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Optimized wild Isaria cicadae Miquel strain liquid artificially-culturing method

A technology of artificial culture and liquid strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of bacterial liquid quality and yield impact

Active Publication Date: 2016-05-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

But no matter which cultivation method is adopted, a large amount of high-quality liquid strains need to be obtained. The existing medium formula and cultivation method do not make the wild cicadae mycelia grow optimally in the wild cicadae liquid culture process. The quality and yield of the bacterial solution have had a certain impact
[0005] At present, there is no systematic and effective optimal culture method for screening the optimal carbon source, nitrogen source and micronutrients in the production of wild cicadae liquid strains, so as to provide technical guidance for the large-scale cultivation of wild cicadae

Method used

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Embodiment 1

[0016] A method for optimizing the artificial cultivation of wild cicadae liquid strains, comprising the steps of:

[0017] (1) Optimize the formula of liquid medium: 20g soluble starch, 10g milk powder, 0.5g potassium dihydrogen phosphate, 1g magnesium sulfate, vitamin B1210 tablets, 1000mL water, pH5.0-6.0;

[0018] (2) Optimizing the preparation of liquid medium: Accurately weigh each drug according to the formula in step (1). Take the preparation of 1000mL medium as an example, measure 1000mL of water and boil it with an induction cooker, and then use the formula from easily soluble to insoluble Sequentially add the medicines into the water, stir with a glass rod until dissolved; divide the prepared medium into 500ml Erlenmeyer flasks, each with 150ml, and use a funnel for dispensing when dispensing to avoid the culture medium sticking to the Miscellaneous bacteria are easy to breed on the wall of the mouth of the triangular bottle. If it sticks, wipe it clean with gauze, ...

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Abstract

The invention discloses an optimized wild saria cicadae Miquel strain liquid artificially-culturing method. A liquid culturing medium in an optimized formula is adopted and comprises 20 g of solubility starch, 10 g of milk powder, 0.5 g of monopotassium phosphate, 1 g of magnesium sulfate, 10 pieces of vitamins B12 and 1,000 ml of water, wherein the pH of the liquid culturing medium ranges from 5.0 to 6.0. Wild saria cicadae Miquel strains are artificially cultured; on the basis of a traditional basic culturing medium, a systematic and effective optimized culturing mode for screening optimal carbon sources, optimal nitrogen sources and optimal micronutrients during liquid production of wild saria cicadae Miquel strains is provided, and excellent technical guidance is provided for large-scale cultivation of wild saria cicadae Miquel.

Description

technical field [0001] The invention relates to the field of biological bacteria agents, in particular to an optimization method for artificial cultivation of wild cicadae liquid strains. Background technique [0002] Cicada, also known as cicada, cicada, cicada pupa, chrysalis, etc., is a complex of nymphs and nymphs that parasitize on the nymphs of Cicadae insects. The main components of cicadae are cordycepin, cordycepic acid, cordyceps polysaccharide, amino acid, multivitamins, ergosterol, protein and superoxide dismutase, etc. cicadae and the asexual form of Cordyceps sinensis belong to the same category, and its ingredients and medicinal properties The value is comparable to that of Cordyceps sinensis. It has the effects of analgesia, sedation and hypnosis, antihypertension, two-way immune regulation, anti-tumor generation, anti-inflammation, and improvement of renal function. [0003] The resources of cicadae in nature are very scarce, and because of the severe dama...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 谢春芹曹正高大响许俊齐
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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