Method of whole manual culture for chinese caterpillar fungus

A technology of artificial cultivation of Cordyceps sinensis, which is applied in the direction of medical formulas, medical preparations containing active ingredients, fungi, etc., can solve the problems of not being able to obtain Cordyceps sinensis, and achieve long growth time, stable product quality, and high infection success rate Effect

Active Publication Date: 2005-01-05
张雪峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Some biological experts use liquid submerged fermentation to solve the market demand for Cordyceps sinensis. Because the application of liquid submerged fermentation can only produce the mycelium of Cordyceps sinensis, it can only be processed into a preparation o

Method used

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  • Method of whole manual culture for chinese caterpillar fungus
  • Method of whole manual culture for chinese caterpillar fungus
  • Method of whole manual culture for chinese caterpillar fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 The full artificial cultivation method of Cordyceps sinensis of the present invention

[0043] 1.1 Collection of samples

[0044] Cordyceps samples were collected from alpine shrub meadows at an altitude of 3,500 to 5,000 meters in Ganzi Prefecture, Sichuan.

[0045] 1.2 Separation of strains

[0046] 1.2.1 Basic medium for separation: potato, glucose, agar, plus peptone (referred to as PDA + peptone) and potato, glucose, agar, plus silkworm chrysalis juice.

[0047] Its weight proportion: potato 200g, glucose 20g, agar 20g, peptone 10g, water 1000ml.

[0048] 1.2.2 Separation method

[0049] Sclerotia (worm body) and sub-seat separation: Sclerotia and sub-seats are surface-sterilized with 0.1% mercury chloride solution, washed with sterile water, cut into small sections or thin slices, placed on a culture dish with medium, and kept at 8- Incubate at 18°C.

[0050] Separation of ascospores: take the head of the ascospore, disinfect the surface with 0.1% ...

Embodiment 2

[0093] Embodiment 2 The quality control of the Cordyceps sinensis cultivated by the method of the present invention

[0094] Natural Cordyceps %

All Artificial Cordyceps

%

Cordyceps sinensis

Insect body part

seat part

ASP. Aspartic Acid

1.646

1.658

2.201

3.449

THR.Threonine

0.911

0.994

1.269

1.751

SER. Serine

1.080

1.029

1.609

1.424

GLU. Glutamic acid

2.360

2.836

3.498

4.172

GLY. Glycine

0.954

0.998

1.261

1.886

ALA.Alanine

1.140

1.159

1.451

2.698

CYS. Cystine

0.381

0.377

0.437

0.333

VAL. Valine

0.719

0.880

0.910

1.634

MET.Methionine

0.285

0.171

0.338

0.543

ILE. Isoleucine

2.280

...

Embodiment 3

[0100] Embodiment 3 Cordyceps sinensis preparation cultivated by the method of the present invention

[0101] Get 300g of Cordyceps sinensis cultivated by the method of the present invention, directly powder it, pack into capsules, and make capsules, and each capsule contains 0.3g of Cordyceps sinensis medicinal material.

[0102] Prove beneficial effect of the present invention by pharmacodynamic test below.

[0103] The fully artificially cultivated Cordyceps sinensis has a calming effect on the central nervous system; it can significantly expand the bronchus on the respiratory system, and can significantly enhance the effect of adrenaline, and it also has the effect of eliminating phlegm, relieving asthma and preventing emphysema.

[0104] For the cardiovascular system, it can reduce myocardial oxygen consumption, improve myocardial ischemia, anti-platelet aggregation, and anti-arrhythmia.

[0105] It has androgen-like effects on the endocrine system.

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Abstract

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

Description

technical field [0001] The invention provides a fully artificial culture method for Cordyceps sinensis, which belongs to the field of biology. Background technique [0002] Cordyceps sinensis is a rare fungus Chinese herbal medicine. It is a sexual form produced by the larvae of bat moths in China that are infected by hair spores and only grow in alpine shrub meadows at 3500-5000 meters. Dig out when the spores are unearthed and the spores are not dispersed, remove the fibrous mycelium wrapped around the insect body, and dry in the sun or at low temperature. [0003] The bat moth larvae, the host of Cordyceps sinensis, live in the soil. After being invaded by Trichosporum sinensis, they continue to grow and multiply in the larvae with the circulation of body fluids, filling the entire larvae to form sclerotia, and finally produce a subsea from the head of the larvae. [0004] Cordyceps sinensis is mainly distributed in the plateaus above 3,500 meters in the southwest and no...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 俞永信
Owner 张雪峰
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