Integrative extract method of multi-active ingredient in cordyceps militaris mycelium

A technology for Cordyceps sinensis and active ingredients is applied in the field of extracting active ingredients in Chinese herbal medicines, which can solve the problems of resource waste, low purity and high cost, and achieve the effects of avoiding waste, saving ethanol consumption and reducing production costs.

Inactive Publication Date: 2008-07-30
和润通(重庆)供应链管理有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many extraction methods of active ingredients in Cordyceps militaris, but almost all of them only focus on unilateral extraction methods such as cordycepin, cordyceps polysaccharide and cordycepic acid, or simply study the compound extraction of cordycepin and cordyceps polysaccharide, however For other ingredients, such as cordycepin, vitamins, pigments, etc., they are removed as impurities or ignored, which leads to insufficient utilization of resources and a huge waste of resources
The Chinese patent with application number 200610023578.4 provides a method for continuously extracting the effective components of the fruiting body of Cordyceps militaris, which involves the comprehensive extraction of sterols, Cordyceps polysaccharides and cordycepin, but it still has the following defects: the raw material is the fruiting body of Cordyceps militaris, so the cost Higher; the purification methods such as supercritical extraction of sterols, normal phase low-pressure silica gel chromatography and preparative reversed phase high performance liquid chromatography of cordycepin used in it have many operation steps and are too complicated, which are difficult for ordinary small and medium-sized enterprises to realize; The sterols and Cordyceps polysaccharides prepared by it are not further purified, and the purity is not high

Method used

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  • Integrative extract method of multi-active ingredient in cordyceps militaris mycelium
  • Integrative extract method of multi-active ingredient in cordyceps militaris mycelium

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Extraction of Cordyceps sterol

[0039] Pulverization: Take 1000 grams of fresh Cordyceps militaris solid cultured mycelium and place it in a 50°C oven to dry to constant weight, and use a pulverizer to pulverize the dried mycelium;

[0040] Extraction: add 1000 ml of n-butane to 100 g of crushed mycelium, extract at 40°C for 2 hours, filter to obtain the filtrate, repeat the extraction 3 times, and use the residue for later use;

[0041] Concentration: Concentrate the filtrate under normal pressure at 40°C until there is no n-butane smell to obtain a concentrate, and recover n-butane;

[0042] Saponification: Add 2 times the volume of 10% sodium hydroxide solution to the concentrate at room temperature, mix well, let stand for 2 hours, remove the saponifiable matter in the lower layer to obtain unsaponifiable liquid, repeat twice;

[0043] Extraction: Add n-butane with 3 times the volume of unsaponifiable liquid, mix well, let stand for 2 hours, extract, take n-bu...

Embodiment 2

[0059] (1) Extraction of Cordyceps sterol

[0060] Pulverization: take 12000 grams of fresh mycelium cultured by liquid fermentation of Cordyceps militaris, place it in a 65°C oven, dry it to constant weight, and pulverize it with a pulverizer;

[0061] Extraction: Accurately weigh 1,000 grams of crushed mycelia and add 8 liters of n-hexane, extract at 65°C for 3 hours, filter to obtain the filtrate, repeat the extraction twice, and use the residue for later use;

[0062] Concentration: Concentrate the extract filtrate under reduced pressure at 50°C until there is no n-hexane smell to obtain a concentrated solution;

[0063] Saponification: add 10% sodium hydroxide solution of 3 times the volume of the concentrated solution to the above concentrated solution at room temperature, mix well, let stand for 4 hours, remove the saponifiable matter in the lower layer to obtain unsaponifiable solution, and repeat saponification twice;

[0064] Extraction: add 3 times the volume of un...

Embodiment 3

[0080] (1) Extraction of Cordyceps sterol

[0081] Pulverization: Take 9000 grams of leftovers after harvesting the fruiting bodies of Cordyceps militaris, place them in a 65°C oven, dry them to constant weight, and pulverize them with a pulverizer;

[0082] Extraction: Weigh 1000 g of crushed leftovers, add 8 liters of petroleum ether, extract at 65°C for 3 hours, filter to obtain the filtrate, repeat the extraction twice, and use the residue for later use;

[0083] Concentration: Concentrate the extracted filtrate under reduced pressure at 50°C until there is no petroleum ether smell to obtain a concentrated solution;

[0084] Saponification: add 10% sodium hydroxide solution of 3 times the volume of the concentrated solution to the above concentrated solution at room temperature, mix well, let stand for 4 hours, remove the saponifiable matter in the lower layer to obtain unsaponifiable solution, and repeat saponification twice;

[0085] Extraction: Add petroleum ether with...

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Abstract

The invention provides a comprehensive method of extracting various effective components from cordyceps militaris. The dry and crushed cordyceps militaris is extracted and filtered with organic solvent to get filtrate and leftover; the filtrate is condensed to recycling the solvent, and the condensed liquor after being saponified, extracted and dried can present aweto sterols; the leftover is extracted and filtered, and the sediment and supernatant are obtained after condensing, alcohol sinking and centrifugating the filtrate; cordycepin polysaccharide is obtained through operations such as dissolving the sediment into water and removing the albumen; the supernatant is processed by methods such as ion-exchange resin column and crystallization to obtain cordycepin. The method is material saving, with high purity of the extracts, low cost and simple operation, thus realizing the comprehensive extraction of various effective components from cordyceps militaris.

Description

technical field [0001] The invention relates to a method for extracting active ingredients in Chinese herbal medicines, in particular to a method for comprehensively extracting multiple active ingredients in the mycelia of Cordyceps militaris. Background technique [0002] Cordyceps is a class of edible and medicinal fungi with significant health benefits, the most famous of which is Cordyceps sinensis. Due to the special habitat and specific host of Cordyceps sinensis, it is very difficult to cultivate artificially, and the resource damage caused by excessive harvesting and digging makes the price of Cordyceps sinensis seriously deviate from its own value. Cordyceps militaris is most likely to replace Cordyceps militaris in the genus Cordyceps, also known as Cordyceps militaris and Cordyceps militaris. Cordyceps militaris is a close relative of Cordyceps sinensis and a model species of the genus Cordyceps. Cordyceps militaris is an excellent medicinal material and a substi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61P11/00A61P35/00C07J9/00C08B37/00C07H1/08
Inventor 毛先兵刘洪波朱华李叶伟
Owner 和润通(重庆)供应链管理有限责任公司
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