The invention discloses a
high performance liquid chromatography detection method of free methoxy group noradrenaline and methoxy group in
human plasma. The
high performance liquid chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the
human plasma comprises the following steps that: (1) sample pre-treatment is conducted, interior
label working solution and spiritus mindereri are successively added into a
plasma sample, vortex is mixed, and
centrifugation is conducted; (2) weak positive
ion exchange
solid phase extraction 96 pore plate (SPE 96 poreplate) is activated; (3)
solid phase extraction treatment is conducted, after the step (1) is conducted, the sample is loaded into the treated SPE 96 pore plate, a
formic acid acetonitrile solution is eluted, and eluent is dried by
nitrogen; and (4) redissolution is conducted, analysis and detection are conducted by the
high performance liquid chromatography detection method. The high performanceliquid
chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the
human plasma has the advantages that accuracy is high, reproducibility is high, sensitivityis high, specificity is high, the efficiency is high, flux is high, the cost is low, and the operation is easy, convenient and fast.