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Method for extracting melanin from cordyceps militeris fungus fermentation broth

A technology for Cordyceps sinensis fungus and Cordyceps sinensis, which is applied in the directions of microorganism-based methods, fermentation, chemical instruments and methods, etc., can solve the problems that the fermentation liquid cannot be well developed and utilized.

Inactive Publication Date: 2010-06-30
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the current shortage of natural Cordyceps sinensis resources, many scientific research institutes across the country have carried out research on the submerged fermentation of Cordyceps sinensis, and some have entered industrial production, but the fermented fermentation liquid has not been well developed and utilized.

Method used

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  • Method for extracting melanin from cordyceps militeris fungus fermentation broth
  • Method for extracting melanin from cordyceps militeris fungus fermentation broth
  • Method for extracting melanin from cordyceps militeris fungus fermentation broth

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Embodiment 1

[0025] Embodiment 1, extracting and purifying melanin from Cordyceps sinensis fermentation broth

[0026] 1) Isolation and identification of strains

[0027] Isolation of the strain: The strain was isolated from the specimens of Cordyceps sinensis collected in Kangding County, Sichuan Province.

[0028] The separation method is as follows: the collected fresh Cordyceps sinensis specimens are brushed off the soil and plant fragments on the surface, after the surface is disinfected with 75% ethanol, a small piece of tissue is taken and cultured on the culture medium. See [Yao Yijian, Dong Caihong, Wang Bo, Xie Xueqin (2005) A cultivation method of Cordyceps sinensis and its special medium. Patent No. ZL200510137457.8].

[0029] Strain identification:

[0030] Morphological identification: On the culture medium (ZL200510137457.8), the raised surface of the colony is uneven, and the back of the culture medium is obviously brown. Mycelium is colorless, septate, branched, 2.2-4....

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Abstract

The invention discloses a method for extracting melanin from cordyceps militeris fungus fermentation broth. The method includes the following steps: mycelium is separated from the cordyceps militeris fungus fermentation broth, and the fermentation broth is acidized to obtain melanin crude extract after the sediment is collected. The melanin crude extract is hydrolyzed by hydrochloric acid to eliminate protein impurities, and is repeatedly scrubbed by organic solvent and dissolved by alkaline solution, and is acidized to form sediment for purification, and then the chloride ions are eliminatedby deionized water through repeated scrubbing, finally the cordyceps militeris melanin end product is obtained after drying. The melanin prepared by the invention has similar properties with melanin CAS: 8049-97-6 sold by Sigma Corporation. Both two types of melanin can dissolve in alkaline solution, cannot dissolve in organic solvents, such as water, ethanol, acetone, chloroform and hexane, completely become sediment when pH value is less than 3 and can fade under the effect of strong oxidizer; the ultraviolet visible light area scan map includes a curvature with reducing absorbance along with the increasing of wavelength, and strong absorbing bands are respectively near 340cm-1 and 1602cm-1 in the infrared spectrum.

Description

technical field [0001] The invention relates to a method for extracting melanin from the fermentation liquid of Cordyceps sinensis. Background technique [0002] Melanin (Melanin) is a brown to black pigment widely present in animals, plants and microorganisms. It is a phenolic polymer with a relatively complex structure, which can improve the survival and competitiveness of organisms (Alois and Michael, Annual Review of Phytopathology 1986, 24:411-451). It is gradually evolved by organisms to resist the damage of ultraviolet rays and ionizing radiation, and can be regarded as a barrier used by organisms to protect themselves. The application potential of melanin is huge. It not only has anti-oxidation and anti-radiation effects, but also has immune promotion (Sava et al., Food Research International, 2001, 34(4): 337-343), anti-cancer (El-Obeid et al. , Phytomedicine, 2006, 13(5): 324-333), inhibiting virus replication (Montefiori and Zhou, Antiviral Research, 1991, 15(1)...

Claims

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Application Information

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IPC IPC(8): C09B61/00C07G99/00C12P7/00C12N1/14C12R1/645
Inventor 姚一建董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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