Detection method of isaria cicadae miquel

A detection method, cicada flower technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low extraction rate of adenosine, interference, etc.

Inactive Publication Date: 2017-05-24
ZHEJIANG BIOASIA PHARMA CO LTD
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, "The Pharmacopoeia of the People's Republic of China" 2010 Edition, Part 1 and "Technical Specifications for Health Food Inspection and Evaluation" 2003 Edition of the Ministry of Health of the People's Republic of China are all involved, but the in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of isaria cicadae miquel
  • Detection method of isaria cicadae miquel
  • Detection method of isaria cicadae miquel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] A sample preparation method for cicadae detection, the method comprises the following steps: take cicadae fruiting body powder 0.2g, accurately weighed, put 20mL stopper test tube, add 5mL distilled water accurately, ultrasonic (40KHz) process 30 minutes, take out , Immediately add 5mL of methanol precisely, mix well, pass through a 0.22μm microporous membrane, and take the subsequent filtrate to obtain.

Embodiment 2

[0110] The sample preparation method is basically the same as in Example 1, except that 4.5 mL of methanol is precisely added, and the extraction method is reflux extraction for 30 min.

Embodiment 3

[0112] The sample preparation method is basically the same as in Example 1, except that 4 mL of methanol is precisely added.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a detection method of isaria cicadae miquel. According to the detection method, high performance liquid chromatography is adopted. The detection method comprises following steps: a, determination of chromatographic conditions, wherein an acetonitrile-dihydric phosphate solvent system is taken as a mobile phase, and isocratic elution is adopted; b, a reference solution is prepared, wherein an adenosine reference substance is mixed with a solvent so as to obtain the reference solution; c, a sample solution to be detected is prepared, wherein an isaria cicadae miquel sample to be detected is mixed with a solvent so as to obtain the sample solution to be detected; and d, measuring is carried out, wherein the reference solution and the sample solution are injected into a liquid chromatograph respectively for measuring. The detection method can be used for detecting isaria cicadae miquel sporocarp, spore powder, mycoplasm, or extracts or preparations of isaria cicadae miquel sporocarp, spore powder, and mycoplasm; the detection method is simple; few influences are caused; and stability is high.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of cicadae. Background technique [0002] Cicadae (Isaria cicadae Miquel) belongs to the kingdom Fungi, phylum Ascomycota, subdivision Discomycotina, class Copromycetes, order Hypocreales, family Cordyceps, genus Isaria. [0003] Cicada is a precious Chinese medicinal material in my country, and it is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are: adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. [0004] Cicada has the function of regulating human immunity, improving appetite, promoting sleep, and enhancing the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06
Inventor 张忠亮王玉芹刘艳菊尹彬陈桃宝胡建峰施伟勤顾玲玲徐煜孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap