Inducible promoter capable of synchronously responding to induction of salicylic acid (SA) and jasmonic acid (JA)
A technology of promoter and jasmonic acid, which is applied in application, DNA/RNA fragments, DNA preparation, etc., can solve the problems of simultaneous resistance and simultaneous resistance to necrotrophic pathogens, etc., and achieve the effect of high induction activity
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[0034] Specific implementation examples
[0035]The following implementation examples further define the present invention. According to the above description and these implementation examples, those skilled in the art can ascertain the essential features of the present invention. Unless otherwise specified, what the present invention adopts is the prior art in the field of the present invention. The materials and reagents used in the implementation examples can be purchased from commercial sources unless otherwise specified.
[0036] 1. Obtaining the PR-1a starting sequence:
[0037] 1.1 Extraction of tobacco DNA:
[0038] About 100 mg of wild-type tobacco leaves were taken, and the tobacco genomic DNA was extracted according to the instruction manual using the new plant genomic DNA rapid extraction kit (Aidlab).
[0039] 1.2 Acquisition of PR-1a promoter:
[0040] Add HindⅢ to the 5' end, add a BamHI restriction site to the 3' end, design primers SEQ7 and SEQ8 respective...
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