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Chinese caterpillar fungus bionic culturing method

A Cordyceps sinensis fungus and culture method technology, applied in the field of microbial culture in the biological field, can solve the problems of large-scale production that is difficult to industrialize, gene cloning is impossible to talk about, and commercial production cannot be achieved, so as to achieve sufficient oxygen and reliable pure culture , Conducive to the effect of large-scale production

Active Publication Date: 2006-05-03
江西荣善绿色中药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are three ways to partially solve this problem: the first is to artificially cultivate the fruiting bodies. Our country has spent a lot of manpower and material resources. Although the basic technology of artificial cultivation has been mastered, it is still unable to commercialize production; the second is to use molecular biotechnology. Clone the genes encoding these functions and express these functions in other organisms. However, the material basis of most of the functions of Cordyceps sinensis has not been studied clearly, and gene cloning is out of the question; the third is to use anamorphic strains for mycelium Fermentation culture, and then extract functional components from mycelia, this may be a more realistic and simple development approach at present (Mo Minghe et al., Mycosystem 20(4): 482-485, 2001)
The cultivation cycle of fermenter, shaker and other equipment is long and consumes a lot of energy, and the cost of investment in fermenter, shaker and other equipment itself is also high. Therefore, the products produced by this method are expensive and unacceptable by the market.
Difficult to use for industrial mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Inoculate the aseptic Cordyceps sinensis mycelium in soybean powder 6g / L, peptone 4g / L, glucose 100g / L, yeast extract 0.4g / L, K 2 HPO 4 0.8g / L, MgSO 4 0.4g / L, pH 6 sterile liquid culture medium, placed statically on a culture rack, the culture temperature was 18°C, the pressure was normal pressure, and the light intensity was indoor natural light.

[0037] The mycelium was harvested after six months.

Embodiment 2

[0039] The culture procedures were all carried out as in Example 1, except that the culture container was placed on a magnetic stirrer and stirred for 0.5 hours (18° C.) every month during the culture period to break the mycelia.

Embodiment 3

[0041] 1. Isolation of Trichosporum sinensis from Cordyceps sinensis

[0042] (1) Collection of Cordyceps strains

[0043] In July 2002, in Kangding, Sichuan Province, with the help of local drug farmers, a number of Cordyceps sinensis complete fungus complexes were excavated from Yajiageng with strong and plump fruiting bodies and obvious fertile parts, and put them in an incubator at 15°C for maintenance. Humidity, the soil should not agglomerate after light kneading by hand.

[0044] (2) Ascospore collection and germination

[0045] Remove impurities on the surface of the fruiting body, rinse with sterile distilled water, disinfect the surface with 75% alcohol, place it in an incubator at 15°C, and record and observe every day. When the ascus starts to eject the ascospores, put the fruiting bodies that start to eject the spores into a penicillin vial filled with sterilized physiological saline, fully elute the ascospores, and place the eluted ascospores in an incubator at...

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Abstract

The invention relates to a bionic culture method for winter worm summer herb mycelium, which comprises: separating and pure culturing monospore, preparing liquid medium, inoculating the pure culture bacterium of mycelium, static culturing the superficial-layer liquid for six months; harvesting the mycelium; wherein, breaking the mycelium into monocellular or multicellular mycelium every month during the culture process. This invention needs no fermentor or other dear devices, and reduces cost more than 50% for 1g dry mycelium.

Description

technical field [0001] The invention relates to a bionic cultivation method of Cordyceps sinensis fungus, which belongs to the microorganism cultivation method in the field of biology. Background technique [0002] Cordyceps sinensis (Berk.) Sacc., belongs to Ascomycetes, Hypocreales, Ergotaceae, Cordyceps, alias Cordyceps, is a parasitic fungus on the larvae of Phosphoptera bat moth Hepialus spp. A rare medicinal fungus. The ancient folks in our country had a certain understanding of the medicinal value of Cordyceps sinensis. Cordyceps is sweet in taste and flat in nature, which can protect the lungs and kidneys, nourish the essence, resolve phlegm and relieve cough. Houjiuwei, hemoptysis and hematemesis, chronic nephritis, etc. have very good effects. Regular consumption can regulate immune function and enhance human immunity. It is precisely because Cordyceps sinensis has so many medicinal functions and origins of medicinal history, this wild resource is on the verge o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 田向荣田绍进刘彦威苏敬良韩博
Owner 江西荣善绿色中药有限公司
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