30 results about "Spore coat" patented technology

Germicidal compositions with enhanced activity towards killing microbiological spores and vetgetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.

The present invention relates to a method for display of proteinson spore surface and a method for improving protein with rapidity using the same, which comprises the steps of (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface the protein of interest.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Germicidal compositions with enhanced activity towards killing microbiological spores and vetgetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.

The present invention concerns spore display methods. More specifically, the invention concerns display of heterologous molecules, such as peptides and polypeptides, on spores of bacilli, such as, for example, Bacillus thuringiensis (Bt) or Bacillus cereus (BC), using externally exposed spore coat proteins or fragments or variants thereof.

The invention provides a method for isolating the asexual stage bacterial species of Mortierella sinensis from the body fluid of the living bat moth larvae infected with Mortierella sinensis; Mortierella sinensis, a live bat moth larva infected with Mortierella sinensis, can be used to isolate and cultivate the asexual stage strains of Cordyceps sinensis many times. Compared with the spore separation method and tissue separation method, the operation is simpler, the contamination rate of miscellaneous bacteria is low, and biological materials are saved. The bat moth larvae that have been extracted from the body fluid can also grow Cordyceps sinensis through continuous feeding. The recycling of biological propagation materials in the cultivation process of Cordyceps sinensis is realized.

The invention provides a spore genetically modified with genetic code comprising at least one genetic construct encoding an antigen and a spore coat protein as a chimeric gene, said genetically modified spore having said antigen expressed as a fusion protein with said spore coat protein.

The invention discloses a method for producing N-acetylneuraminic acid by a spore surface display system. The method comprises the following step of: after recombining with a spore coating protein gene and an N-acetylneuraminic acid aldolase gene by utilizing a high-copy shuttle vector, constructing a surface display expression carrier; and converting the surface display expression carrier into bacillus subtilis to obtain a recombined strain, wherein a spore of the recombined strain can catalyze the synthesis of the N-acetylneuraminic acid. Compared with other methods in the field, the invention can finish the expression, purification and immobilization of enzyme at one step and has concise and efficient operating process. In addition, by utilizing the characteristics of stable spore, easy separation and stress resistance of the bacillus subtilis, the following separating process is simplified, the stability of N-acetylneuraminic acid aldolase is improved, and the safety of the whole catalyzing process is improved.

The invention relates to an application of bacillus subtilis spore coat proteins CotX, in particular to the application of utilizing bacillus subtilis spore coat proteins CotX as a protein carrier adopting a spore surface to display exogenous proteins, which builds recombinant spores with surfaces displaying exogenous proteins through the spore surface displaying technique. The application is characterized by utilizing coding gene cotX of proteins of the carrier to perform gene recombination with coding sequences of exogenous proteins genes, building integrity recombinant plasmid fusing and expressing recombinant proteins, screening recombination strain after transforming bacillus subtilis, inducing the recombination strain to generate spores, and then obtaining the recombinant spores with surfaces displaying exogenous recombinant proteins.

The invention belongs to the field of the preparation of a biological material, and relates to a preparation method and an application of a bacterial spore functional microsphere. By virtue of a novel biochemical method, bacterial spores are processed so as to prepare a functional microsphere which is high in dispersity, and a nano material is immobilized on the surfaces of the processed pores; the functional microsphere can be applied to various fields. The preparation method is characterized by comprising the following steps: collecting bacterial spores which are cultivated for 3-10 days and are at 108-1011cfu in concentration; carrying out centrifugal washing and ultrasonic treatment; processing the bacterial pores with a trypsin liquid and a spore coating removal processing fluid, so as to finally obtain a bacterial spore microsphere that spore exosporium and spore coating are removed and surface is smooth; the microsphere is used for loading metal nanoparticles. The functional microsphere prepared by the invention is more stable in mechanical rigid structure, the loaded nano material is not easy to peel off, and the functional microsphere, as a carrier, is more stable and reliable in performance. The invention also discloses an application of the prepared spore microsphere in an animal immunization detection material.

The invention discloses a method for producing N-acetylneuraminic acid by a spore surface display system. The method comprises the following step of: after recombining with a spore coating protein gene and an N-acetylneuraminic acid aldolase gene by utilizing a high-copy shuttle vector, constructing a surface display expression carrier; and converting the surface display expression carrier into bacillus subtilis to obtain a recombined strain, wherein a spore of the recombined strain can catalyze the synthesis of the N-acetylneuraminic acid. Compared with other methods in the field, the invention can finish the expression, purification and immobilization of enzyme at one step and has concise and efficient operating process. In addition, by utilizing the characteristics of stable spore, easy separation and stress resistance of the bacillus subtilis, the following separating process is simplified, the stability of N-acetylneuraminic acid aldolase is improved, and the safety of the whole catalyzing process is improved.

Germicidal compositions with enhanced activity towards killing microbiological spores and vetgetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.

The invention discloses an application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as a main pathogen of bombyx mori. The Ag nanoparticles are spherical, are 3-24nmin dimension and are 0.23nm in crystal lattice distance. Through Ag nanoparticle solution of different concentrations, the toxicity of bombyx mori larvae is tested, and the result shows that the weight, the cocoon shell rate and the oviposition number of the bombyx mori larvae between groups do not have significant differences, which indicates that the Ag nanoparticle solution having the concentration being 100 [mu]g / mL or below is safe to the bombyx mori larvae; a mechanism of Ag nanoparticles for resisting nosema bombycis is analyzed, then the inventor finds that the Ag nanoparticles can seriously destroy the structure of the nosema bombycis, when the Ag nanoparticles act with spore coats, the permeability is increased, so that some substances of DNA, proteins and the like are destroyed, the infestation capacity is greatly reduced, the pathogenic capacity of the nosema bombycis is reduced, and the disinfection effects of the nosema bombycis can be achieved; and the Ag nanoparticleshave important significance in controlling the nosema bombycis of the bombyx mori.

The invention belongs to the field of the preparation of a biological material, and relates to a preparation method and an application of a bacterial spore functional microsphere. By virtue of a novel biochemical method, bacterial spores are processed so as to prepare a functional microsphere which is high in dispersity, and a nano material is immobilized on the surfaces of the processed pores; the functional microsphere can be applied to various fields. The preparation method is characterized by comprising the following steps: collecting bacterial spores which are cultivated for 3-10 days and are at 108-1011cfu in concentration; carrying out centrifugal washing and ultrasonic treatment; processing the bacterial pores with a trypsin liquid and a spore coating removal processing fluid, so as to finally obtain a bacterial spore microsphere that spore exosporium and spore coating are removed and surface is smooth; the microsphere is used for loading metal nanoparticles. The functional microsphere prepared by the invention is more stable in mechanical rigid structure, the loaded nano material is not easy to peel off, and the functional microsphere, as a carrier, is more stable and reliable in performance. The invention also discloses an application of the prepared spore microsphere in an animal immunization detection material.

The invention provides a production method of hirsutella sinensis fermented lycium barbarum. The invention relates to a production method of fermented lycium barbarum, which is mainly applied to hirsutella sinensis fungus fermented lycium barbarum. The production method is characterized by comprising the following steps: Step 1, preparing a culture medium from 30 percent to 60 percent of lycium barbarum and 40 percent to 70 percent of milk in percentage by weight, placing the culture medium into a culture container, enabling the culture medium to account for one tenth to seven tenths of the volume of the culture container, carrying out moist heat sterilization for 30 minutes at a temperature of 115 to 140 DEG C, adding a hirsutella sinensis strain which accounts for 0.5 percent to 1 percent of the weight of the culture medium, culturing for 3 to 15 days at a temperature of 15 to 22 DEG C and after hyphae full grow on the surface of the culture medium, completing the fermenting process; and Step 2, drying, grinding and sieving substances in the container to obtain the hirsutella sinensis fermented lycium barbarum product. The invention implements the production method of the hirsutella sinensis fermented lycium barbarum for the first time; the hirsutella sinensis fermented lycium barbarum has an obvious kidney invigorating effect; and the production method is easy for large-scale industrial production and is a green and safe production method.

The invention relates to a processing technology of licuid ganoderma spore powder, in particular to a method for sufficient releasing licuid ganoderma spore powder. It adopts microwave softening treatment method. The concrete process is as follows: 1.placing raw materials licuid ganoderma spore powder into the microwave reaction chamber, adding organic acid solution and stirring sufficiently to make them wet homogenously 2.microwave treatment, applying microwave power to strengthen the reaction between the organic acid solution with chitin of the spore coat structure and colloid, enabling them partial solved and producing dense distributed nanometer holes or cracks on the spore coat. 3. vacuum distilling and removing organic acid solution. Said microwave power applying method combines he continuous microwave and impulse microwave, at first continuous microwave is applied to radiate, then the impulse microwave is applied when the organic acid solution refluxes, the irradiation time is 5min to 100min. The invention not only promotes the release capability of the effective constituent(in particular for polysaccharide) in the spore powder, but also ensures the integrity of the integral appearance of the spore powder. The loss of the easy oxidation effective constituent is also avoided.

The invention belongs to the field of biological material preparation, and particularly relates to preparation of spore@Zr<4+> as functional microspheres and applications of spore@Zr<4+> in immunoassay. According to the present invention, spores are treated by using a biochemical method to prepare suspension microspheres with high stability and good dispersion, Zr<4+> ions are adsorbed onto the surfaces of the spores, and the obtained spore@Zr<4+> is used in the field of immunoassay; bacterial spores cultured for 7 days are collected, centrifugation washing and ultrasonic treatment are performed, treatment is performed with a trypsin solution and a spore coat removing treatment solution to obtain bacterial spore microspheres with characteristics of smooth surface, spore wall removing and spore coat removing, and the bacterial spore microspheres adsorb Zr<4+> ions to prepare the spore@Zr<4+> functional microspheres; the prepared spore@Zr<4+> functional microspheres have characteristicsof good mechanical strength, uniform particle size, proper specific gravity, simple preparation process, low cost, high-throughput detection and environmental protection; and the invention further discloses the applications of the spore@Zr<4+> microspheres in the immunoassay of Mycobacterium bovis infection.

The invention discloses a method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture. The method comprises the four steps of strain selection, mushroom dreg acquisition, mushroom dreg growth and conidiophore induction. According to the method, high-stability high-activity aweto single ascospores capable of fermenting a large amount of mycelia are used as a strain; and the hyphae are subjected to multistage propagation and subjected to photoperiod stimulated culture by using different light sources in the conidiophore induction stage, thereby obtaining the high-yield Hirsutella sinensis conidiophores (1011-1012) within a short time. Thus, the method has important meanings for industrialized development of aweto artificial culture.

The present invention relates to a composition of granulated or pelletized endomycorrhizal fungi spores coated with a covering of mineral clays and binders with calcium sulphate monohydrate and the production and use thereof in agriculture.

The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the Bacillus spore coat protein CotA.

The invention discloses a method for the long-term preservation of Hirsutella sinensis. The method comprises the steps of: separating a mycelia from Cordyceps sinensis, and then culturing the mycelia on a PDA slant, then inoculating the mycelia cultured on slant onto PDA plates, culturing at 18 DEG C for 30 days. Cutting into agar blocks with mycelia by using a scalpel, transferring the agar blocks to sterile 10-20% glycerol cryovials, sealing the cryovials with a sealing film, and storing in a refrigerator with temperature of -70 DEG C. The problems of the variation of the strain and the contamination rate due to the multiple times of inoculation are reduced by the method disclosed by the invention.

The invention relates to a laccase which comes from corbrin-capsule-producing strain cordyceps sinensis hirsutella sinensis and participates in degradation of lignin and phenols, a gene encoding the laccase, and application of the above two. The amino acid sequence of the laccase and protein shown as SEQ ID No. 1 have 90% or more of homology, and the nucleotide sequence of the encoding gene and a sequence shown as SEQ ID No. 2 have 90% or more of homology. According to the technical scheme, detailed research is performed on synthesis of benzoquinones form phenols in a principle level, the laccase which comes from corbrin-capsule-producing strain cordyceps sinensis hirsutella sinensis and participates in degradation of phenols, and the encoding gene are provided. A cloned DNA of the provided nucleotide sequence can be transferred into an engineering bacterium through a transduction, transformation or conjugal transferring method. By adjusting expression of the encoding gene corresponding to the enzyme catalyzing phenols for preparing corresponding benzoquinones, a host is endowed with laccase high-expression property, an effective approach is provided for expanding biological application of laccase, and important application prospect is provided.

The invention relates to a phospholipase C in a corresponding free fatty acid, which is prepared by hydrolyzing phospholipids via hirsutella sinensis of Cordyceps sinensis (Berk.) Sacc. from 'Corbrin' producing strains, and a gene for coding the phospholipase C as well as an application of the phospholipase C. The homology between an amino acid sequence of the phospholipase C and a protein shown as SEQ ID No.1 reaches above 90%, and the homology between a nucleotide sequence of the coded gene of the phospholipase C and a sequence shown as SEQ ID No.2 reaches above 90%. The cloned DNA with the nucleotide sequence, which is provided by the invention, can be transformed into engineering bacteria via methods of transduction, transformation and combined transferring, so that high expressivity of a host laccase is realized by regulating the expression of the corresponding coded gene of the enzyme which is contained in the corresponding free fatty acid and prepared by the catalytic hydrolysis of the phospholipids, thereby providing an effective way for enlarging biological applications of the phospholipase C. Thus, the phospholipase C has a great application prospect.

