An inducible promoter capable of simultaneously responding to salicylic acid sa and jasmonic acid ja induction and its application

Abstract

The invention provides an inducible promoter capable of synchronously responding to induction of salicylic acid (SA) and jasmonic acid (JA). The inducible promoter is a novel synthetic promoter obtained by inserting a JA responding element in a PR-1a promoter by using a nest type PCR amplification method with a tobacco PR-1a promoter as a basic skeleton, and is named as PR-1a-JA. An inducible activity analysis result shows that the PR-1a-JA can synchronously respond to the induction of SA and JA, has higher SA inducible activity, and can respond to the induction of pathogenic bacteria; responding parts are mainly concentrated in an infection part. The inducible promoter has the advantages of no background expression and no induction from abiotic stress such as high temperature, low temperature, high salt and mechanical injury.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an inducible promoter capable of simultaneously responding to the induction of salicylic acid SA and jasmonic acid JA and its application. technical background [0002] Since humans began to cultivate crops, plant diseases have been an important factor affecting crop production. Crop diseases not only affect the growth and development of crops, resulting in reduced yields or even crop failures, but also affect the quality of products and bring invisible harm to human health. my country is a large agricultural country, and plant diseases have always been an important reason for crop production reduction. The average annual crop loss due to diseases accounts for more than 10% of crop production. At present, the control of pests and diseases is still dominated by chemical pesticides, which not only causes serious pollution to the environment, but also easily makes pests and diseases re...

AI Technical Summary

Problems solved by technology

The immune response of plants to biotrophic type is related to SA signaling pathway, and the immune response to necrotrophic type is related to JA signaling pathway, and these two signaling pathways are mutually antagonistic. Therefore, in addition to the immune function of plants themselves, through genetic engineering It is difficult to obtain the characteristics of simultaneous resistance of plants to biotrophic and necrotrophic pathogens, and it is also difficult to achieve the purpose of simultaneous resistance to different vegetative growth stages of facultative trophic pathogens

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