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Extracts of total saponins of pterocephalus hookeri and preparation method and application thereof

The technology of a glycoside extract and S. serrata is applied in the field of total glycosides extract of S. serrata and its preparation field, and the effect of exact curative effect is achieved.

Inactive Publication Date: 2012-07-18
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the existing literature, it can be known that only the total saponins and loganin in Pterocephalus have anti-inflammatory and analgesic effects, and the anti-tumor effects of total saponins have been reported, and no total glycosides extracts have been reported to treat rheumatoid arthritis. Related reports on the active ingredients

Method used

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  • Extracts of total saponins of pterocephalus hookeri and preparation method and application thereof
  • Extracts of total saponins of pterocephalus hookeri and preparation method and application thereof
  • Extracts of total saponins of pterocephalus hookeri and preparation method and application thereof

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Embodiment 1

[0025] Embodiment 1 The preparation of the total glycosides of pterygium of the present invention

[0026] Take 1000g of the prairie herb and cut it into sections, add 1600ml of water to moisten it, decoct in 6000ml of water to extract twice, 1.5h each time, filter, after the filtrate is concentrated, centrifuge to remove the precipitate, and dilute the water extract to 0.1g crude drug / ml drug solution, using D101 type macroporous adsorbent resin to load at room temperature, the flow rate of sample loading is 2BV / h, the sample volume is 1 times of the amount of resin (medicine: resin=1:1), eluted with 4BV water, and the flow rate is 2BV / h, discard the eluent; elute with 3BV of 70% ethanol, the elution flow rate is 3BV / h, the eluent is decompressed to recover ethanol to a thick paste, dry at low temperature or freeze-dry, crush, and pass through a No. 4 sieve , to obtain the total glycoside extract.

Embodiment 2

[0027] The content detection of embodiment 2 total glycosides of pterygium

[0028] 1. Determination of the content of total glycosides in the extract

[0029] Measured according to the colorimetric method (Appendix VA of the 2010 edition of the Pharmacopoeia of the People's Republic of China).

[0030] Preparation of reference substance solution: Take an appropriate amount of loganin reference substance, weigh it accurately, add distilled water to make a solution containing 6mg per 100ml, and obtain it.

[0031] Preparation of standard curve: Take an appropriate amount of loganin reference substance, weigh it accurately, add methanol to make a 60 μg / ml solution. Accurately measure 0.6ml, 0.8ml, 1.0ml, 1.2ml, 1.4ml, and 1.6ml of the reference solution respectively into colorimetric tubes, evaporate the solvent, and let cool. Add 0.2ml of freshly prepared 5% vanillin-glacial acetic acid solution and 0.8ml of perchloric acid in sequence, heat in a water bath at 60°C for 15 min...

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Abstract

The invention provides extracts of total saponins of pterocephalus hookeri. The extracts are characterized in that the extracts root in dry whole grass of dipsacaceae species pterocephalus hookeri (C.B.Clarke) Hoeck; in the extracts, the total content of total saponins and total iridoid glycoside is not lower than 50%w / w; and the extracts contain 0.45-0.78%w / w of loganin, 0.2-0.45%w / w of oleanolic acid and 0.65-1.1%w / w of ursolic acid. The invention also provides a preparation method and application of the extracts. The extracts have good anti-inflammatory and analgesic effects, have a certain effect on inhibiting specific cellular immunity of organisms, can effectively treat rheumatoid arthritis by reducing the proinflammatory factor level of rheumatoid arthritis and improving the oxidation resistance of the organisms, have extract curative effect and provide a new choice for clinical medication.

Description

technical field [0001] The invention relates to a total glycosides extract of pterygium and its preparation method and application, belonging to the field of medicine. Background technique [0002] Rheumatoid arthritis (RA) is a common systemic immune disease characterized by chronic polyarthritis, with hundreds of millions of patients worldwide. According to statistics, the prevalence rate of RA in foreign countries is 1-2%, and it is as high as 5% in some regions; the incidence rate in my country is about 0.74%, and there are about 10 million patients. According to the investigation, in the normal population, the incidence of rheumatoid arthritis accounts for about 0.35% of the normal population. The disability rate of this disease is high, and the disability rate of patients with rheumatoid arthritis who is not diagnosed and treated in time is 50% in two years and 70% in three years. The average life expectancy of patients with rheumatoid arthritis is shortened by 10 to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/02A61P29/00A61K31/7048A61K31/56
Inventor 张艺孟宪丽赖先荣古锐
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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