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Method for evaluating sleep improvement effect of dairy product by zebra fish

A technology for dairy products and zebrafish, which is applied in the field of efficacy evaluation of dairy products, can solve the problems of short modeling cycle, influence detection results, and high mortality of model animals, and achieve scientific and reasonable detection methods, high detection accuracy, and reduced detection costs. Effect

Active Publication Date: 2020-06-19
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The animal models involved in the aforementioned literature are traditional model animals such as mice and fruit flies, which have the following common technical deficiencies: short modeling period, high mortality rate of model animals, instability of animal models affecting test results, etc.
[0013] ①The above-mentioned literatures are all for the evaluation of the sleep-improving effect of specific drugs. It is well known that the ingredients contained in drugs and dairy products are different, and the differences in physical and chemical properties are also extremely significant. Therefore, the zebrafish insomnia model established in the above-mentioned literature is not applicable to For the evaluation of dairy products (especially "efficacy milk"), a more reasonable zebrafish evaluation model needs to be rebuilt
[0015] In summary, how to provide a suitable method for evaluating the sleep-improving efficacy of dairy products with zebrafish, to study whether functional dairy products can improve sleep for patients with sleep disorders caused by mental damage, is the "efficacy" on the market. It is a technical problem that has not been solved by those skilled in the art, and it is also a major technical challenge for the establishment of testing standards for functional dairy products.

Method used

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  • Method for evaluating sleep improvement effect of dairy product by zebra fish
  • Method for evaluating sleep improvement effect of dairy product by zebra fish
  • Method for evaluating sleep improvement effect of dairy product by zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Determining the processing time of dairy products

[0049] 1. Purpose of the experiment

[0050] In this experiment, only the processing time of dairy products was changed (set to 4h and 24h), and the rest of the experimental conditions were kept unchanged to explore the influence of different processing times on the detection of water quality in order to determine the optimal processing time of dairy products.

[0051] 2. Experimental reagents

[0052] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China); NO 2- (Tetra, USA).

[0053] 3. Experimental method

[0054] Randomly select 5dpf normal wild-type AB strain zebrafish in a 6-well plate, with 30 tails per well (ie, each experimental group, the same below); add the same common dairy product (ie, no functional substance added) to each well in a water-soluble manner. Ordinary milk, referred to as "ordinary milk", the same below), the concentration of ordinary milk is 2%, t...

Embodiment 2

[0061] Embodiment 2 determines the processing concentration of dairy products

[0062] 1. Purpose of the experiment

[0063] In this experiment, only the treatment concentrations of dairy products were changed (set to 2%, 3%, 4%, 5%, 6% and 7%), and the rest of the experimental conditions were unchanged to explore the effects of different treatment concentrations on the death of zebrafish. situation, the effect of fast movement distance of zebrafish to determine the optimal dairy treatment concentration.

[0064] 2. Experimental reagents

[0065] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China); 96-well plate (Fisher Scientific, China); behavioral analyzer (V3.11, ViewPoint Life Sciences).

[0066] 3. Experimental method

[0067] Randomly select 5dpf normal wild-type AB strain zebrafish in a 6-well plate, with 30 fish in each well; set up the following experimental groups: normal control group and dairy product group, and in the dairy pr...

Embodiment 3

[0079] Embodiment 3 determines the sensitivity of behavior analyzer

[0080] 1. Purpose of the experiment

[0081] In this experiment, only the sensitivity of the behavior analysis instrument (set to Max 20 and Max 32) was changed, and the rest of the experimental conditions were kept unchanged to explore the influence of different sensitivities on the rapid movement distance of zebrafish, so as to determine the best behavior analyzer sensitivity.

[0082] 2. Experimental reagents

[0083] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China); 96-well plate (Fisher Scientific, China); behavioral analyzer (V3.11, ViewPoint Life Sciences).

[0084] 3. Experimental method

[0085] Randomly select 5dpf normal wild-type AB strain zebrafish in a 6-well plate, 30 tails per well; set up the following experimental groups: normal control group, model control group, positive control group and dairy product group, the capacity of each well is 3mL, Three...

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Abstract

The invention provides a method for evaluating the sleep improvement effect of a dairy product by using zebra fish. Influence factors such as dairy product treatment time selection, dairy product treatment concentration selection and instrument parameter selection are investigated, a zebra fish model capable of evaluating the sleep improvement effect of the dairy product is successfully constructed, the detection accuracy is high, the detection method is scientific and reasonable, the modeling period is short, so that the detection cost is greatly reduced, and the practical feasibility is provided for popularizing and popularizing the detection method to broad masses of people, enterprises and governments.

Description

technical field [0001] The invention relates to the technical field of dairy product efficacy evaluation, in particular to a method for evaluating the sleep-improving efficacy of dairy products with zebrafish. Background technique [0002] Dairy products are popular for their easily absorbed, highly nutritious foods. With the development of my country's economy and the improvement of residents' consumption level, the consumption of dairy products has also increased rapidly, and the consumption demand for dairy products has gradually expanded, slowly extending from ordinary dairy products to functional dairy products. As people's demand for dairy products has been upgraded from traditional delicious and nutritious consumption to healthy and functional consumption demands, the corresponding dairy product market is also showing a trend of diversification, and various "efficacy milk" emerge in endlessly. [0003] In the current market, when various dairy products are endowed wi...

Claims

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Application Information

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IPC IPC(8): G01N33/04A01K61/10
CPCG01N33/04A01K61/10A01K2227/40A01K2207/20A01K2267/03Y02A40/81
Inventor 俞航萍李红群梁晓晴夏波沈锡明李春启
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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