Dogwood fruit extract and its preparation process

A preparation process and extract technology, applied in the field of traditional Chinese medicine, can solve problems such as low triterpene acid content, achieve the effects of small effective dose, lower blood sugar level, and safe and economical extraction process

Active Publication Date: 2006-02-15
SUZHOU SIXTH PHARMA PLANT OF JIANGSU WUZHONG PHARMA GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of triterpene acids in Cornus officinalis, there is no process that is really effective in producing high levels of triterpene acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Heat 1 kg of Cornus officinalis with 10 times the amount of 95% ethanol to reflux for 1.5 h, and extract twice. Combine the extracts and concentrate until there is no alcohol smell. The concentrated solution was left to stand at low temperature for more than 24 hours, the solid and the clear liquid were separated by filtration, the solid was washed with hot water until neutral, and the washing liquid was combined with the clear liquid. Heat and dissolve the solid with 95% ethanol, filter, add 20% sodium hydroxide alkali solution to the filtrate, filter to remove fat-soluble impurities, add 10% dilute hydrochloric acid solution to the filtrate to adjust the pH to 1-2, and precipitate white to yellow solids, filter, and use for solids Recrystallize with 95% ethanol to obtain extract A.

[0053] The lower supernatant was applied to a HPD-100 macroporous resin column, washed with water and 5% ethanol to remove impurities, and eluted with 50% ethanol. The eluate was collect...

Embodiment 2

[0056] Heat 1 kg of Cornus officinalis with 10 times the amount of 90% ethanol to reflux for 2 hours, and extract twice. Combine the extracts and concentrate until there is no alcohol smell. The concentrated solution was left to stand at low temperature for more than 24 hours, the solid and the clear liquid were separated by filtration, the solid was washed with hot water until neutral, and the washing liquid was combined with the clear liquid. Heat the solid with absolute ethanol to dissolve, filter, add 35% sodium hydroxide alkali solution to the filtrate, filter to remove fat-soluble impurities, add 8% dilute hydrochloric acid solution to the filtrate to adjust the pH to 1-2, and precipitate white to yellow solids, filter, and use for solids Extract A was obtained by recrystallization from absolute ethanol.

[0057] The subnatant was applied to a D101 macroporous resin column, washed with water and 5% ethanol to remove impurities, and eluted with 40% ethanol. The eluate wa...

Embodiment 3

[0060] Heat 1 kg of Cornus officinalis with 10 times the amount of 80% ethanol to reflux for 1 hour, and extract 3 times. Combine the extracts and concentrate until there is no alcohol smell. The concentrated solution was left to stand at low temperature for more than 24 hours, the solid and the clear liquid were separated by filtration, the solid was washed with hot water until neutral, and the washing liquid was combined with the clear liquid. Heat and dissolve the solid with 90% ethanol, filter, add 15% sodium hydroxide alkali solution to the filtrate, filter to remove fat-soluble impurities, add 5% dilute hydrochloric acid solution to the filtrate to adjust the pH to 1-2, and precipitate white to yellow solids, filter, and use for solids Recrystallize with 95% ethanol to obtain extract A.

[0061] The subnatant was applied to an AB-8 macroporous resin column, washed with water and 5% ethanol to remove impurities, and eluted with 55% ethanol, and the eluate was collected a...

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PUM

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Abstract

The invention discloses a dogwood fruit extract and the process for preparation, wherein the extract is prepared from oleanolic acid, ursolic acid, loganin and morroniside by the ratio of (1-5) : (6-12) : (12-20) : 25. The preparing process comprises the following steps: extracting dogwood fruit with alcohol, concentrating the extract and stewing at low temperature, subjecting the upper layer solid to acid and alkaline treatment, obtaining extract A, separating the lower layer clear liquid with macroscopic resin columns, obtaining extract B, finally mixing the extract A and the extract B. The prepared dogwood fruit extract can be used for treating diabetes and the complications.

Description

technical field [0001] The invention relates to a cornus officinalis extract, a preparation method and application of the extract, and belongs to the field of traditional Chinese medicine. Background technique [0002] Diabetes is a group of endocrine-metabolic diseases characterized by hyperglycemia. It has become a frequently-occurring disease in recent years. It has become or is becoming the third most serious chronic disease after tumors and vascular diseases in both developed and developing countries. Non-communicable diseases are listed by WHO as one of the world's three chronic diseases. At present, there is still no cure drug, and the clinical treatment is mostly western medicine, but there are side effects such as hypoglycemia, gastrointestinal reactions, and liver toxicity. With the prolongation of treatment time, the hypoglycemic effect tended to decrease. Therefore, traditional Chinese medicine has become an important way to find new hypoglycemic drugs due to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/40A61P3/10
Inventor 印敏楼凤昌曹庆先
Owner SUZHOU SIXTH PHARMA PLANT OF JIANGSU WUZHONG PHARMA GROUP
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