Corayceps militaris polysaccharide two-stage fermentation synthesis process

A technology for the synthesis of Cordyceps militaris, which is applied in the field of synthesizing polysaccharides from Cordyceps militaris using a biological two-stage fermentation method, can solve the problems of low yield of biologically active substances, and achieve the effects of increased yield, significant benefits, and simple operation
CN102154407AInactive Publication Date: 2011-08-17HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the microorganism field, particularly relates to a corayceps militaris polysaccharide two-stage fermentation synthesis process; the process comprises the following steps: corayceps militaris strains are vaccinated on a potato slant culture medium, are cultured at the constant temperature and then are put in a conical flash containing a seed culture medium for shaking culture; then the corayceps militaris strains are put in a fermentation tank containing a fermentation medium, the fermentation process is as follows: the corayceps militaris is cultured at constant temperature for four to five days in ventilating and stirring condition, which is mainly used for providing a culture condition suitable for growing of corayceps militaris thallus so that the corayceps militaris grows rapidly; then the corayceps militaris is cultured for two to three days at the constant temperature in the condition of ventilation and slightly stirring, a culture solution is centrifuged, and ethanol in two times of volume of is added into an obtained liquid supernatant which is mixed and put up for the night at the temperature of 4 DEG C; and precipitates obtained through secondary centrifugation is dried so as to obtain the corayceps militaris. The process is reasonable in design and simple in operation, is suitable for large-scale industrial production and can solve the situation of drug source shortage and has obvious benefits.
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Description

technical field

[0001] The invention relates to a process for artificially cultivating and synthesizing polysaccharides from Cordyceps militaris, in particular to a method for synthesizing polysaccharides from Cordyceps militaris by a biological two-stage fermentation method. Background technique

[0002] Cordyceps militaris [Cordyceps militaris] (commonly known as Cordyceps militaris) is a complex of insects and fungi formed by the larvae of the Lepidoptera bat mothidae after being parasitized by Chinese hairy sporophytes. It is listed as one of the "Three Treasures of Traditional Chinese Medicine" in China. It contains a variety of active ingredients (such as Cordyceps mycelium, polysaccharides, cordycepin, etc.), which have the functions of enhancing immunity, anti-tumor, anti-oxidation and lowering blood sugar, and the market demand is huge.

[0003] However, the growth cycle of wild Cordyceps sinensis is too long, and due to the indiscriminate digging and harvesting of...

Claims

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