9 results about "Spore coat" patented technology

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

A modified sporulating bacteria may comprise a nucleic acid cassette having a silent promotor that inhibits transcription by the modified sporulating bacteria. The nucleic acid cassette may include a nucleic acid sequence including a gene of interest that encodes a protein of interest. The nucleic acid cassette may include a nucleic acid sequence encoding a loading peptide that incorporates into a coat of a spore. The modified sporulating bacteria may comprise a pre-spore having the nucleic acid sequence having the gene of interest that encodes the protein of interest. A modified spore may be formed comprising a spore body containing the nucleic acid cassette, a spore coat, and a protein of interest derived from the gene of interest inserted into the spore coat made by exogenous cell-free biosynthesis components.

The application discloses a recombinant bacillus subtilis of surface display of a fusion protein of Edwardsiella tarda outer membrane protein OmpA and Vibrio cholerae enterotoxin CTB, a construction method and application. A homologous double cross recombinant of coding genes of OmpA and CTB is carried out into a genome of the bacillus subtilis 168 by taking the spore coat protein CotY as an anchor point, so that the recombinant bacillus subtilis capable of stably inheriting in the bacillus subtilis and displaying the Edwardsiella tarda outer membrane protein OmpA and the Vibrio cholerae enterotoxin CTB on a spore surface is obtained. After fish and mammals orally immunize the bacillus subtilis, the survival time and survival rate of the fish and mammals after infection of the Edwardsiella tarda are obviously improved. The recombinant bacillus subtilis is used as an oral vaccine type micro-ecological immunization preparation for preventing and treating the Edwardsiella tarda infection of the fish and mammals, so that the immunization procedure is obviously simplified, the immunization stress is reduced, the labor cost is saved, the effective concentration in the intestinal tract is ensured, and the specific immune response of the body is induced.

The present application relates to a kind of recombinant bacillus subtilis of PRRSV ER protein on spore surface display, construction method and application.The recombinant bacillus subtilis is by genetic engineering method, with integrated plasmid pDG364 as foundation, constructs a recombinant vector with spore coat protein CotB as anchor protein, surface display PRRSV ER protein, is obtained by chemical transformation method with the recombinant vector is transformed into wild type bacillus subtilis 168, it has genetic stability, and will not be lost in the process of passage.The antigen protein is directly displayed on the spore surface of the bacterium, without crushing, can be directly mixed with material or through drinking water immunization animal, avoid the cumbersome process of large-scale injection.Benefiting from the good stress resistance of spore, the bacterium is easy to preserve and transport, significantly reduce cost.The bacterium can induce specific immune response, which provides a new way for the prevention and treatment of PRRSV, suitable for the development of commercial vaccine.

The application discloses a spore killing method and application thereof, and the method is to add glucosamine to mix with spores and then to perform heat treatment to efficiently inactivate the spores. According to the technical scheme of the application, the spore cortex is first damaged by glucosamine to release DPA in the nucleus, the DPA destroys the disulfide bond on the surface of the spores, and then the integrity of the spore coat is destroyed to reduce the resistance of the spores, and the killing of the spores is realized by cooperating with a mild heat treatment step; compared with the prior art, the spores can be inactivated by 100% by using a lower heat treatment temperature, and better sterilization effect is achieved by lower energy consumption and cost. The method is applied to the field of food processing, and can provide a novel, effective, highly applicable and more practically applicable spore killing method for the storage of food and the keeping of quality, flavor and nutrient components of the food.

The present application belongs to the field of fish molecular immunology, and particularly relates to an oral vaccine containing a largemouth bass iridovirus ATPase and a preparation method thereof. B. subtilis The spores of the WB600 strain are antigen delivery carriers, the spore coat protein C (CotC) is used as an anchor protein, and the key protein of the largemouth bass iridovirus is fused with the ATPase gene and CotC the gene to construct a recombinant spore surface display system, so that the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. The vaccine can tolerate the gastrointestinal environment after being orally administered to fish, and is accurately delivered to the gut-associated lymphoid tissue to stimulate the immunity of the fish, so that the prevention and control of the largemouth bass iridovirus is finally achieved.

The invention discloses an inflammation-targeted drug-loaded microgel precursor based on probiotic spores and a preparation method thereof, and relates to the technical field of biological medicines. Polysaccharide is modified on the surface of a probiotic spore through an amido bond to load a first-line drug for treating IBD, and the outermost layer is chelated with metal ions to form an inflammation-targeted drug-loaded microgel precursor. Compared with the traditional hydrogel, the system has the advantages that the protection effect of the system on the medicine is far better than that of the traditional hydrogel, benefited from the natural germination characteristic of the intestinal tract after spores are orally taken, the medicine-carrying microgel precursor forms the medicine-carrying microgel along with the shedding of the spore shell protein, and the medicine-carrying microgel targets the positively charged inflammatory intestinal epithelium part, so that the targeted controlled release of the medicine is realized, and the intestinal inflammation is reduced; meanwhile, the microgel simulates the effect of an intestinal physical barrier, an artificial mucus coating is formed to prevent harmful bacteria from invading to an intrinsic layer, and the microgel and the probiotics are combined to regulate intestinal flora, so that synergistic treatment of IBD is achieved on the whole.

The invention relates to the technical field of genetic engineering and aquaculture, in particular to genetic engineering recombinant bacillus subtilis as well as a construction method and application thereof in vibrio infection resistance. Specifically, according to the recombinant bacillus subtilis, bacillus subtilis WB800N is taken as host bacteria, pDG364 is taken as a carrier, spore capsid protein Cot B is taken as anchoring protein, and the recombinant bacillus subtilis Bs-pDG364-Cot B-IL-8 capable of anchoring and expressing sebastes schlegeli interleukin-8 on the spore surface is constructed. The recombinant bacillus subtilis BB8 constructed by the invention not only can obviously improve the growth performance of sebastes schlegeli and improve the health state of intestinal tracts, but also can enhance the oxidation resistance, the non-specific immune level and the vibrio infection resistance of the sebastes schlegeli, provides a new effective strain for development and application of sebastes schlegeli immunopotentiator, and has a wide application prospect. The method has a wide application prospect in sebastes schlegeli breeding industry.