Method for extracting high-purity loganin from cornus officinalis fruits
A technology of cornus officinalis and loganin, which is applied in the field of purifying loganin, can solve the problems of being difficult to meet industrialized large-scale production, being unsuitable for industrial-scale production, and using a large amount of solvents, and achieves fewer types of solvents, easy operation, and improved performance. Effects of yield and purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] Take 1kg of dried fruit of Cornus officinalis, add 7L of ethanol with a volume fraction of 50%, heat and reflux to extract twice, each extraction for 1 hour, combine the two extracts, concentrate, add ethanol to adjust the alcohol concentration to 30%, add 1 / 20 volume 2mol / L acetic acid, react at 80°C for 2 hours, after the reaction is completed, add NaOH solution to neutralize to neutral, and concentrate the neutralized solution to dryness to obtain a dry product, which is extracted twice with 400mL ethanol under reflux, each time for 2 hours, The liquids were combined and concentrated to obtain crude loganin. The crude product was dissolved with 100 mL of methanol under reflux, and the insoluble matter was filtered while it was hot. The filtrate was cooled to room temperature, stirred and crystallized, filtered and dried, and recrystallized twice with methanol to obtain 3.8 g of loganin with a content of 96.3%.
Embodiment 2
[0028] Take 1kg of dried fruit of Cornus officinalis, add 10L of ethanol with a volume fraction of 20%, heat and reflux to extract twice, each extraction is 2 hours, combine the two extracts, concentrate, add ethanol to adjust the alcohol concentration to 5%, add 1 / 10 volume 1mol / L hydrochloric acid, react at 70°C for 3 hours, after the reaction is over, add NaHCO 3 The solution was neutralized to neutral, and the neutralized solution was concentrated to dryness to obtain a dried product, which was extracted twice with 600 mL of acetone under reflux for 2 hours each time, and the extracts were combined and concentrated to obtain a crude loganin. The crude product was dissolved in 200 mL of absolute ethanol by heating under reflux, and the insoluble matter was filtered while it was hot. The filtrate was cooled to room temperature, stirred and crystallized, filtered and dried, and recrystallized twice with absolute ethanol to obtain 3.2 g of loganin with a content of 98.1%. . ...
Embodiment 3
[0030] Take 4kg of dried fruit of Cornus officinalis, add 32L of ethanol with a volume fraction of 60%, heat and reflux to extract twice, each extraction is 2 hours, combine the two extracts, concentrate, add ethanol to adjust the alcohol concentration to 70%, add 1 / 100 volume 3mol / L hydrochloric acid, react at 60°C for 4 hours, after the reaction is completed, add NaHCO 3 The solution was neutralized to neutral, and the neutralized solution was concentrated to dryness to obtain a dry product, which was extracted twice with 1 L of methanol under reflux, each time for 2 hours, and the extracts were combined and concentrated to obtain a crude loganin. The crude product was dissolved with 800 mL of ethanol under reflux, and the insoluble matter was filtered while it was hot. The filtrate was cooled to room temperature, stirred and crystallized, filtered and dried, and recrystallized twice with ethanol to obtain 16.2 g of loganin with a content of 97.2%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com