Low-carbon and high-yield poria culture method

A Poria cocos, high-yield technology, applied in the field of low-carbon high-yield Poria cocos cultivation, can solve the problems of insignificant strain yield and low genetic stability of the strain, and achieve the effects of solving the contradiction between strains, reducing strength and improving work efficiency

Active Publication Date: 2013-02-20
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The method for cultivating Poria cocos in the existing literature has been reported, adopting the method of inoculating Poria cocos on the top of the cocos, the side of the cocos and the lateral roots, the yield of the strain is not significant, and the genetic stability of the strain is not high

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1 strain selection

[0020] Poria cocos breeding research using protoplast ultraviolet mutagenesis technology with "Minling" as parent: preparation of "Minling" mycelial protoplasts, and diluting the protoplasts to 1.25×10 6 1 / ml, 0.1 ml each was spread on the CYM regeneration medium, and irradiated with a 15w ultraviolet lamp for mutagenesis. The petri dish was 30 cm away from the lamp tube, and after turning on the lamp to preheat for 20 min, open the dish cover to irradiate for 150 s. After the ultraviolet mutagenesis was completed, the plates were tightly wrapped with black paper, and cultured at 28°C for 5-7 days. After the protoplasts were regenerated, 120 robust and pure colonies (numbered W1-W120) that grew out first were selected, and transferred into PDA medium for culture. Taking the growth rate of mycelium on the PDA plate as the primary screening standard, 30 regenerated strains (numbered P1-P30) with the highest growth rate were screened out. Taking the...

Embodiment 2

[0058] A method for cultivating Poria cocos with low carbon and high yield, the specific operations are as follows:

[0059] 1. Selecting the venue

[0060] Lingchang should be far away from pollution sources. The air quality should meet the GB3095-96 air environment quality level two, the soil quality should reach the GB15618-95 soil environment quality standard level two, and the water source should meet the GB5084-92 farmland irrigation water quality standard. Lingchang should choose to sit north or south. Sitting west and facing east, the altitude is 300-1500M, the slope is 10-50 degrees, full sun, ventilation and no water accumulation, the soil is loose sandbag soil, which is slightly acidic (pH5.5-6).

[0061] Selection: Choose healthy pine bushes with a tree diameter of more than 20 cm that have been felled for 1-2 years.

[0062] 2. Strain production

[0063] 2.1 Strain requirements

[0064] Select the high-quality, high-yielding, strong stress-resistant Poria varie...

Embodiment 3

[0097] Example 3 Comparative test of "Chuanjie No. 1" and "Chuanjie No. 1-A5"

[0098] In the early 1990s, our institute bred the "Chuanjie No. 1" variety through the induction and domestication of wild Poria cocos strains. The early test results of this variety showed that it was also a more suitable variety for the cultivation of Poria cocos pine. However, after several years of cultivation and application It shows that its genetic stability is slightly poor, and the strain has degenerated phenomenon. So in 2000, we used the wild Poria cocos strain "Minling" as the parent, and applied protoplast ultraviolet mutagenesis technology to breed a new Poria cocos variety "Chuanjie No. 1-A5". The yield of Jieling is particularly outstanding, and the average yield per pine can reach 15kg / diameter of more than 25±5cm. In 2010, a comparison test of two varieties of Lingling was carried out in the forest farm of Shuibei Town, Shaowu County. The statistical analysis of the test results ...

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Abstract

The invention relates to a low-carbon and high-yield poria culture method and belongs to the technical field of edible (medicinal) fungus culture. According to the method, poria is cultured naturally by utilizing underwood waste pine root and stem, and a specific poria strain, which is Wolfiporia extents Chuanjie-1-A5 with preservation number of CGMCC No.6660, suitable to be cultured by using pine root and stem is selected and is cultured in combination with a taproot inoculation method. The culture method comprises the following steps of: producing a strain; pre-treating the pine root and stem; inoculating; and conventionally managing and harvesting. According to the method, waste pine root and stem of forestry is effectively utilized to naturally culture poria in a low-carbon manner, 'conflict between fungus and forest' in poria production can be effectively solved, and harm of termite and other insect pests caused by forest waste discharge can be reduced, so that the production cost can be reduced, labor intensity of poria planting can be greatly reduced, time and labor can be saved, and management is facilitated. The culture method is beneficial to sustainable development of the poria industry and has remarkable economical benefit.

Description

[0001] technical field [0002] The invention belongs to the technical field of cultivation of edible (medicine) fungi, and more specifically relates to a method for cultivating poria cocos with low carbon and high yield Background technique [0003] Poria [ Wolfiporia extensa (Peck) Ginns] also known as Poria, Yunling, Fuling, Songshu, Songling and so on. In taxonomy, it belongs to Basidiomycota, Agaricaceae, Polyporacea, Polyporaceae, and Poria. Poria cocos usually refers to the sclerotium that parasitizes on the roots of Pinaceae plants. Poria cocos has a medicinal history of more than 2,000 years in my country, and is currently one of the varieties with the largest production of medicinal fungi in China. Although Poria cocos has been cultivated artificially for more than 1,000 years, the traditional mode of planting Poria cocos is still followed in cultivation. According to incomplete statistics from the pharmaceutical industry, more than 20,000 tons of dried Poria c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 蔡丹凤陈丹红郑朋武林佩瑛蔡志欣陈美元王泽生
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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