Novel method for simultaneously measuring contents of multiple active ingredients of dogwood

Abstract

The invention discloses a novel method for simultaneously measuring contents of multiple active ingredients of dogwood. The method is used for simultaneously measuring the contents of seven ingredients, namely loganin, gallic acid, 5-HMF (hydroxymethyl furfural), protocatechuic acid, morroniside, sweroside and cor-nuside, in dogwood medicinal materials from four main producing areas by utilizing a high performance liquid diode array detection method. The method comprises the steps of: carrying out gradient elution at the flow speed of 1.0 mL.min-1 by utilizing a Phenoemenex C18 polar chromatographic column and taking acetonitrile and 0.1% methanoic acid solution as mobile phases, wherein the column temperature is 30 DEG C, the detection wavelengths are 220 nm, 240 nm, 260 nm, 265 nm and 280 nm. The contents of the loganin, the gallic acid, the 5-HMF, the protocatechuic acid, the morroniside, the sweroside and the cor-nuside in each sample are respectively 0.22-0.800 mg / g, 0.002-0.024 mg / g, 0.069-3.775 mg / g, 8.192-28.111 mg / g, 0.048-0.598 mg / g, 1.228-9.311 mg / g and 1.450-8.381 mg / g. The method is simple and accurate, is good in reproducibility and is capable of providing bases for comprehensively evaluating and controlling the quality of dogwood decoction pieces.

Description

technical field [0001] The invention belongs to the technical field of chemistry, and relates to a new method for simultaneously measuring the contents of multiple active ingredients in Cornus officinalis. Background technique [0002] Cornus officinalis is the dried and mature pulp of Cornus officinalis Sieb.Et Zucc. Dogwood is mainly produced in Shaanxi, Henan, Zhejiang and other provinces in my country, and it is also cultivated in Sichuan, Anhui, and Shandong. Cornus officinalis has a medicinal history of 2,000 years. It was first recorded in "Shen Nong's Materia Medica" and listed as a middle grade. "Chinese Pharmacopoeia One" (2010 edition) records that it can nourish the liver and kidney, reduce astringency and solidify, and is used for dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, enuresis, frequent urination, uterine bleeding, profuse sweating, prostration, internal heat and quenching thirst . Modern studies have proved that Cornus offi...

AI Technical Summary

Problems solved by technology

At present, the quality control of Cornus officinalis in the 2010 edition of the Pharmacopoeia is relatively simple, and only the content of loganin can not be comprehensively evaluated for the quality of today’s complicated Cornus officinalis. Ingredients, such as gallic acid, 5-hydroxymethylfurfural (5-HMF), protocatechuic acid, morroniside, swertiroside, loganin and cornel glycosides and other 7 active ingredients, can be more effective Comprehensive and Accurate Evaluation of the Quality of Cornus officinalis

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