Method for culturing Cordyceps militaris by using highland barley as main raw material

A technology of the main raw material, Cordyceps militaris, is applied in the directions of botanical equipment and methods, fertilizer mixtures, horticulture, etc., to achieve the effects of high economic and social benefits, simple formula and simple process

Active Publication Date: 2012-01-04
西藏月王药诊生态藏药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the artificial cultivation of Cordyceps militaris, the main raw materials of the culture substrate used are rice, corn, sorghum, wheat, and there is no report on the cultivation of Cordyceps militaris with highland barley as the main raw material.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The present invention comprises the following steps:

[0024] 1) Select highland barley particles to remove impurities such as sand, stones, metals, etc., wash the highland barley particles with clean water, filter and dry, and dry for later use;

[0025] 2) Prepare nutrient solution:

[0026] Take 50 parts of highland barley granules, 12 parts of glucose, 5 parts of peptone, 1.5 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, 1.5 parts of ammonium citrate, vitamin B 1 0.5 parts, 1000 parts of water to prepare a nutrient solution, adjust the pH value of the nutrient solution to 7-8;

[0027] 3) Preparation of barley growth matrix medium:

[0028] a, adopt the following proportions by weight: 50 parts of highland barley granules, 5 parts of silkworm chrysalis powder, and 30 parts of nutrient solution;

[0029] b. Preparation method: put the above-mentioned raw materials into a culture bottle according to 100ml / bottle, put it into a steam steril...

Embodiment 2

[0038] This embodiment includes the following steps:

[0039] 1) Select highland barley to remove impurities such as sand, stones and metals, wash the highland barley particles with clear water, filter and dry, and dry for later use;

[0040] 2) Prepare nutrient solution:

[0041] Take 60 parts of highland barley granules, 10 parts of glucose, 6 parts of peptone, 1 part of magnesium sulfate, 2.5 parts of potassium dihydrogen phosphate, 1 part of ammonium citrate, vitamin B 1 0.7 parts, 1000 parts of water to prepare a nutrient solution, adjust the PH value of the nutrient solution to 7-8;

[0042] 3) Preparation of barley growth matrix medium:

[0043] a, adopt the following proportions by weight: 80 parts of highland barley granules, 2 parts of silkworm chrysalis powder, and 60 parts of nutrient solution;

[0044] b. Preparation method: put the above-mentioned raw materials into a culture bottle according to 100ml / bottle and put it into a steam sterilizer for sterilizatio...

Embodiment 3

[0053] This embodiment includes the following steps:

[0054] 1) Select highland barley particles to remove impurities such as sand, stones and metals, wash the highland barley particles with clean water, filter and dry, and dry for later use;

[0055]2) Prepare nutrient solution:

[0056] Take 55 parts of highland barley granules, 11 parts of glucose, 5 parts of peptone, 1.2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, 1.2 parts of ammonium citrate, vitamin B 1 0.6 parts, 1000 parts of water to prepare a nutrient solution, adjust the PH value of the nutrient solution to 7-8;

[0057] 3) Preparation of barley growth matrix medium:

[0058] a, adopt the following proportions by weight: 65 parts of highland barley granules, 4 parts of silkworm chrysalis powder, and 50 parts of nutrient solution;

[0059] b. Preparation method: put the above-mentioned raw materials into a culture bottle according to 100ml / bottle and put it into a steam sterilizer for...

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Abstract

The invention discloses a method for culturing Cordyceps militaris by using highland barley as a main raw material, belonging to a fungus culture method in the biotechnology, in particular relates to a method for artificially culturing the fungus, and particularly relates to a method for artificially culturing Cordyceps militaris. The method comprises the following steps of preparing nutrient solution, preparing highland barley growing culture medium, preparing liquid strain, inoculating, culturing, harvesting and the like. The method has the beneficial effects that the highland barley is used as a medium raw material to culture Cordyceps militaris, and the nutrients of the highland barley are absorbed by Cordyceps militaris in the growth phase, so that the content of the effective ingredient cordycepin in the Cordyceps militaris is improved to 1% in the method from the original 0.5%, the content of effective ingredient cordycepic acid is improved to 9% of the product from the existing 6.1%, the content of effective ingredient cordyceps polysaccharide is improved to 10.5% of the product from the existing 7.4%, and the content of the effective ingredients of the Cordyceps militaris is totally improved by 200-250%; and the highland barley is used as the medium raw material to culture the Cordyceps militaris, and thus the obtained Cordyceps militaris has dual effects of the highland barley and the Cordyceps militaris.

Description

Technical field: [0001] The invention discloses a method for cultivating Cordyceps militaris with highland barley as the main raw material, which belongs to the method for cultivating fungi in biotechnology, in particular the method for artificially cultivating fungi, especially the method for artificially cultivating Cordyceps militaris. Background technique: [0002] Cordyceps militaris is a precious Chinese herbal medicine. The wild resources of Cordyceps militaris are very limited, and over-harvesting of wild Cordyceps will cause environmental damage. Therefore, the Cordyceps of wild source can not satisfy the demand of market far away, and artificial cultivation of Cordyceps is in urgent need. People have long begun to study the method of artificially cultivating Cordyceps militaris and put it into practice, and some progress has been made. At present, in the artificial cultivation of Cordyceps militaris, the main raw materials of the culture substrate used are rice, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 赵辉
Owner 西藏月王药诊生态藏药科技有限公司
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