Culture medium for producing winter worm summer herb mycelium
A Cordyceps sinensis hyphae and culture medium technology, which is applied in the direction of fungi, etc., can solve the problems that the quality of solid-free medium and liquid medium is difficult to control, and is not suitable for industrial production, so as to improve product quality, not easily degrade, and ensure passage Effect
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Embodiment 1
[0019] The bacteria were inoculated on the slant, and the medium formula was 1.5% glucose, 1.0% corn flour, 0.5% dextrin, 1.5% bran, 0.5% yeast powder, 1.5% silkworm chrysalis powder, 1.0% peptone; 0.01% magnesium sulfate, phosphoric acid Potassium dihydrogen 0.02%; agar powder 0.8%, the rest is water, cultured at 12°C for 25 days; 1.0% bean cake powder, 0.5% yeast extract; 0.01% magnesium sulfate, 0.02% potassium dihydrogen phosphate, and the rest is water, placed on a shaker, cultured at 14°C for 8 days; then inoculated in a primary fermentation tank, cultured at 14°C 8 days, and then expand with 10 times the amount of medium, and ferment step by step until the 30-ton tank. Solid-liquid separation is carried out after leaving the tank, and the solid is dried and crushed.
Embodiment 2
[0020] Embodiment 2: the formula of fermentation medium is: glucose 1.5%, corn steep liquor 1.0%, molasses 1.5%; Silkworm chrysalis powder 1.0%, 2.0%, yeast extract 1.0%; Manganese sulfate 0.0125%, potassium chloride 0.01%, all the other are Water, the ventilation rate is 5:1. The slant culture medium and all the other steps and conditions are the same as in Example 1.
Embodiment 3
[0021] Example 3: inoculate the strains on the slope, the medium formula is: 1.0% glucose, 2.0% corn flour, 1.0% potato juice, 1.0% dextrin; 1.5% bran, 0.1% yeast powder, 3.0% silkworm chrysalis powder; Magnesium sulfate 0.01%, potassium chloride 0.01%; agar powder 0.8%, the rest is water, the fermentation medium and other steps and conditions are the same as in Example 1.
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