Method for producing Hirsutella sinensis

A technology of Trichospora chinensis and a production method, which is applied in the production field of a new strain of Trichospermum chinensis in China, can solve the problems of environmental pollution and flammability caused by the discharge of artificial cordyceps sinensis fermentation waste liquid, and achieve enhanced controllability and increased yield. , the effect of reducing environmental pollution

Active Publication Date: 2011-10-19
杭州柯氏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are also many deficiencies. People tend to get angry after using artificial Cordyceps fermented products, and a small number of gastrointestinal adverse reactions occur, while natural Cordyceps is warm and mild, and almost rarely o...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of production method of Mortierella chinensis is carried out by following steps:

[0038] (1) Incline culture of semi-solid bacteria:

[0039] Dissolve the medium in boiling water, adjust the pH to 7.2, and sterilize at 121°C for 30 minutes to make a slant medium; after cooling, put it in an incubator at 28°C and cultivate it for 3 days. After confirming that there is no bacterial contamination, it is imported into China Mortierella species, evenly coated; controlled temperature 17 ~ 18 ℃ culture, culture time 40 days; then, put into 3 ℃ refrigerator for storage; wherein the composition of the slant medium is by weight percentage: defatted silkworm chrysalis powder 2.1%, corn Flour 1.5%, Bran 1.5%, Glucose 2%, KH 2 PO 4 0.02%, MgSO 4 0.01%, agar 1.8%, the rest is water; the inoculum amount is 15%;

[0040] (2) Shake flask cultivation of liquid bacteria:

[0041] Inoculate the strains cultivated on the slant into shake flasks with liquid medium, and culture t...

Embodiment 2

[0055] The same method as the embodiment is implemented through the following steps

[0056] (1) Incline culture of semi-solid bacteria:

[0057] Dissolve the medium in boiling water, adjust the pH to 7.2, and sterilize at 121°C for 30 minutes to make a slant medium; after cooling, put it in an incubator at 28°C and cultivate it for 3 days. After confirming that there is no bacterial contamination, it is imported into China Trichosporum species, evenly coated; controlled temperature 18 ~ 19 ° C culture, culture time 45 days; then, put into 4 ° C refrigerator for storage; wherein the composition of the slant medium is by weight percentage: defatted silkworm chrysalis powder 2.9%, corn Flour 1.5%, Bran 4.5%, Glucose 2%, KH 2 PO 4 0.02%, MgSO 4 0.01%, agar 1.8%, and the rest are water; wherein the inoculation amount is 15%; the insertion of Mortierella chinensis species is at 18°C, the inoculum amount is 10%, and it is flame-protected aseptic inoculation; wherein the defatted ...

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Abstract

The invention relates to a method for producing a microbial strain, in particular to a method for producing a new variety of Hirsutella sinensis. In the invention, Hirsutella sinensis screened according to cell dynamic information acquired by rat macrophage culture is subjected to multistage culture and fermentation, then hyphae is filtered by a membrane with a molecular weight between 300 and 4,000 by a solid-liquid separation technique, the bacterial solution is dried, and thus, health-care food and medicines, which can improve an immune function, are manufactured. When the method is used, the instability of industrial Cordyceps sinensis strain is realized, digital standard control is applied in a whole process from strain screening to culture and fermentation to solid-liquid membrane treatment, and the standard production process of industrial Cordyceps sinensis is realized actually; and with an accurate screening process, high production speed, pure production process and low production cost, the method can be widely accepted by people.

Description

technical field [0001] The invention relates to a production method of microbial strains, in particular to a production method of a new strain of Mortierella chinensis. technical background [0002] Cordyceps sinensis is a kind of precious Chinese medicinal material in China. Wild resources are extremely collected. With the increasing market demand, the domestic and foreign markets are in short supply and the price is high. Therefore, using the Cordyceps sinensis strain isolated from natural Cordyceps sinensis—— Trichosporum chinensis is cultivated through submerged fermentation, and the fermentation product is filtered and dried to make finished products. Because of its stable quality and reasonable price, it is becoming more and more popular. Using Mortierella chinensis as the strain to ferment and produce products (such as Bailing capsules), not only effectively alleviates the contradiction between supply and demand in the Cordyceps sinensis market, delays the deteriorati...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 徐洋翟云燕柯越海
Owner 杭州柯氏生物科技有限公司
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