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Detection method of high-quality Sihuang dysentery-stopping granules

A quality inspection method, the technology of Sihuang Zhili Granules, is applied in the field of quality detection of high-quality Sihuang Zhili Granules, and can solve problems such as checking or detecting the quality of Sihuang Zhili Granules

Active Publication Date: 2020-04-03
山东琪康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In the above techniques, Sihuang Zhili Granules are boiled and concentrated in ordinary water, and the effective substances are obviously degraded after high temperature. In addition, there is no comprehensive inspection or detection method for the quality of Sihuang Zhili Granules. Therefore, we are looking for A relatively complete and comprehensive detection method is extremely important

Method used

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  • Detection method of high-quality Sihuang dysentery-stopping granules
  • Detection method of high-quality Sihuang dysentery-stopping granules
  • Detection method of high-quality Sihuang dysentery-stopping granules

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Experimental program
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Effect test

Embodiment 1

[0067] The HPLC methodology research of baicalin in embodiment 1 Scutellaria baicalensis

[0068] 1) Instruments and reagents: Agilent 1100 high performance liquid chromatography, VWD detector. Acetonitrile was chromatographically pure, other reagents were analytically pure, and water was double distilled water.

[0069] 2) Chromatographic conditions: Kromasil C 18 Chromatographic column (4.6mm×250mm, 5μm); mobile phase acetonitrile (A)-0.1% phosphoric acid solution (B) detection wavelength is 346nm; flow rate 1mL / min. The number of theoretical plates should not be less than 2500 based on the peak of baicalin in Scutellaria baicalensis.

[0070] time (min) Mobile phase A(%) Mobile phase B(%) 0-10 15 85 10-20 28 72 20-30 28 72

[0071] 3) Preparation of the test solution: Preparation of the test solution: take an appropriate amount of Sihuang Zhili Granules, grind finely and take 2g, accurately weigh it, put it in a conical flask, and accur...

Embodiment 2

[0090] Example 2: Identification of Coptidis Rhizoma Coptidis in Sihuang Zhili Granules by Thin Layer Chromatography includes the following steps:

[0091] 1) Preparation of the test solution: Take 10g of this product, add 2.5g of diatomaceous earth, grind well, add 50mL of methanol, ultrasonically extract for 30min, shake, filter, evaporate the filtrate to dryness, add 20mL of water to dissolve the residue, extract twice with ether , 20mL each time, combine the ether solution, evaporate to dryness at low temperature, add 2mL of methanol to the residue to dissolve, and use it as the test solution;

[0092] 2) Preparation of the reference substance solution: take the berberine hydrochloride reference substance and add ethanol to make a solution containing 1 μL per 1 mL, as the reference substance solution; take 2 g of the Coptis rhizome reference drug, add 20 mL of methanol, and prepare the reference drug solution in the same way;

[0093] 3) Spotting and unfolding: Take 2-5 μL...

Embodiment 3

[0094] Embodiment 3: identification of Cortex Phellodendron in Sihuang Zhili Granules by thin layer chromatography comprises the following steps:

[0095] 1) Preparation of the test solution: take 10 g of this product, grind it finely, add 50 mL of methanol, ultrasonically extract for 30 min, filter, evaporate the filtrate to dryness, add 20 mL of water to the residue to dissolve, extract the extracted water three times with ethyl acetate, 20 mL each time, adjust the pH value of the extracted water to 10-11 with ammonia test solution, extract once with 20 mL of chloroform, evaporate the chloroform to dryness, add 2 mL of methanol to the residue to dissolve, and use it as the test solution;

[0096] 2) Preparation of reference substance solution: get cortex phellodendron hydrochloride reference substance, add methanol to make a solution containing 0.5 mg per 1 mL, as the reference substance solution;

[0097] 3) Spotting and development: Take 1-2 μL of each of the above two sol...

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Abstract

The present invention discloses a detection method of high-quality Sihuang dysentery-stopping granules. According to the quality detection method, the coptis chinensis, golden cypress, rheum officinale, scutellaria baicalensis, isatis roots and liquorice in a preparation are identified through thin-layer chromatography, and meanwhile the content of baicalin in the preparation is determined throughan HPLC. After a quality process is changed, the content of baicalin is remarkably increased, and in combination with the detection method provided by the invention, the detection result is stable and reliable, the specificity is strong, the reproducibility is good, the quality of the Sihuang dysentery-stopping granules can be comprehensively and effectively controlled, the product quality in themarket can be stabilized, and the veterinary drug safety and effectiveness can be ensured, so that the market requirements can be better met.

Description

technical field [0001] The invention belongs to the technical field of veterinary drug detection, and relates to a method for detecting the quality of high-quality Sihuang Zhili granules. The method can be used for comprehensive quality evaluation of Sihuang Zhili granules after quality improvement. Background technique [0002] Sihuang Zhili Granules are granules made of Coptis chinensis, Phellodendron Phellodendron, Rhubarb, Astragalus, Banlangen, and licorice, which are extracted and processed into Sihuang Zhili extract, and then mixed with sucrose and dextrin in a certain proportion. The efficacy of fire and dysentery is mainly used clinically for the treatment of damp-heat diarrhea and chicken colibacillosis, such as damp-heat diarrhea, pullorum, yellow diarrhea caused by E. White dysentery, watery diarrhea, etc.; diarrhea caused by Escherichia coli and Salmonella, yellow and white diarrhea, infectious gastroenteritis, epidemic diarrhea, rotavirus diarrhea and other vir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/91G01N30/94G01N30/06
CPCG01N30/06G01N30/91G01N30/94
Inventor 王友华
Owner 山东琪康生物技术有限公司
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