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Method for detecting performance of air purifying product on killing bacteriophage in air

A technology for air purification and air purification, applied in the direction of bacteriophage, virus/bacteriophage, and microorganism-based methods, to achieve the effect of ensuring recovery concentration, high purity, and good stability

Inactive Publication Date: 2018-10-19
广州市微生物研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention can solve the problem of evaluating the virus purification effect of existing air purification products, and provides the purification and production of the virus stock solution and the determination of the titer. activity and recovery concentration; moreover, the use of the test chamber and the blank chamber ensures the biological safety of the test and the airtightness of the environment; in addition, this method introduces the natural attenuation rate of the blank when calculating the purification efficiency, which fully guarantees the objectiveness of the detection conclusion sex and authenticity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Determination of the ability of air purification products to purify phages in the air (for convenience of description, take the test of Escherichia coli phage Phi-X174 as an example)

[0061] This embodiment provides a method for measuring the effect of air purification products on phage purification, comprising the following steps:

[0062] (1) Prepare fresh phage stock solution: take 10 mL of Escherichia coli suspension multiplied the day before and 5 mL of refrigerated phage stock solution in a ratio of 2:1, and place it statically at 36±1°C for 15-20 min. Obtain a mixed solution; then take 10 mL of the above mixed solution and mix it with 90 mL of NB liquid medium, and incubate at 37°C for 18-20 h to obtain a mixed culture solution; centrifuge at 3000 r / min for 10 min, and then remove the supernatant Repeat the centrifugation once; the supernatant after the centrifugation is first coarsely filtered once with 0.45 μm filter paper, and then finely filtered o...

Embodiment 2

[0069] Example 2 Determination of the ability of air purification products to purify phages in the air (initial sample sampling volume)

[0070] The assay method of a kind of air purification product that present embodiment provides to phage purification effect is basically the same as embodiment 1, and difference is: the initial sample sampling volume of the phage in test chamber and blank chamber is 15 L; The sampling volumes of the residual samples of phage in the blank compartment were 84.9 L and 15 L, respectively.

[0071] The initial concentration and residual concentration of phage in the test chamber of this embodiment are respectively 1.12×10 5 PFU / m 3 and 3.84×10 3 PFU / m 3 , the initial and residual concentrations of phage in the blank compartment were 1.09×10 5 PFU / m 3 and 7.86×10 4 PFU / m 3 . According to the change value of phage concentration in the blank chamber, the natural attenuation rate can be calculated as 27.89%; then according to the change v...

Embodiment 3

[0072] Example 3 Determination of the ability of air purification products to purify phages in the air (initial sample sampling volume)

[0073] The assay method of a kind of air purification product that present embodiment provides to phage purification effect is basically the same as embodiment 1, and difference is: the initial sample sampling volume of the phage in test chamber and blank chamber is 5 L; The sampling volumes of the residual samples of phage in the blank compartment were 84.9 L and 5 L, respectively.

[0074] The initial concentration and residual concentration of phage in the test chamber of this embodiment were 9.86×10 4 PFU / m 3 and 3.27×10 3 PFU / m 3 , the initial concentration and residual concentration of phage in the blank compartment were 9.78×10 4 PFU / m 3 and 7.06×10 4 PFU / m 3 . According to the change value of the phage concentration in the blank chamber, the natural attenuation rate can be calculated as 27.81%; then according to the chang...

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Abstract

The invention discloses a method for detecting the performance of an air purifying product on killing bacteriophage in the air. The method comprises following steps: step one, preparing a fresh bacteriophage stock solution, measuring the titer of the stock solution, and diluting the stock solution; step two, setting same conditions of a test cabin and a blank cabin, and placing an purifying product in the test cabin; step three, releasing the diluted bacteriophage solution in an aerosol mode, stirring 2 to 5 minutes, and then allowing the system to stand still for 2 to 5 minutes; step four, collecting the primary samples in the test cabin and blank cabin, then starting the purifying product in the test cabin, and after a while, collecting the residue samples in the test cabin and the blankcabin; and step five, culturing, calculating the natural attenuation rate, and evaluating the purifying effect. The provided method can detect multiple bacteriophage host bacteria, and has a wide application range. Furthermore, natural attenuation rate is taken into account, the objectiveness and realness of the detection results are ensured, and the method can be used to accurately evaluate thevirus killing effect of an air purifying product.

Description

technical field [0001] The invention belongs to the technical field of microbial analysis and detection. More specifically, it relates to a method for detecting the ability of air purification products to purify phages in the air. Background technique [0002] At present, the global environment is deteriorating. According to the statistics of the World Health Organization, nearly 17 million people die every year due to infection of various diseases, the most serious of which is respiratory tract infection. Living in turbid air for a long time will cause harm to the nervous system, respiratory system, and immune system of the human body, leading to decreased immunity and related diseases. At the same time, with the continuous improvement of the quality of life, people pay more attention to the health of their living environment. So many health care products have come out one after another, including air purification products. Air purification products are a kind of equipme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/04C12R1/92
CPCC12N7/00C12Q1/04C12N2795/00051
Inventor 黄东浪赵培静丁年平杜少平明飞平夏枫耿万分龙陈勉华
Owner 广州市微生物研究所
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