Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers

A technology of artificial Cordyceps and silkworm chrysalis, which is applied in the fields of botany equipment and methods, horticulture, application, etc., and can solve problems such as the inability to produce organic silkworm chrysalis Cordyceps

Inactive Publication Date: 2014-04-02
凌中鑫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this time, most of the silkworm chrysalis used are those produced after the purchase of traditional cocoon silk, which contains chemicals such as strong alkali, and are not organic silkworm chrysalis. Using this as a host cannot produce organic silkworm chrysalis Cordyceps.

Method used

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Embodiment 1

[0044] A method for cultivating artificial Cordyceps using live silkworm chrysalis as a culture medium.

[0045] The specific technical scheme is as follows:

[0046] (1) Screening of Cordyceps strains

[0047] 1. Production of strains

[0048]Select high-yield, high-quality, early-maturing wild fresh fruiting bodies of Cordyceps sinensis native to the Qinghai-Tibet Plateau, disinfect the surface with 75% alcohol, wash with sterile water several times (generally 5-6 times), and remove the worms in Cordyceps sinensis , and the rest were cultured in culture medium. Purify after the mycelia cover the plate. Several mother species were obtained after repeated purification.

[0049] After expanding the cultivation of the mother species, place it on an organic medium with silkworm chrysalis powder (remove the epidermal wax) as a nitrogen source, and cultivate it at 18°C-25°C for 20-30d, observe the growth situation, and if there is any contamination by bacteria, respond immediat...

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PUM

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Abstract

A method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers is used for inoculating bacterial suspension prepared by using wild natural cordyceps sinensis bacterial strains native to the Tibet plateau on live silkworm pupas and artificially culturing the live silkworm pupas into silkworm pupa cordyceps sinensis. The method comprises the following steps of (1) screening of cordyceps sinensis bacterial strains, (2) preparation of the silkworm pupas, (3) preparation of bacterial suspension, (4) preparation of silkworm pupas before inoculation, (5) inoculation, (6) culturing of seed seats, and (7) collection and storage of silkworm pupa cordyceps sinensis. According to the method, silkworm pupas of domesticated silkworms serve as hosts, the cordyceps sinensis bacterial strain suspension is inoculated in the silkworm pupas artificially, and then the silkworm pupas are cultured into the silkworm pupa cordyceps sinensis. Inoculation rate and petrification rate after inoculation are above 96%, and the yield of seed entities is above 92%. The period from inoculation to mature of the seed entities takes 36-44 days. The chemical composition of the silkworm pupa cordyceps sinensis is the same with that of cordyceps sinensis basically, and the active ingredients such as cordycepin and cordycepic acids of the silkworm pupa cordyceps sinensis are 3.2-3.9 times the active ingredients of the cordyceps sinensis.

Description

technical field [0001] The invention belongs to the technical field of medicinal fungus cultivation, and relates to a method for cultivating artificial Chinese caterpillar fungus with silkworm chrysalis as a carrier, in particular to a method for preparing a bacterial suspension from a wild natural Cordyceps sinensis strain native to the Qinghai-Tibet Plateau and inoculating it on live silkworm chrysalis for artificial cultivation The cultivation method of artificial cordyceps. Background technique [0002] With the improvement of people's living standards and the emphasis on health, Cordyceps sinensis and its subsidiary products have become a rapidly developing industry. Cordyceps sinensis is listed as a national second-class protected species, and local governments have also introduced policies to prohibit and limit digging, but the digging of Cordyceps sinensis has not been curbed. Cordyceps sinensis is a kind of precious traditional Chinese medicine in my country. Becau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 凌中鑫
Owner 凌中鑫
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