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Preparation method of beautiful wine of Isaria cicadae

A cicada flower and beautiful technology, applied in the field of cicada flower wine preparation, can solve the problems of long cultivation period, poor shape and appearance, uneven growth, etc., and achieve the effect of clear wine color, stable and consistent properties, and high commercial value

Active Publication Date: 2017-03-29
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this brewing method, the cultivation period is very long, and the growth is uneven, the height is different, the density is different, and the shape and appearance are poor.

Method used

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  • Preparation method of beautiful wine of Isaria cicadae
  • Preparation method of beautiful wine of Isaria cicadae
  • Preparation method of beautiful wine of Isaria cicadae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 liquid culture

[0050] Slope culture: Inoculate the isolated and purified cicadae strains into the inclined test tube, then put the inclined test tube inoculated into the 22°C incubator, and the cultivation time is 7 days, until the mycelium is full of the test tube;

[0051] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;

[0052] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, and keep the pressure for 30-45 minutes; after...

Embodiment 2

[0056] Example 2 liquid culture

[0057] Slope culture: Inoculate the isolated and purified cicadae strains into the inclined test tube, then put the inclined test tube inoculated into the 22°C incubator, and the cultivation time is 7 days, until the mycelium is full of the test tube;

[0058] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;

[0059] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, and keep the pressure for 30-45 minutes; after...

Embodiment 3

[0062] Example 3 liquid culture

[0063] Slope culture: Inoculate the isolated and purified cicadae strains into the inclined test tube, then put the inclined test tube inoculated into the 22°C incubator, and the cultivation time is 7 days, until the mycelium is full of the test tube;

[0064] Seed tank culture: put 20L liquid medium in a 50L airlift seed tank, the temperature of the medium is higher than 95°C, add edible defoaming agent, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C and keep the pressure for 30-45 minutes; after sterilization, when it is cooled to 20°C, put 4 bottles of the bacteria in the above-mentioned inclined test tube into the culture medium, the culture temperature is 22±1°C, and the ventilation volume is 1 : 0.5V / V min, holding pressure 4.903-7.0845×10 4 Pa, after 3 days of aerated culture, it can reach the logarithmic growth phase, and the final culture volume is 20L.

[0065]...

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Abstract

The invention relates to a preparation method of beautiful Isaria cicadae wine. The method comprises the followings steps: a step 1: bacterial strains of Isaria cicadae are pressed on the surface of a medium with a porous cover plate in a solid culture process; a step 2: when coremium grows from small pores to a suitable height, coremium at the part where the cover plate and the medium are contacted and the medium are separated; a step 3: the cover plate and coremium which grows in the cover plate are placed in a container together; a step 4: wine is placed in the container; and a step 5: the wine is stored. The preparation method guarantees uniform growth vigor of coremium, eliminates suspension phenomenon of wine due to long time immersion of the medium in the wine, so that quality of the Isaria cicadae wine is guaranteed.

Description

technical field [0001] The invention relates to a method for preparing cicada flower wine, in particular to a method for cultivating cicada flower with a cover plate with holes, and then preparing cicada flower wine. Background technique [0002] Cicadae (Isaria cicadae Miquel) belongs to the kingdom Fungi, phylum Ascomycota, subdivision Discomycotina, class Copromycetes, order Hypocreales, family Cordyceps, genus Isaria. [0003] Cicada is a precious Chinese medicinal material in my country, and it is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are: adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. [0004] Cicada has the function of regulating h...

Claims

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Application Information

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IPC IPC(8): C12G3/04
Inventor 孙长胜陈奇超樊美珍桂海龙龚倩李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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