The invention belongs to the technical field of genetic engineering, and particularly relates to a gold ion detection and adsorption system, host bacteria thereof and a gold ion recycling method. The gold ion detection and adsorption system comprises a detection unit and an adsorption unit connected with the detection unit, the detection unit comprises a fluorescent protein DNA sequence, and the fluorescent protein DNA sequence is connected with a first promoter; the adsorption unit comprises a bacillus subtilis spore coat protein CotC DNA sequence and a gold ion binding protein GolB DNA sequence which are mutually connected, and the bacillus subtilis spore coat protein CotC DNA sequence is connected with a second promoter; the detection unit is located at the upstream of the adsorption unit. According to the gold ion detection and adsorption system, host bacteria thereof and the gold ion recycling method, detection and recyclable enrichment and recycling of heavy metal gold in industrial wastewater can be achieved through a biological means, the disadvantages of the prior art can be overcome, secondary contamination cannot be caused, and the gold ion detection and adsorption system is environmentally friendly.

The present invention relates to a preparation method of Mortierella sinensis mycelium polysaccharide, comprising: S1: Extraction of Mortierella sinensis mycelium polysaccharide: using Mortierella sinensis mycelium as raw material, ultrafine pulverization until the particle size is 7-11μm ultra-fine powder, added to water, soaked, then heated to boiling, kept slightly boiled, centrifuged, and the supernatant was obtained as the crude extract of Mortierella sinensis mycelia polysaccharide; S2: Concentrated to obtain a concentrated solution ; S3: precipitation: adding ethanol, stirring while adding, standing still, centrifuging, and collecting the precipitate; S4: drying: freeze-drying to obtain the polysaccharide of Mortierella sinensis mycelia. The method is simple, efficient, low in cost, high in polysaccharide yield and content, and suitable for large-scale production.

The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the Bacillus spore coat protein CotA.

The invention relates to a method for acquiring a high quality Hirsutella sinensis strain. According to the method, a Hirsutella sinensis strain is acquired from a ghostmoth larva digestive tract (mainly intestinal juice) infected with Hirsutella sinensis bacterium. The method is simple to operate and has no damage to ghostmoth larvae, digestive juice (mainly intestinal juice) collected larvae can further grow normally, and can grow into Ophiocordyceps sinensis. Also, the obtained strain can directly serve as an initial strain of industrial fermentation without screening and induction, and the mycelium yield is high.

The invention discloses an oral powder, which is composed of spores, cocci, aspergillus, glucose, carrot juice and fruit juice. The invention is suitable for drinking by ordinary people. Compared with other oral powders, it can protect the gastrointestinal tract in the human body, inhibit the activity of Escherichia coli and indolebutyric acid bacteria in the gastrointestinal tract, and make the food entering the gastrointestinal tract decompose in a normal state. . Thereby enhancing the immunity of the human body, improving sleep status, and having a certain auxiliary effect on reducing hyperglycemia, hyperlipidemia, and renal failure, and promoting the recovery of health for postoperative patients. Pre-cancer has better treatment and adjuvant therapy effect.

The invention discloses a surface active complex enzyme, bionic peat humic acid as well as a preparation method and application of the surface active complex enzyme. The preparation method of the surface active complex enzyme comprises the following steps: inoculating a mixed culture medium with a mixed strain for fermentation culture, wherein the mixed strain comprises yeast, rhizobium, penicillium, lactobacillus plantarum and bacillus subtilis, and the mixed culture medium is a mixture of PDA culture medium and an MRS culture medium. The surface active complex enzyme can line a reaction interface under a micro water or hydrophilic condition, remove harmful bacteria, reduce external energy supply and rapidly oxidize or ectopically condense straw lignin into humic acid. The bionic peat humic acid can be applied to rice, vegetables, flower seedling culture and cultivation media, and can also be applied to removal of rice field moss spore coats, humic acid bio-organic fertilizer, soil improvement remediation agents, biological stimulin, Chinese herbal medicines in soiless culture planting as well as rapid fermentation for returning to the field.