Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers

Abstract

A method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers is used for inoculating bacterial suspension prepared by using wild natural cordyceps sinensis bacterial strains native to the Tibet plateau on live silkworm pupas and artificially culturing the live silkworm pupas into silkworm pupa cordyceps sinensis. The method comprises the following steps of (1) screening of cordyceps sinensis bacterial strains, (2) preparation of the silkworm pupas, (3) preparation of bacterial suspension, (4) preparation of silkworm pupas before inoculation, (5) inoculation, (6) culturing of seed seats, and (7) collection and storage of silkworm pupa cordyceps sinensis. According to the method, silkworm pupas of domesticated silkworms serve as hosts, the cordyceps sinensis bacterial strain suspension is inoculated in the silkworm pupas artificially, and then the silkworm pupas are cultured into the silkworm pupa cordyceps sinensis. Inoculation rate and petrification rate after inoculation are above 96%, and the yield of seed entities is above 92%. The period from inoculation to mature of the seed entities takes 36-44 days. The chemical composition of the silkworm pupa cordyceps sinensis is the same with that of cordyceps sinensis basically, and the active ingredients such as cordycepin and cordycepic acids of the silkworm pupa cordyceps sinensis are 3.2-3.9 times the active ingredients of the cordyceps sinensis.

Description

technical field [0001] The invention belongs to the technical field of medicinal fungus cultivation, and relates to a method for cultivating artificial Chinese caterpillar fungus with silkworm chrysalis as a carrier, in particular to a method for preparing a bacterial suspension from a wild natural Cordyceps sinensis strain native to the Qinghai-Tibet Plateau and inoculating it on live silkworm chrysalis for artificial cultivation The cultivation method of artificial cordyceps. Background technique [0002] With the improvement of people's living standards and the emphasis on health, Cordyceps sinensis and its subsidiary products have become a rapidly developing industry. Cordyceps sinensis is listed as a national second-class protected species, and local governments have also introduced policies to prohibit and limit digging, but the digging of Cordyceps sinensis has not been curbed. Cordyceps sinensis is a kind of precious traditional Chinese medicine in my country. Becau...

AI Technical Summary

Problems solved by technology

At this time, most of the silkworm chrysalis used are those produced after the purchase of traditional cocoon silk, which contains chemicals such as strong alkali, and are not organic silkworm chrysalis. Using this as a host cannot produce organic silkworm chrysalis Cordyceps.