Method for evaluating safety of vegetables and fruits by using zebra fish

Abstract

The invention provides a method for evaluating the safety of vegetables and fruits by using zebra fish. The method comprises the following steps of constructing a zebra fish toxicity evaluation model, pre-treating the vegetables and the fruits in a manner of enzymolysis first and then chemical extraction to obtain an extract of a detection product, treating the extract by using the zebra fish to obtain the toxicity evaluation data, and analyzing and evaluating the safety of the vegetables and the fruits. The method provided by the invention is high in detection accuracy, scientific and reasonable in detection method and short in modeling period, so that the detection cost is greatly reduced, and practical feasibility is provided for popularizing and popularizing the detection method to the broad public, enterprises and governments.

Description

technical field [0001] The invention relates to the technical field of safety evaluation of fruits and vegetables, in particular to a method for evaluating the safety of fruits and vegetables with zebrafish. Background technique [0002] Vegetables and fruits are indispensable consumer goods in people's daily life. The production bases of vegetables and fruits in my country are mainly concentrated in the suburbs of cities and towns. During the cultivation process, they are easily polluted by industrial wastewater, domestic sewage, pesticides and other toxic and harmful substances. [0003] The most serious pollution problem of vegetables and fruits is pesticide residues, especially the crops grown in summer, because of high temperature and rainy, frequent pests, large amount of pesticide application and frequent frequency, so the pesticide residues are relatively high. [0004] Industrial wastewater contains many harmful substances, such as cadmium, lead, mercury, phenol and...

AI Technical Summary

Problems solved by technology

[0007] 1) Due to historical reasons, most of the detection indicators in the existing national standards are formulated with reference to foreign standards, but whether the toxicity of chemicals will show toxicity differences due to racial differences, there is currently no large sample data verification support, which means , there is uncertainty about whether a chemical that is harmless to Caucasians at standard doses is also harmless to Chinese people

[0008] 2) Unable to detect the synergistic toxicity of different chemical substances to the human body

Due to the unknown physical and chemical properties of different chemicals, there is uncertainty about whether they can produce synergistic effect or synergistic effect when interacting; it makes it more difficult to judge the synergistic toxicity of the sample to be tested than the toxicity of a single toxic substance

[0009] 3) Unknown additives cannot be detected, only known chemical substances can be detected

If there are analogues, derivatives of known chemical substances and their metabolites in the human body as unknown additives, it is difficult to detect

[0010] 4) There are many types of toxic substances, and considering cost factors such as time, manpower, and material resources, it is impossible to cover everything and achieve full inspection

[0013] After searching the literature, it is found that the current zebrafish models for safety evaluation are all drugs and cosmetics, and there is no application in the field of vegetables and fruits.

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