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Isaria cicadae miquel spore powder artificial culture method

A technology of artificial cultivation and spore powder, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of low yield, difficult cultivation method, long time consumption, etc., achieve good quality, shorten the cultivation period, reduce energy consumption effect

Active Publication Date: 2017-04-05
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation method is difficult and the yield is low, and it takes a long time

Method used

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  • Isaria cicadae miquel spore powder artificial culture method
  • Isaria cicadae miquel spore powder artificial culture method
  • Isaria cicadae miquel spore powder artificial culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Liquid Culture

[0046] Medium formula: 0.5% yeast powder extract powder, 1% compound amino acid, 3.5% white sugar, and make up the rest to 100%;

[0047] Cultivation method: shake flask culture; culture conditions are 72 hours in a shaker at 22°C and 150 rpm; stop the culture when the culture solution turns purple or small mycelium balls in the shape of rice grains appear to obtain seed liquid.

Embodiment 2

[0048] Example 2 liquid culture

[0049] The medium formula and cultivation method are the same as those in Example 1, except that 3% wheat bran boiling juice is added to the cultured seed solution.

Embodiment 3

[0050] Example 3 liquid culture

[0051] The medium formula and cultivation method are the same as those in Example 1, except that 3% soybean powder is added to the seed solution obtained from cultivation.

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Abstract

The invention relates to an isaria cicadae miquel spore powder artificial culture method. The isaria cicadae miquel spore powder artificial culture meth is characterized by comprising following steps of step 1, making a moisture-absorption carrier be fully contacted with strain seed liquid obtained by liquid culture; step 2, carrying out dark culture on the moisture-absorption carrier contacted with the seed liquid first, and carrying out light culture on the moisture-absorption carrier after the moisture-absorption carrier is full of mycelium; and step 3, harvesting spore powder. According to the invention, a moisture-absorption material is taken as the carrier to culture spore powder, the process is simple, the period is short, the sporulation quantity is high, the quality of spore powder is high, and compared with existing solid culture, the isaria cicadae miquel spore powder artificial culture method has obvious advantages.

Description

technical field [0001] The invention relates to a method for artificially cultivating cicadae spore powder, in particular to a method for cultivating cicadae spore powder with a hygroscopic material as a carrier. Background technique [0002] Cicada (Isaria cicadae Miquel) belongs to Ascomycot, Pezizomycotina, Sordariomycetes, Hypocreales, Cordycipitaceae of Fungi (FUNGI). , Isaria (Isaria), is my country's famous and precious Chinese medicinal materials, is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. Cicada has unique and extensive effects, such as improving kidney function, anti-tumor, regulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 陈奇超孙长胜桂海龙龚倩李成褚勤芳
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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