Detection method of pterocephalus hookeri

A detection method, the technology of pterygium, applied in the field of medicine, can solve the problems of difficult operation, complicated detection method, and inability to comprehensively evaluate the active ingredients, and achieve the effect of comprehensive detection and simple operation

Active Publication Date: 2014-12-24
SHANDONG JINHE DRUG RES DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned documents are all thin-layer or content detection methods for oleanolic acid and ursolic acid, and no detection method that can simultaneously detect loganin, ursolic acid and / or oleanolic acid has been found, resulting in the detection of Pterocephalin The result deviates greatly from the actual demand, and it is impossible to make a more comprehensive evaluation of its medicinal ingredients, and the detection method is complicated and difficult to operate

Method used

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  • Detection method of pterocephalus hookeri
  • Detection method of pterocephalus hookeri
  • Detection method of pterocephalus hookeri

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental example 1: Identification experiment of Pterocephala

[0032] Take 0.5g of pterygium powder, put it in a stoppered Erlenmeyer flask, add 50ml of methanol, seal it tightly, ultrasonically treat it for 1h, let it cool, filter, and concentrate the filtrate to 5ml as the test solution; take another loganin , An appropriate amount of oleanolic acid reference substance, add methanol to make a mixed solution containing 0.1mg of loganin and 0.3mg of oleanolic acid per 1ml, as the reference substance solution. According to the thin-layer chromatography test, draw 5 μl of each of the above two solutions, and place them on the same silica gel G thin-layer chromatography plate respectively, using cyclohexane-chloroform-methanol with a volume-to-part ratio of 5:2:2 as the developing agent, Unfold, take out, dry in the air, spray with ethanol solution of 1% vanillin sulfuric acid by volume, heat at 105°C until the spots are clearly colored, and inspect under sunlight. I...

experiment example 2

[0034] Experimental Example 2: Detection experiment of Pteracephala content

[0035] 1. Instruments, reagents and test samples

[0036] Instruments: High performance liquid chromatography: Hitachi L-2100 pump, L-2400 ultraviolet detector; Shimadzu AUW220D electronic balance.

[0037] Reference substance: loganin reference substance (National Institute for Food and Drug Control, batch number: 111640-201005); oleanolic acid reference substance (National Institute for the Control of Pharmaceutical and Biological Products, batch number: 110709-200505); ursolic acid reference substance ( China National Institutes for Food and Drug Control, batch number: 110742-201220).

[0038] 2. Selection of detection wavelength

[0039] Take loganin reference substance solution, oleanolic acid reference substance solution, ursolic acid reference substance solution and mixed control solution respectively, scan in the wavelength range of 190-600nm, and select 210-230, especially 220nm is the de...

Embodiment 1

[0113] Embodiment 1, a kind of detection method of pterygium, comprises following identification and content detection method:

[0114] Identification:

[0115]Take 0.5g of pterygium powder, put it in a stoppered Erlenmeyer flask, add 50ml of methanol, seal it tightly, ultrasonically treat it for 1h, let it cool, filter, and concentrate the filtrate to 5ml as the test solution; take another loganin , oleanolic acid reference substance, add methanol to make a mixed solution containing 0.1mg of loganin and 0.3mg of oleanolic acid per 1ml, as the reference substance solution; test according to thin-layer chromatography, absorb 5 μl of each of the above two solutions , respectively point on the same silica gel G thin-layer chromatography plate, use cyclohexane-chloroform-methanol with a volume-to-volume ratio of 5:2:2 as the developer, develop, take out, dry in the air, and spray with a volume percentage of 1%. Vanillin sulfuric acid ethanol solution, heated at 105°C until the sp...

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Abstract

The invention relates to a detection method of pterocephalus hookeri. The detection method is capable of simultaneously detecting loganin, ursolic acid and / or oleanolic acid in pterocephalus hookeri. The separation and content measurement of loganin, ursolic acid and / or oleanolic acid in great polarity difference can be realized by virtue of the simple gradient variation of the flow phase, and the real detection requirement can be further met.

Description

technical field [0001] The invention relates to a detection method of Pterocephala, which belongs to the technical field of medicine. Background technique [0002] Pterocephalus hookeri (Clarke) Hoeck is Pterocephalus hookeri (Clarke) Hoeck, a plant of the genus Pterocephalus in the family Disperaceae. Modern research shows that: Pterocephalus mainly contains saponins, iridoids and other chemical components, and it is believed that oleanolic acid and ursolic acid are the main active ingredients in Pterocephalus. Studies in recent years have shown that they have significant anti-inflammatory properties. The effect of loganin is also one of the main active ingredients of Pterocephalus. [0003] In the prior art, there are many studies on the detection method of Pterostella chinensis. The detection method of Chinese patent CN103792303A Pterocephala medicinal material provides the detection method of Pterocephalic medicinal material, which is determined by ultra-fast liquid chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 班玛才仁孙绪丁任松鹏赵丽丽
Owner SHANDONG JINHE DRUG RES DEV
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