Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae

Abstract

The invention relates to a method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae, belonging to the technical field of artificial culture of the cordyceps sinensis. The method comprises the steps of: mixing the following components in percentage by weight: 28-34% of bombyx mori pupae, 5-7% of potatoes, 0.008-0.01% of magnesium sulfate, 0.035-0.05% of potassium dihydrogen phosphate and the balance of water, boiling, filtering and sterilizing to prepare an aseptic infection stock solution; taking the hirsutella sinensis as an infection bacteria source, picking pure mycelia through inoculating needles, placing the pure mycelia into the aseptic infection stock solution according to the proportion that the hirsutella sinensis picked by 10-15 needles are placed into every 5ml of the aseptic infection stock solution, grinding and then preparing an infection source bacterial solution; and sucking the infection source bacterial solution through a syringe, inserting a needle head from the excretory opening of each hepialus larva to press 0.3-0.5ml of the infection source bacterial solution into the intestinal tract of the larva, then placing the larvae in the shade and culturing under the conditions that the temperature is 17-21 DEG C and the relative humidity is 78-85% for more than 15 days. The method disclosed by the invention has the advantages of infection rate of up to above 90 percent, short period, low cost and great application prospects for large-scale culture of the cordyceps sinensis.

Description

technical field

[0001] The invention belongs to the technical field of artificial cultivation of Cordyceps sinensis, in particular to a method for artificially infecting Trichosporum sinensis by endogenous pressurization of bat moth larvae during the cultivation process of Cordyceps sinensis. Background technique

[0002] Cordyceps sinensis is a very valuable medicinal fungus, which is the fungal infection body of bat moth larvae formed after the insect bat moth larvae are infected with Hirsutalla sinensis. The larvae of the bat moth infected by the fungus died because the body was covered with the fungus after infection, and then under special environmental conditions, the "grass" that grew from the dead larvae of the bat moth was the fruiting body of the fungus Trichosporum sinensis — Sub-seat.

[0003] The fungus Mortierella sinensis belongs to the subphylum Ascomycotina, the class Pyrenomycetes, and the order Clavicipitales in taxonomy. There are also many literatu...