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Universal edible fungus liquid culture growth medium and its manufacturing technology

A technology of liquid bacteria and culture medium, applied in fungi and other directions, can solve the problems of large changes in nutrients, production uncertainty, long bacteria growth cycle, etc., and achieve the effects of strong vitality, increased yield and strong versatility

Inactive Publication Date: 2006-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current edible fungus liquid culture medium generally needs boiled potato juice or bran juice as raw materials, resulting in cumbersome procedures and complicated processes
At the same time, this kind of medium needs to be composed of different formulas for different fermentation objects; not only is it not universal, but also because of the large changes in nutrient components, it is difficult to meet the requirements of stable fermentation, which brings many uncertainties to production. The germination rate of this medium after inoculation is between 50% and 75%, the stagnation period is as long as 48 to 72 hours, and the entire fermentation cycle is 120 to 168 hours
Due to the long germination cycle, the usual yield is about 50%, and the output is unstable and the contamination rate is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, a kind of general-purpose edible mushroom liquid culture medium, the formula component of this medium is: maltodextrin 55Kg, glucose 10Kg, sucrose 10Kg, yeast extract 1 part, peptone 1 part, potassium dihydrogen phosphate 3 1 part, 1 part of magnesium sulfate and 1000 parts of water.

[0013] The manufacturing process of this universal edible fungus liquid strain culture medium is to carry out the above-mentioned formula components according to the following steps:

[0014] 1) Warm water to 90°C, add maltodextrin and stir until dissolved;

[0015] 2), then add glucose, sucrose, yeast extract and peptone in turn, and stir until dissolved;

[0016] 3), then add potassium dihydrogen phosphate and magnesium sulfate in turn, and mix thoroughly to make a mixed solution;

[0017] 4) Sterilize the above mixed solution at 121-126° C. and 0.11-0.14 Mpa for 50-80 minutes.

[0018] Aseptically insert Pleurotus eryngii bacterial classification with substratum of the...

Embodiment 2

[0019] Embodiment 2, a kind of general-purpose edible mushroom liquid culture medium, the formula component of this medium is: maltodextrin 50Kg, glucose 10Kg, sucrose 10Kg, yeast extract 1Kg, peptone 1Kg, potassium dihydrogen phosphate 3Kg, sulfuric acid Magnesium 1.5Kg and water 1000Kg.

[0020] The manufacturing process of this universal edible fungus liquid strain culture medium is to carry out the above-mentioned formula components according to the following steps:

[0021] 1) Warm water to 92°C, add maltodextrin and stir until dissolved;

[0022] 2), then add glucose, sucrose, yeast extract and peptone in turn, and stir until dissolved;

[0023] 3), then add potassium dihydrogen phosphate and magnesium sulfate in turn, and mix thoroughly to make a mixed solution;

[0024] 4) Sterilize the above mixed solution at 121-126° C. and 0.11-0.14 Mpa for 50-80 minutes.

[0025] Use culture medium of the present invention to aseptically insert Flammulina velutipes bacterial cla...

Embodiment 3

[0026] Embodiment 3, a kind of general-purpose edible mushroom liquid culture medium, the formula component of this medium is: maltodextrin 45Kg, glucose 15Kg, sucrose 10Kg, yeast extract 1.5Kg, peptone 1Kg, potassium dihydrogen phosphate 2.5Kg , magnesium sulfate 1.5Kg and water 1000Kg.

[0027] The manufacturing process of this universal edible fungus liquid strain culture medium is to carry out the above-mentioned formula components according to the following steps:

[0028] 1) Warm water to 95°C, add maltodextrin and stir until dissolved;

[0029] 2), then add glucose, sucrose, yeast extract and peptone in turn, and stir until dissolved;

[0030] 3), then add potassium dihydrogen phosphate and magnesium sulfate in turn, and mix thoroughly to make a mixed solution;

[0031] 4) Sterilize the above mixed solution at 121-126° C. and 0.11-0.14 Mpa for 50-80 minutes.

[0032] Insert Coprinus comatus bacterial classification aseptically with substratum of the present invention...

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PUM

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Abstract

The invention discloses a universal edible fungus liquid fungus culture medium.Its weight ratio of the formula is: malt dextrin 45-55 portions, dextrose 10-15 portions, cane sugar 10-15 portions, yeast extract paste 1-1.5 portions, peptone 1-1.5 portions, potassium di-hydrogen phosphate 2-3 portions, bitter salt 1-1.5 portions, and water 1000 portions. The invention also discloses the technology that includes following steps: heating water to 90-95 degree centigrade, adding malt dextrin and whisking until fully solving, adding dextrose, cane sugar, yeast extract paste, and peptone and whisking until fully solving, adding potassium di-hydrogen phosphate and bitter salt and fully mixing to make mixture liquid, which would take high temperature and high pressure sterilization. The invention has strong versatility and short fermentation period.

Description

technical field [0001] The invention relates to a culture medium and a manufacturing process thereof, in particular to a general-purpose edible mushroom liquid strain culture medium and a manufacturing process thereof. Background technique [0002] Culture medium is the basis for the quality of liquid strains. Whether the composition and ratio of the culture medium is appropriate or not has a great influence on the fermentation process of the liquid strain. Only by choosing reasonable culture ingredients and proportions can we quickly cultivate robust mycelium. Stable fermentation of liquid strains is necessary for intensive production, and its premise is a stable medium. The stability of the medium must have standard raw material quality components and standardized formulas, as well as standardized proportioning procedures. The current edible fungus liquid culture medium generally needs boiled potato juice or bran juice as raw materials, resulting in cumbersome procedure...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 陈再鸣
Owner ZHEJIANG UNIV
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