Active isaria cicadae miquel substance and application thereof in prevention, delaying or treatment of cataract

An active substance, a technology of cicada flower, applied in the field of cicada flower active substances, prevention, delay or treatment of cataract, can solve the problems of loss of intraocular lens accommodation, unable to perform surgery in time, etc., to improve antioxidant capacity, improve activity, The effect of increasing the content of polysaccharides

Inactive Publication Date: 2019-09-27
兰溪市立顺生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cataract surgery technology has gradually matured, surgical treatment not only brings huge economic problems to patients' families and society, especially for many poor patients in developing countries, mo

Method used

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  • Active isaria cicadae miquel substance and application thereof in prevention, delaying or treatment of cataract
  • Active isaria cicadae miquel substance and application thereof in prevention, delaying or treatment of cataract
  • Active isaria cicadae miquel substance and application thereof in prevention, delaying or treatment of cataract

Examples

Experimental program
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preparation example Construction

[0034] The present invention relates to a kind of preparation method of cicadae active substance, comprising,

[0035]1) Inoculate the Cicadae species activated in the PDA slant medium into the seed liquid medium, at 20-30°C (such as 22°C, 22.5°C, 23°C, 24°C, 27°C, 28°C, etc.) , 100-150r / min (such as 105r / min, 110r / min, 124r / min, 130r / min, 140r / min, 145r / min, etc.) under the shaker for 2-5d (such as 2.5d, 3d, 3.5d, 4d, 4.5d, etc.), to obtain the seed solution;

[0036] 2) inoculate the seed solution of step 1) in the liquid fermentation medium with an inoculum of 5-15% (such as 5.4%, 7%, 8%, 10.8%, 13%, 14.5%, etc.), at 20-30 ℃ (such as 22℃, 22.5℃, 23℃, 24℃, 27℃, 28℃, etc.), 50-100r / min (such as 55r / min, 60r / min, 74r / min, 80r / min, 90r / min, 95r / min, etc.) on a shaking table for 2-5d (such as 2.5d, 3d, 3.5d, 4d, 4.5d, etc.), freeze-dry the mycelium and fermentation broth, crush and pass through a 20-100 mesh sieve to obtain The fermented product of the active substance; the e...

Embodiment 1

[0052] The preparation method of cicadae active substance, comprising,

[0053] 1) Inoculate the Cicada japonica strain activated in the PDA slant medium into the seed liquid medium, and culture it on a shaker at 20° C. and 100 r / min for 2 days to obtain the seed liquid;

[0054] 2) Inoculate the seed solution of step 1) in the liquid fermentation medium with an inoculum amount of 5%, cultivate it on a shaking table at 20° C. and 50 r / min for 2 days, freeze-dry the mycelia and fermentation liquid, and pass 20 minutes after crushing. Mesh sieve to obtain the fermented product comprising the active substance;

[0055] 3) Add the fermented product from step 2) into distilled water at a solid-to-liquid ratio of 1:8g / mL, extract at 85°C for 5 hours to obtain the extract, concentrate it to extract, freeze-dry, crush and pass through a 20-mesh sieve, that is Cicada flower active substances;

[0056] The above-mentioned liquid fermentation medium is a PDA liquid medium containing 2 ...

Embodiment 2

[0058] The preparation method of cicadae active substance, comprising,

[0059] 1) Inoculate the Cicada japonica strain activated in the PDA slant medium into the seed liquid medium, and culture it on a shaker at 25°C and 120r / min for 3 days to obtain the seed liquid;

[0060] 2) Inoculate the seed solution of step 1) in a liquid fermentation medium with an inoculum amount of 10%, cultivate it on a shaking table at 25°C and 80r / min for 3 days, freeze-dry the mycelia and fermentation broth, crush and pass through 60 Mesh sieve to obtain the fermented product comprising the active substance;

[0061] 3) Add the fermented product from step 2) into distilled water at a solid-to-liquid ratio of 1:12g / mL, extract at 90°C for 6 hours to obtain an extract, concentrate it into an extract, freeze-dry, crush it and pass it through a 60-mesh sieve, that is Cicada flower active substances;

[0062] The above-mentioned liquid fermentation medium is a PDA liquid medium containing 5 g / L of ...

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Abstract

The invention provides an active isaria cicadae miquel substance and an application thereof in prevention, delaying or treatment of cataract, and belongs to the technical field of active substances. A preparation method of the active isaria cicadae miquel substance comprises steps as follows: isaria cicadae miquel strains activated in a PDA (potato dextrose agar) slant medium are inoculated into a seed solution medium for culture, and a seed solution is obtained; the seed solution is inoculated into a PDA liquid medium containing traditional Chinese medicine extract and Cajanonic acid A for culture, then mycelia and fermentation liquor are dried and smashed, and a fermented product containing the active substance is obtained; the fermented product is extracted in distilled water, an extracting solution is obtained and concentrated into extractum, the extractum is dried, and the active isaria cicadae miquel substance is obtained. Active ingredients in the active isaria cicadae miquel substance obtained with the preparation method can play the gain role, and the active isaria cicadae miquel substance has a protecting effect, delays the pathological process of posterior capsule opacification, delays development of cataract and is expected to be applied to prevention and treatment of age-related cataract and after cataract.

Description

technical field [0001] The invention belongs to the technical field of active substances, and in particular relates to the active substance of cicadae flower and its application for preventing, delaying or treating cataract. Background technique [0002] Cataract refers to the disorder of lens metabolism due to various reasons such as aging, local nutritional disorders, immune and metabolic abnormalities, trauma, poisoning, radiation, etc., which cause lens protein denaturation and turbidity, and eventually lead to vision loss or even loss of vision, which seriously affects patients. daily life and quality of life. With the aging population in our country, cataract has become one of the most important blinding eye diseases. At present, the most important way to treat cataract is to perform lens replacement, that is, to remove the cloudy lens and implant an artificial lens. Although cataract surgery technology has gradually matured, surgical treatment not only brings huge e...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P27/12C12N1/14C12R1/645A61K35/64
CPCA61K35/64A61K36/068A61P27/12C12N1/14
Inventor 车有泉郑宝华杨志军
Owner 兰溪市立顺生物有限公司
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