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Method for evaluating toxicity of pesticides on bee larva by the aid of laboratory artificial-breeding bee larva

A bee larvae and laboratory technology is applied in evaluating the toxicity of pesticides to honeybee larvae, and the use of artificially reared honeybee larvae in the laboratory to evaluate the toxicity of pesticides to honeybee larvae can solve the problems of increased test cost, damage to tested larvae, time-consuming and labor-intensive, etc. Achieve strong practicability and scalability, accurate evaluation, and a wide range of applications

Active Publication Date: 2017-05-24
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operator needs to have good operating experience, and the transfer of larvae needs to be carried out every day. This process is often time-consuming and laborious. For example, when a batch of bee larvae is required to carry out the test, the cost of the test will be greatly increased, and the process of the test will be slowed down. The larvae cause certain damage, which affects the accuracy of later test results

Method used

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  • Method for evaluating toxicity of pesticides on bee larva by the aid of laboratory artificial-breeding bee larva
  • Method for evaluating toxicity of pesticides on bee larva by the aid of laboratory artificial-breeding bee larva

Examples

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Effect test

Embodiment 1

[0042] Embodiment 1: the method for artificially raising honeybee larvae in the laboratory of the present invention comprises the following steps:

[0043] (1) Preparation of bee larvae

[0044] During the spawning period of the queen bee, select three boxes of bee colonies that have not been treated for four weeks, are healthy, have known source and physiological state. Open the beehive cover and insert one empty spleen, fix the queen bee on the empty spleen with a queen partition to lay eggs, remove the queen partition after 24 hours to release the queen bee, and check the new oviposition situation. According to the fertility of the queen bee, the isolation time can be appropriately shortened to reduce the size and age differences among the larvae. The honeycomb containing bee eggs continues to remain in the beehive until hatching (the bee egg hatching period is generally 3 days).

[0045] (2) Laboratory rearing of bee larvae

[0046] The inserted honeycomb is taken out f...

Embodiment 2

[0064] Embodiment 2: the present invention utilizes the method for artificially raising bee larvae in the laboratory to evaluate the toxicity of pesticides to bee larvae, comprising the following steps:

[0065] (1) Preparation of bee larvae

[0066] During the spawning period of the queen bee, select three boxes of bee colonies that have not been treated for four weeks, are healthy, have known source and physiological state. Open the beehive cover and insert one empty spleen, fix the queen bee on the empty spleen with a queen partition to lay eggs, remove the queen partition after 24 hours to release the queen bee, and check the new oviposition situation. According to the fertility of the queen bee, the isolation time can be appropriately shortened to reduce the size and age differences among the larvae. The honeycomb containing bee eggs continues to remain in the beehive until hatching (the bee egg hatching period is generally 3 days).

[0067] (2) Laboratory rearing of be...

Embodiment 3

[0077] Using a method similar to Example 2, taking Italian honeybee larvae as the research object, and using imidacloprid, a typical neonicotinoid pesticide, as a representative, the toxicity research of pesticides on honeybee larvae was carried out. After the preparation of honeybee larvae was completed, on the first day of the experiment, 1-day-old late larvae were selected from three bee colonies, placed on a 48-well plate, fed with the same amount of basal diet, and adapted for three days (temperature 34±1°C) , Humidity 95%), health checks were carried out on the 4th day after the start of the test, abnormal, sick and injured larvae were removed, and then distributed to each treatment group. The experiment set up a blank control group (C1, always fed with basal diet), a solvent control group (C2, always fed with basal diet) and 5, 50, 500, 1000, 2000 and 3000mg·L -16 concentration treatment groups (add 1 μL of drug solution to the basal diet on the 4th, 5th and 6th days af...

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Abstract

The invention discloses a method for evaluating toxicity of pesticides on bee larva by the aid of laboratory artificial-breeding bee larva. The method includes steps of preparation of the bee larva, laboratory-breeding of the bee larva, preparation of tested pesticides, adding of the tested pesticides and the like and finally observation of the process that the larva complete defecation, spinning, pupation and eclosion. Through laboratory-breeding of the bee larva, research on the toxicity of the pesticides on the bee larva can be directly carried out in a laboratory without dependence on swarms; with the method, survival rate in breeding of the bee larva is quite high, and growth situations are basically identical; the method is labor saving, efficient and capable of obtaining the bee larva in batch with the growth states basically identical in a short time and evaluating effect of the pesticides on the bee larva visually, efficiently and accurately; meanwhile, many in-vivo experiments with the bee larva as objects can be carried out, high practicability and expansibility are achieved, and better materials and methods are provided for the relevant research and practical work.

Description

technical field [0001] The invention belongs to the field of ecotoxicity testing, and relates to a method for evaluating the toxicity of pesticides to bee larvae, and more specifically relates to a method for evaluating the toxicity of pesticides to bee larvae by artificially raising bee larvae in a laboratory. Background technique [0002] Bees play an important role in the pollination of many crops due to their advantages of high pollination efficiency, collection specificity and population sociality. However, the abuse of pesticides not only has a serious impact on the collection behavior and health of collecting bees, but also the pesticides polluted by pesticides in honey, pollen and pesticides accumulated in the honeycomb will also affect the growth and development of bee larvae. Mass death. The fewer the number of larvae in the bee colony, the smaller the possibility of developing a strong colony, the worse its collection ability, the ability to produce bee products ...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90
CPCA01K67/033
Inventor 谭丽超卜元卿程燕周军英单正军
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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