Method for isolating strains of Cordyceps sinensis in asexual stage

A technology of Cordyceps sinensis fungus and asexual stage, which is applied in the field of bacterial strain separation, can solve the problems of easy pollution, high pollution rate, complicated collection procedures, etc., and achieves the effect of realizing recycling, low bacterial pollution rate and saving biological materials.

Inactive Publication Date: 2011-12-21
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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Problems solved by technology

The spore separation method is not easy to obtain the newly mature fruiting bodies of ascospores in the wild, and the collection procedures are complicated and easily polluted during the process, and it is even more difficult to obtain a single spore
Therefore it is difficult to obtain the pure strain of Cordyceps sinensis with this method of isolation and cultivation
The tissue separation method not only has a slow growth rate, but also has many types of bacteria and a high contamination rate during the separation process, making it difficult to obtain pure strains of Cordyceps sinensis. Because of this, the names of the strains isolated by many domestic units since the 1980s are different. , different forms, but they all think that what they have isolated is the real strain of Cordyceps sinensis, but the real situation is that most of the reports are not real Cordyceps sinensis.

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  • Method for isolating strains of Cordyceps sinensis in asexual stage
  • Method for isolating strains of Cordyceps sinensis in asexual stage
  • Method for isolating strains of Cordyceps sinensis in asexual stage

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[0015] Weigh 200g of fresh potato pieces, 200g of glucose, 20g of agar, 15g of peptone, 0.5g of magnesium sulfate, 0.5g of potassium dihydrogen phosphate, and 5g of yeast powder; Put it in a Erlenmeyer flask, add water to 1000ml, then add other ingredients, shake well, heat and boil until the agar powder is fully dissolved, then pack it into a triangular flask, seal it with a cotton plug and kraft paper, and put it into a steam sterilizer sterilize at 115°C for 30 min under a pressure of 100 kPa, take it out after cooling down to room temperature, and put it on an ultra-quiet workbench under aseptic conditions into sterile glass empty plates with a diameter of 90 mm, cover Put on the cover, cool naturally to make it solidify, and obtain plate culture medium, and store it in a refrigerator at 4°C until use.

[0016] Live larvae of bat moths infected with Trichospora sinensis were collected from the artificial cultivation bases of Cordyceps sinensis in Kangding and Shiqu, Sichua...

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Abstract

The invention provides a method for isolating the asexual stage bacterial species of Mortierella sinensis from the body fluid of the living bat moth larvae infected with Mortierella sinensis; Mortierella sinensis, a live bat moth larva infected with Mortierella sinensis, can be used to isolate and cultivate the asexual stage strains of Cordyceps sinensis many times. Compared with the spore separation method and tissue separation method, the operation is simpler, the contamination rate of miscellaneous bacteria is low, and biological materials are saved. The bat moth larvae that have been extracted from the body fluid can also grow Cordyceps sinensis through continuous feeding. The recycling of biological propagation materials in the cultivation process of Cordyceps sinensis is realized.

Description

technical field [0001] The invention relates to a method for isolating strains, in particular to a method for isolating Cordyceps fungus from bat moth larvae. technical background [0002] Cordyceps sinensis is a traditional precious nourishing Chinese medicinal material, which is listed as the three major nourishing products together with natural ginseng and deer antler. It has mild medicinal properties and can be eaten all year round. It is suitable for the old, young, sick, weak, and weak. It has wider medicinal value than other types of tonics. Demand at home and abroad is increasing day by day. Cordyceps sinensis is an ascomycete that has a conidium stage (anamorph) and an ascospore stage (sexual type) in its life cycle. The strains of Cordyceps sinensis used in actual production such as artificial cultivation and liquid fermentation are all in the asexual stage, and the correct identification of the strains is very important, so the problem of its asexual type is par...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 尹定华曾纬陈仕江刘飞涂永勤张德利李黎马开森罗庆明钱敏
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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