Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae

A technology of Chinese hairy sporophytes and bat moth larvae, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of high cost, long cycle, low infection rate, etc., and achieve low cost, short cycle, and good application foreground effect

Inactive Publication Date: 2012-06-20
许继宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two methods are highly random, the infection rate is very low, the cycle is long, and the cost is high, which is not meaningful for commercial or artificial large-scale cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, carry out according to the following steps:

[0033] (1) Make sterile infection stock solution. The following raw materials are mixed by weight: silkworm chrysalis 31%, potato 6%, magnesium sulfate 0.009%, potassium dihydrogen phosphate 0.045%, and water as the balance. After mixing, boil, filter, and sterilize to obtain an aseptic infection stock solution.

[0034] (2) Production of infection source bacteria liquid: use the pure cultured Trichosporum sinensis obtained by isolation and culture as the source of infection bacteria, use an inoculation needle on a clean workbench, separate from the culture bottle of pure cultured Trichosporum sinensis without culture 10 needles of pure mycelia based on the base were added to 5 ml of the sterile infection stock solution obtained in the previous step, and ground in a bowl for 1 minute to prepare the infection source bacterial solution. The purely cultured Mortierella sinensis obtained by the isolation and cultu...

Embodiment 2

[0036] Embodiment 2, carry out according to the following steps:

[0037] (1) Make sterile infection stock solution. The raw materials in the following weight ratio: 28% silkworm chrysalis, 7% potato, 0.01% magnesium sulfate, 0.037% potassium dihydrogen phosphate, and water as the balance, boiled after mixing, filtered, concentrated, constant volume, sterile after sterilization Infection stock.

[0038] (2) Make the infection source bacteria liquid: use the pure cultured Trichosporum sinensis obtained by isolation and culture as the source of the infectious bacteria, use the inoculation needle on the clean workbench, and pick off the pure cultured Trichosia sinensis from the wall of the culture bottle without inoculation. Add 15 needles of pure mycelium with culture medium to each 5ml of the sterile infection stock solution obtained in the previous step, and grind it in a bowl for 2 minutes to prepare the infection source bacterial solution.

[0039] (3) Take 20 collected ba...

Embodiment 3

[0040] Embodiment 3, carry out according to the following steps:

[0041] (1) Make sterile infection stock solution. The raw materials in the following weight ratio: 34% of silkworm chrysalis, 5% of potato, 0.008% of magnesium sulfate, 0.05% of potassium dihydrogen phosphate, and water as the balance, boiled after mixing, filtered, concentrated, constant volume, sterile after sterilization Infection stock.

[0042] (2) Production of infection source bacteria liquid: use the pure cultured Trichosporum sinensis obtained by isolation and culture as the source of infection bacteria, use an inoculation needle on a clean workbench, separate from the culture bottle of pure cultured Trichosporum sinensis without culture Add 14 needles of pure mycelia based on the sterile infection stock solution obtained in the previous step to every 5ml, and grind it in a bowl for 1.5 minutes to prepare the infection source bacterial solution.

[0043] (3) Take 50 collected bat moth larvae, use a 4...

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PUM

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Abstract

The invention relates to a method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae, belonging to the technical field of artificial culture of the cordyceps sinensis. The method comprises the steps of: mixing the following components in percentage by weight: 28-34% of bombyx mori pupae, 5-7% of potatoes, 0.008-0.01% of magnesium sulfate, 0.035-0.05% of potassium dihydrogen phosphate and the balance of water, boiling, filtering and sterilizing to prepare an aseptic infection stock solution; taking the hirsutella sinensis as an infection bacteria source, picking pure mycelia through inoculating needles, placing the pure mycelia into the aseptic infection stock solution according to the proportion that the hirsutella sinensis picked by 10-15 needles are placed into every 5ml of the aseptic infection stock solution, grinding and then preparing an infection source bacterial solution; and sucking the infection source bacterial solution through a syringe, inserting a needle head from the excretory opening of each hepialus larva to press 0.3-0.5ml of the infection source bacterial solution into the intestinal tract of the larva, then placing the larvae in the shade and culturing under the conditions that the temperature is 17-21 DEG C and the relative humidity is 78-85% for more than 15 days. The method disclosed by the invention has the advantages of infection rate of up to above 90 percent, short period, low cost and great application prospects for large-scale culture of the cordyceps sinensis.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of Cordyceps sinensis, in particular to a method for artificially infecting Trichosporum sinensis by endogenous pressurization of bat moth larvae during the cultivation process of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a very valuable medicinal fungus, which is the fungal infection body of bat moth larvae formed after the insect bat moth larvae are infected with Hirsutalla sinensis. The larvae of the bat moth infected by the fungus died because the body was covered with the fungus after infection, and then under special environmental conditions, the "grass" that grew from the dead larvae of the bat moth was the fruiting body of the fungus Trichosporum sinensis — Sub-seat. [0003] The fungus Mortierella sinensis belongs to the subphylum Ascomycotina, the class Pyrenomycetes, and the order Clavicipitales in taxonomy. There are also many literatu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 许继宏
Owner 许继宏
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