Method for extracting and separating loganin from flowers and flower buds or branches and leaves of Loliseria hypoglauca Miq

A technology of red glandular honeysuckle and loganin, which is applied in the field of natural medicinal chemistry and can solve the problems of rare pharmacological effects

Inactive Publication Date: 2014-01-22
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Abstract
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Problems solved by technology

There are few studies on the pharmacological effects of iridoid glycosides effective parts in Lonicera. It is necessary to conduct in-depth rese

Method used

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  • Method for extracting and separating loganin from flowers and flower buds or branches and leaves of Loliseria hypoglauca Miq
  • Method for extracting and separating loganin from flowers and flower buds or branches and leaves of Loliseria hypoglauca Miq
  • Method for extracting and separating loganin from flowers and flower buds or branches and leaves of Loliseria hypoglauca Miq

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specific Embodiment 2

[0015] Extraction and isolation of loganin from honeysuckle flower and flower buds

[0016] The dried and crushed flowers and flower buds (5.0kg) of Lonicera red glandularis were soaked in 5 times the amount of 75% ethanol for 24 hours, then ultrasonically extracted for 30 minutes. volume, ultrasonic extraction for 30min., after the extracts were filtered and combined, the alcohol solvent was recovered by vacuum distillation to a large amount of foaming (relative density 1.05 / 60°C), and 0.5 to 1 times the volume of water was added to mix evenly. Ethyl acetate was extracted three times, and the solvent was recovered to obtain the ethyl acetate part; then the mother liquor was extracted three times with n-butanol in equal volume, and the n-butanol extracted part was concentrated under reduced pressure to remove the organic solvent to obtain a liquid extract. After dissolving the liquid extract with an appropriate amount of ethanol, it was subjected to polyamide column chromatogr...

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Abstract

The invention discloses a method for extracting and separating a compound loganin from flowers and flower buds or branches and leaves of a featured herbal medicine Loliseria hypoglauca Miq in Guangxi province. The method is characterized by comprising the steps of extracting through high-concentration low-grade alcohols (such as methanol and ethanol), concentrating and then extracting through ethyl acetate to remove liposoluble components, extracting with n-butyl alcohol, concentrating through polyamide column chromatography, performing SephadexLH-20 gel chromatography, eluting with low-grade alcohols (such as methanol and ethanol) as an eluent, recovering the solvent for concentrating, separating, crystallizing, and re-crystallizing to obtain loganin with the purity of more than 95%. According to the method, the natural medicine active ingredient having wide market prospects can be recovered from flowers and clipped waste branches and leaves after deflowering of Loliseria hypoglauca Miq, and the method has a tremendous promotion effect on the comprehensive utilization of Loliseria hypoglauca Miq.

Description

technical field [0001] The present invention relates to the field of natural medicinal chemistry. In particular, it relates to a method for quickly extracting and preparing loganin from honeysuckle and flower buds, or crop waste "Lonicera japonica branches and leaves" at a lower cost. Background technique [0002] The flowers and buds of Lonicera hypoglauca Miq are used as Honeysuckle Flower (Japanese Honeysuckle) in traditional Chinese medicine. The "Pharmacopia" after 2005 clearly stipulates that the flower of Lonicera hypoglauca Miq is mountain silver flower, The honeysuckle (Lonicera japonica Thunb) flowers are honeysuckle, in terms of chemical composition, they have certain differences, but not much. In Guangxi, especially in karst lava landform areas, honeysuckle is most suitable for planting, which can not only increase farmers' income, but also control environmental rocky desertification, and play a role in local water and soil conservation and natural environment r...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 宋志军缪剑华姚彩云蓝鸣生陈路杜芳黎李汉浠陈广钜吴庆华黄丹娜
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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