take the freeze-dried patented preserved strain, activate it in PDA liquid medium by aseptic operation, transfer the tube, culture it at 22°C for 3 days, and select a good growing strain for use;

[0030] ③PDA Erlenmeyer flask seed solution preparation: per 1000mL of culture solution: 200g of potatoes, 20g of glucose, put the peeled and diced potatoes in the container, add 1000mL of water, boil for 30mi...

Embodiment 2

[0036] ①Preparation of PDA solid slant: The proportion of PDA medium is per 1000mL medium: 200g of potatoes, 20g of glucose, 20g of agar, put the peeled and diced potatoes in a container, add 1000mL of water, boil for 20min, filter, add glucose and agar (liquid culture Add water to 1000mL, heat to dissolve, then divide into test tubes, the filling volume is 1 / 4 of the test tube length, plug with cotton plugs, and sterilize in a sterilizing pot under 0.1MPa pressure for 30min , Place the test tubes on a slant plate while they are hot to cool.

[0037] ②Preparation of slant seed: Take the freeze-dried patented preserved strain, activate it in PDA liquid medium by aseptic operation, transfer the tube, culture it at 22°C for 3 days, and select a good growing strain for use;

[0038] ③PDA Erlenmeyer flask seed solution preparation: per 1000mL of culture solution: 200g of potatoes, 20g of glucose, put the peeled and diced potatoes in the container, add 1000mL of water, boil for 30mi...

Embodiment 3

[0049] ①Preparation of PDA solid slant: The proportion of PDA medium is per 1000mL medium: 200g of potatoes, 10g of glucose, 20g of agar, put the peeled and diced potatoes in a container, add 1000mL of water, boil for 50min, filter, add glucose and agar, and make up water to 1000mL, heated to dissolve, and then dispensed into test tubes, the filling volume is 1 / 4 of the length of the test tube, plugged with a cotton plug, placed in a sterilizer for 30min under a pressure of 0.15MPa, and put the test tube on an inclined Cool on the panel.

[0050] ②Preparation of slant seed: take the freeze-dried patented preserved strain, activate it in PDA liquid medium by aseptic operation, transfer the tube, culture it at 22°C for 5 days, and select a good growing strain for use;

[0051] ③PDA Erlenmeyer flask seed solution preparation: per 1000mL culture solution: 200g of potatoes, 20g of glucose, put the peeled and diced potatoes in the container, add 1000mL of water, boil for 50min, filt...

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Abstract

The invention provides a method for artificial cultivation of Isaria cicadae and a cordyceps cicadae traditional Chinese medicinal material. The Isaria cicadae has a preservation number of CGMCC No.13684, strains of Isaria cicadae are subjected to steps of slant activation culture, seed culture and solid medium cultivation, and a large number of coremia of the cordyceps cicadae traditional Chinesemedicinal material are obtained. The sporulation ability of the Isaria cicadae is extremely strong, the artificial cultivation method is simple and easy, and the coremia as the traditional Chinese medicinal material cordyceps cicadae which is collected in natural forests can be obtained, so that the problem of shortage of a wild cordyceps cicadae medicinal material is solved.

Description

technical field [0001] The invention belongs to the technical field of cultivation of traditional Chinese medicinal materials and bioengineering, and relates to an artificially isolated wild Chinese medicinal cicadae fungus, Issaria cicadae, and a method for artificially cultivating the traditional Chinese medicinal herb cicadae by using the fungus. Background technique [0002] The traditional Chinese medicine cicada is a compound of the ergot fungus Paecilomyces cicadae that parasitizes the nymphs of bamboo cicadas. It is a Cordyceps similar to Cordyceps sinensis. It is cicadae or big cicada, mainly produced in Jiangsu and Zhejiang. my country has a long history of understanding and utilization of cicadae. The earliest record of processing cicadae was recorded in Lei Gong Pao Shao Lun written by Lei Chen in the Northern and Southern Dynasties, 800 years earlier than the record of winter grass and summer grass. Su Song's "Tujing Materia Medica", Song Yaokuan's "Xixi Congyu...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20A01G18/00C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 于瑞莲周玉磊陆宸卫梦婷郭瑞康
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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