Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multi-index fast detection method of dogwood medicinal materials

A detection method, the technology of Cornus officinalis, applied in the field of near-infrared detection, can solve the problems of time-consuming detection method, difficult production practice, laborious and other problems, and achieve the effects of stable product quality, saving production cost, improving production efficiency and economic benefits

Inactive Publication Date: 2015-04-29
ZHEJIANG UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional detection method is time-consuming and laborious, and it is difficult to be widely used in production practice. Therefore, it is necessary and promising to develop a method that can quickly detect the quality of Cornus officinalis for on-site screening of medicinal materials and comprehensive quality control.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-index fast detection method of dogwood medicinal materials
  • Multi-index fast detection method of dogwood medicinal materials
  • Multi-index fast detection method of dogwood medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Data collection of near-infrared spectroscopy of Cornus officinalis

[0037] Cornus officinalis medicinal materials from different origins were crushed and passed through a 60-mesh sieve to obtain the medicinal powder of Cornus officinalis with a relatively uniform particle size; 2 g of the medicinal materials of Cornus officinalis was precisely weighed and placed in a weighing bottle to keep the surface of the powder flat, and collected by diffuse reflection method Near-infrared spectrum, using air as a reference, the number of scans is 32, and the resolution is 8 cm -1 , the scanning spectral range is 4000-10000 cm -1 , each batch of sample scans was repeated 5 times, and the average spectrum was taken. See the appendix for the near-infrared original absorption spectrum of the medicinal powder of Cornus officinalis figure 1 .

[0038] (2) Determination of moisture content in Cornus officinalis

[0039] Moisture determination method: The moisture content of Co...

Embodiment 2

[0046] (1) Near-infrared spectrum collection: the near-infrared spectrum used is the spectrum collected by the moisture content modeling in Example 1

[0047] (2) Determination of the extract content of Cornus officinalis

[0048] Determination method of extract content: Take about 2g of Cornus officinalis medicinal material, put it in a 250mL Erlenmeyer flask, add 50mL of water, seal it tightly, soak in cold and weigh it. Shake it from time to time for the first 6 hours, then let it stand for 18 hours, weigh the mass, subtract the lost mass with insufficient water, and shake well. Centrifuge in a 15 mL centrifuge tube for 30 min at a speed of 3800 r / min, accurately measure 10 mL of the supernatant, and place it in a flat bottle that has been dried to constant weight (X 0 ), evaporated to dryness on a water bath, dried at 105 °C for 3 h, cooled in a desiccator for 30 min, and quickly and accurately weighed (X 2 ). Calculate the extract content (%) of the test sample based o...

Embodiment 3

[0055] (1) Near-infrared spectrum collection: the near-infrared spectrum used is the spectrum collected by the moisture content modeling in Example 1

[0056] (2) Determination of the content of morroniside and loganin in Cornus officinalis

[0057] The content of morroniside and loganin was determined by high performance liquid chromatography: a. The pretreatment method was: take about 0.1 g of Cornus officinalis medicinal material powder (passed through a 60-mesh sieve), accurately weigh it, put it in a stoppered conical flask, and accurately add Heat 25 mL of 80% methanol to reflux for 1 h, weigh again, and use 80% methanol to make up for the weight loss. Centrifuge the extract in a 1.5mL centrifuge tube for 10 min at 13000 r min -1 , take the supernatant, vested. b. Liquid chromatography conditions: chromatographic column: Agilent Eclipse XDB-C18 analytical column (4.6×250mm, 5μm); mobile phase: acetonitrile-water (15:85, v / v); detection wavelength 240 nm, flow rate 0.8...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a multi-index fast detection method of dogwood medicinal materials. The multi-index fast detection method comprises the following steps of: acquiring the dogwood medicinal materials; determining the contents of morroniside and loganin in the dogwood by using a high-performance liquid chromatography, determining the content of water by using a drying method, and determining the content of extract by using a cold-extraction method; collecting near-infrared spectrum data of dogwood medicinal material powder; preprocessing spectrum at the modeling waveband of the near-infrared spectrum; establishing a fast detection model for the water, the extract and the morroniside and the loganin in the dogwood medicinal materials; and using the established model for fast determination of the water, the extract and the morroniside and the loganin in the dogwood medicinal materials. The multi-index fast detection method provided by the invention has the advantages that a near-infrared spectrum technology is introduced as the fast detection method of the contents of the water, the extract, the morroniside and the loganin in the dogwood medicinal materials; and compared with the traditional method, the established analysis method has the advantages that whether the quality of the medicinal materials is qualified can be fast judged, whether the medicinal materials can enter the following production-process links can be determined, the requirements of fastness and high efficiency in production can be met, and the application prospect for site medicinal-material screening and comprehensive quality evaluation is achieved.

Description

technical field [0001] The invention belongs to the field of near-infrared detection, and in particular relates to a multi-index rapid detection method of cornus officinalis. Background technique [0002] Cornus officinalis Cornus officinalis Dried ripe pulp of Sieb. et Zucc. In the clinical practice of traditional Chinese medicine, its mature fruit is used as medicine after the pitting is removed. one. The 2010 edition of the Chinese Pharmacopoeia records that Cornus officinalis has the functions of nourishing the liver and kidney, astringent and solidifying, and is used for dizziness, tinnitus, waist and knee pain, impotence and nocturnal emission, enuresis, frequent urination, metrorrhagia, profuse sweating, prostration, internal heat and quenching thirst. The chemical composition of Cornus officinalis is very complex, mainly including organic acids and their esters and iridoid glycosides as the main active ingredients. In recent years, scholars at home and abroad ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563G01N21/359
Inventor 刘雪松孙芬芳金叶吴永江陈勇
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com