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Method for quickly detecting six ingredients of dogwood by use of UFLC

A rapid determination of dogwood technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of poor separation effect and long time consumption, improve the separation degree and detection sensitivity, reduce the consumption of solvent, and the method is simple Effect

Inactive Publication Date: 2015-10-21
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, HPLC (high performance liquid chromatography) is mostly used for the determination of the above-mentioned effective components of Cornus officinalis, which takes a long time and has poor separation effect

Method used

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  • Method for quickly detecting six ingredients of dogwood by use of UFLC
  • Method for quickly detecting six ingredients of dogwood by use of UFLC
  • Method for quickly detecting six ingredients of dogwood by use of UFLC

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preparation example Construction

[0028] 1) Preparation of the test solution: Precisely weigh raw Cornus officinalis or wine-steamed product powder, extract it with methanol solution with a volume fraction of 60% to 100%, mix it with 20 mL of methanol solution per gram of Cornus officinalis powder, and weigh it. Heavy, extract under ultrasonic and microwave conditions for 3 to 8 minutes, then suction filter, and the obtained filtrate is replenished to the original weight with methanol solution, shaken well, and passed through a 0.22 μm microporous membrane to obtain the test solution. The conditions are: microwave power 600W, ultrasonic power 360W, temperature 50°C;

[0029] 2) Preparation of the reference substance solution: Accurately weigh the reference substances of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, swertiroside and cornel glycoside respectively, and adopt a volume fraction of 60% to 100%. After the methanol solution was dissolved, it was diluted with methanol solution to obtain 6...

Embodiment 1

[0038] 1) Preparation of the test solution: Precisely weigh 1.00g of Cornus officinalis powder, place it in a XO-200 special glass reaction bottle, add 20mL of 80% methanol solution to seal, weigh, connect the reflux device, and ultrasonically Extract in a microwave combined reaction system for 5 minutes, wherein the microwave power is 600W, the ultrasonic power is 360W, and the temperature is 50°C, then take it out, filter it with suction, and make up the filtrate to the original weight with 80% methanol solution, shake it well, and pass through a 0.22μm microporous filter The membrane was used as the test solution.

[0039] 2) Preparation of the reference substance solution: Accurately weigh 1.00 mg of the reference substance of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, swertiroside, and cornel glycoside, respectively, and place them in a 1.0 mL volumetric flask. Add chromatographic methanol with a volume fraction of 80% to dissolve and constant volume, and d...

Embodiment 2

[0054] 1) Preparation of the test solution: Precisely weigh raw Cornus officinalis or wine-steamed product powder, extract with methanol solution with a volume fraction of 60%, mix according to the ratio of adding 20 mL of methanol solution per gram of Cornus officinalis powder, and weigh it. Extract under ultrasonic and microwave conditions for 3 minutes, then suction filter, the obtained filtrate is replenished to the original weight with methanol solution, shake well, and pass through a 0.22 μm microporous membrane to obtain the test solution, wherein the specific conditions of ultrasonic microwave are: microwave power 600W, ultrasonic power 360W, temperature 50°C;

[0055] 2) Preparation of the reference substance solution: Accurately weigh the reference substances of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, swertigin and cornel glycoside respectively, and use a methanol solution with a volume fraction of 60% After dissolving, dilute with methanol soluti...

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Abstract

The invention discloses a method for quickly detecting six ingredients of dogwood by use of UFLC (Ultra Fast Liquid Chromatography). The method comprises the following steps: at first, preparing a test solution; then preparing six reference solutions of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, sweroside and cornuside; adopting the UFLC for detection, and performing gradient elution: 0-3 min, 2-5% A; 3-5 min, 5-17% A; 5-10 min 17-17.5%; 10-11 min, 17.5-30% A; 11-15 min, 30-60% A; finally, calculating the result according to setting of a standard curve. The method has the advantages of being simple, quick and high in sensitivity, precision and accuracy.

Description

technical field [0001] The invention belongs to the field of detection of chemical components of traditional Chinese medicinal materials, in particular to a method for simultaneously detecting gallic acid, 5-hydroxymethylfurfural, morroniside and loganin in raw Cornus officinalis and wine-steamed products by UFLC (Ultra High Performance Liquid Chromatography) , swertoside, and cornel new glycosides. Background technique [0002] Cornus officinalis is the dry and mature pulp of Cornus offcinalis Sieb.et Zucc, a plant of the family Cornaceae, which has the effects of nourishing the liver and kidney, and astringent essence. Clinically, it is mostly used for cornus and wine cornus. Cornus cornus is good at suppressing sweat and solidifying, and is often used for night sweats due to yin deficiency; wine cornus has enhanced liver and kidney effects, and is often used for dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, and enuresis Frequent urination and o...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 康杰芳王韶君白嫄王艳珍蒋罗曼陈康健王喆之
Owner SHAANXI NORMAL UNIV
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