Method for quickly detecting six ingredients of dogwood by use of UFLC

Abstract

The invention discloses a method for quickly detecting six ingredients of dogwood by use of UFLC (Ultra Fast Liquid Chromatography). The method comprises the following steps: at first, preparing a test solution; then preparing six reference solutions of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, sweroside and cornuside; adopting the UFLC for detection, and performing gradient elution: 0-3 min, 2-5% A; 3-5 min, 5-17% A; 5-10 min 17-17.5%; 10-11 min, 17.5-30% A; 11-15 min, 30-60% A; finally, calculating the result according to setting of a standard curve. The method has the advantages of being simple, quick and high in sensitivity, precision and accuracy.

Description

technical field

[0001] The invention belongs to the field of detection of chemical components of traditional Chinese medicinal materials, in particular to a method for simultaneously detecting gallic acid, 5-hydroxymethylfurfural, morroniside and loganin in raw Cornus officinalis and wine-steamed products by UFLC (Ultra High Performance Liquid Chromatography) , swertoside, and cornel new glycosides. Background technique

[0002] Cornus officinalis is the dry and mature pulp of Cornus offcinalis Sieb.et Zucc, a plant of the family Cornaceae, which has the effects of nourishing the liver and kidney, and astringent essence. Clinically, it is mostly used for cornus and wine cornus. Cornus cornus is good at suppressing sweat and solidifying, and is often used for night sweats due to yin deficiency; wine cornus has enhanced liver and kidney effects, and is often used for dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, and enuresis Frequent urination and o...

Images