Method for extraction and purification of four iridoid glycosides in cornus officinalis

A technology for iridoid glycosides and cornus cornus, which is applied in the field of traditional Chinese medicine extraction, can solve the problems of not extracting and purifying iridoid glycosides and the like, and achieves the effects of convenient industrial production, high purity and low cost

Inactive Publication Date: 2013-10-23
天津科丝美特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the extraction and purification of the above four iri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting and purifying four iridoid glycosides from Cornus officinalis, comprising the steps of:

[0021] (1) Precisely weigh Cornus officinalis, heat and reflux for 3 hours with 10 times the mass of ethanol aqueous solution with a concentration of 50% by volume, filter, extract 3 times, and combine the filtrates;

[0022] (2) Purify the filtrate with a mixed macroporous resin composed of HPD300, LSA30 and SP825, and elute with 3BV of 50% ethanol aqueous solution at a flow rate of 1.5BV / h, collect and wash Deliquified and concentrated to obtain a mixture containing four iridoid glycosides, ie, loganin, swertigin, morroniside and cornelin. The mass ratio of HPD300, LSA30 and SP825 in the mixed macroporous resin is 2:3:1.

[0023] The transfer rate of loganin in the mixture was 78.2%; the transfer rate of swertigin was 75.8%;

Embodiment 2

[0025] A method for extracting and purifying four iridoid glycosides from Cornus officinalis, comprising the steps of:

[0026] (1) Accurately weigh Cornus officinalis, heat and reflux for 1 hour with 5 times the mass of ethanol water solution with a concentration of 90% by volume, filter, extract 4 times, and combine the filtrates;

[0027] (2) Purify the filtrate with a mixed macroporous resin composed of HPD300, LSA30 and SP825, and elute with 2BV of 60% ethanol aqueous solution at a flow rate of 2BV / h, collect and elute The solution was concentrated to obtain a mixture containing four iridoid glycosides, namely loganin, swertigin, morroniside and cornel schizoside. The mass ratio of HPD300, LSA30 and SP825 in the mixed macroporous resin is 1:2:3.

[0028] The transfer rate of loganin in the mixture was 78.0%, that of swertigin was 74.3%, that of morroniside was 76.9%, and that of schizoside was 74.1%.

Embodiment 3

[0030] A method for extracting and purifying four iridoid glycosides from Cornus officinalis, comprising the steps of:

[0031] (1) Accurately weigh Cornus officinalis, heat and reflux for 2 hours with 8 mass times of 70% ethanol aqueous solution by volume, filter, extract 3 times, and combine the filtrates;

[0032] (2) Purify the filtrate with a mixed macroporous resin composed of HPD300, LSA30 and SP825, and elute with 1BV of 80% ethanol aqueous solution at a flow rate of 1BV / h, collect and elute The solution was concentrated to obtain a mixture containing four iridoid glycosides, namely loganin, swertigin, morroniside and cornel schizoside. The mass ratio of HPD300, LSA30 and SP825 in the mixed macroporous resin is 2:4:1.

[0033] The transfer rate of loganin in the mixture was 77.7%, that of swertigin was 73.8%, that of morroniside was 76.9%, and that of schizoside was 75.4%.

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Abstract

The invention discloses a method for extraction and purification of four iridoid glycosides in cornus officinalis. The method comprises the following steps of 1, accurately weighing cornus officinalis, carrying out heating reflux extraction by an ethanol aqueous solution having a volume concentration of 40-90% for 1-4h, wherein the weight of the ethanol aqueous solution is 5-15 times the weight of the weighed cornus officinalis, carrying out filtration, carrying out extraction 2-4 times, and merging the filtrate, and 2, carrying out purification of the merged filtrate by a mixed macroporous resin comprising HPD300, LSA30 and SP825, carrying out elution by 1-4BV of an ethanol aqueous solution having a volume concentration of 20-80% at an elution flow rate of 1-3BV/h, collecting the eluent, and carrying out condensation to obtain a mixture of four iridoid glycosides. The method has the advantages that 1, processes are simple and safe; a process is short; a cost is low; and industrial production is convenient; and 2, through extraction and purification by the mixed resins, high-purity loganin, swertiamarin, morroniside and cornuside are obtained.

Description

technical field [0001] The invention belongs to the field of extracting traditional Chinese medicines, and in particular relates to a method for extracting four iridoid glycosides, namely loganin, swertigin, morroniside and fenugreek schizoside, from cornus officinalis. technical background [0002] Cornus officinalis is the dried and mature pulp of Cornus officinalis. Cornus officinalis has the effects of nourishing the liver and kidney, astringent essence and detoxification, etc. It is used to treat dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, enuresis, frequent urination, metrorrhagia, profuse sweating, prostration, internal heat and quenching thirst. It is listed in various editions of "Chinese Pharmacopoeia" Both are loaded. The earliest records about the medicinal use of this product can be found in "Shen Nong's Materia Medica", which is listed as a middle grade. Its main chemical components are organic acids, polysaccharides, iridoid glyc...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 王艳
Owner 天津科丝美特生物科技有限公司
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