Aquilaria sinensis leaf extract as well as preparation method and application thereof

Abstract

The invention relates to the field of traditional Chinese medicine extraction, and particularly discloses an aquilaria sinensis leaf extract as well as a preparation method and an application thereof. The extract comprises the following six components: 2-O-a-rhamnoside-4,6,4'-trihydroxybenzo phenone, 3-C-heteroside-2,4,6,4'-tetrahydroxybenzophenone, mangiferin, 3.5-C-glucoside-2,4,6,4'-trihydroxybenzo phenone, 5,4-dimethoxy-7-hydroxy flavone and apione-7,4'-dimethyl ether. The aquilaria sinensis leaf extract has good anti-inflammatory and analgesic effects and can be used for preparing anti-inflammatory and analgesic drugs; and the effective constituents in the extract are clear so that benefit is brought to the medication safety.

Description

technical field

[0001] The invention relates to the field of extraction of traditional Chinese medicines, in particular to an extract of arborina japonica leaves and a preparation method and application thereof. Background technique

[0002] White wood incense is commonly called earth agarwood, and it is mainly produced in Southeast Asian countries such as India and Malaysia. Rich in the Lingnan area of ​​my country, it is a rare and precious Chinese medicinal material with a long history in my country, and is also an important "Southern Medicine" resource. It ranks among the "Top Ten Southern Medicines". Aquilaria sinensis (Lour.) Gilg.] The resinous part of the heartwood. In modern clinics, it is often used in the treatment of various diseases mainly involving the digestive system, nervous system and cardiovascular system, and has good results. All editions of the "Chinese Pharmacopoeia" have recorded agarwood, and since the 2010 edition of the Pharmacopoeia, agarwood ha...

Examples

Embodiment 1

[0025] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume (60% ethanol) to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0026] After testing, the extract contains 6% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 5% of 3-C-glucoside-2,4,6, 4'.-tetrahydroxybenzophenone, 40% mangiferin, 6% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone, 14% 5,4 -Dimethoxy-7-hydroxyflavone, 13% acetin-7,4'-dimethyl ether.

[0027] The detection method of above-mentioned each compositio...

Embodiment 2

[0031] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume of 50% ethanol to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0032] After testing (the detection method is the same as in Example 1), the extract contains 8% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 10% of 3-C-glucose Glycoside-2,4,6,4'.-tetrahydroxybenzophenone, 45% mangiferin, 8% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone Ketone, 12% 5,4-dimethoxy-7-hydroxyflavone, 10% apigenin-7,4'-dimethyl ethe...

Embodiment 3

[0034] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume (40% ethanol) to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0035] After testing (the detection method is the same as in Example 1), the extract contains 10% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 12% of 3-C-glucose Glycoside-2,4,6,4'.-tetrahydroxybenzophenone, 52% mangiferin, 9% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone Ketones, 4% 5,4-dimethoxy-7-hydroxyflavone, 6% apigenin-7,4'-dimethyl ether...

technical field

[0001] The invention relates to the field of extraction of traditional Chinese medicines, in particular to an extract of arborina japonica leaves and a preparation method and application thereof. Background technique

[0002] White wood incense is commonly called earth agarwood, and it is mainly produced in Southeast Asian countries such as India and Malaysia. Rich in the Lingnan area of ​​my country, it is a rare and precious Chinese medicinal material with a long history in my country, and is also an important "Southern Medicine" resource. It ranks among the "Top Ten Southern Medicines". Aquilaria sinensis (Lour.) Gilg.] The resinous part of the heartwood. In modern clinics, it is often used in the treatment of various diseases mainly involving the digestive system, nervous system and cardiovascular system, and has good results. All editions of the "Chinese Pharmacopoeia" have recorded agarwood, and since the 2010 edition of the Pharmacopoeia, agarwood ha...

Examples

Embodiment 1

[0025] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume (60% ethanol) to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0026] After testing, the extract contains 6% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 5% of 3-C-glucoside-2,4,6, 4'.-tetrahydroxybenzophenone, 40% mangiferin, 6% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone, 14% 5,4 -Dimethoxy-7-hydroxyflavone, 13% acetin-7,4'-dimethyl ether.

[0027] The detection method of above-mentioned each compositio...

Embodiment 2

[0031] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume of 50% ethanol to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0032] After testing (the detection method is the same as in Example 1), the extract contains 8% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 10% of 3-C-glucose Glycoside-2,4,6,4'.-tetrahydroxybenzophenone, 45% mangiferin, 8% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone Ketone, 12% 5,4-dimethoxy-7-hydroxyflavone, 10% apigenin-7,4'-dimethyl ethe...

Embodiment 3

[0034] Weigh 1 kg of dry Akiras sinensis leaf powder and place them in a round-bottomed flask, add 10 times the amount of 70% ethanol, reflux 3 times, 1 hour each time, filter, combine the filtrate, and concentrate the filtrate until it has no alcohol smell; pass through AB- 8 macroporous resin columns, control the flow rate to 2BV / h, first use 5 times the column volume (5BV) of water to elute the macroporous resin to remove impurities; then use 3 times the column volume (40% ethanol) to elute the macroporous resin, collect Concentrate and dry the part eluted with ethanol to obtain the extract of Akiras sinensis leaves.

[0035] After testing (the detection method is the same as in Example 1), the extract contains 10% of 2-O-a-rhamnoside-4,6,4'.-trihydroxybenzophenone, 12% of 3-C-glucose Glycoside-2,4,6,4'.-tetrahydroxybenzophenone, 52% mangiferin, 9% 3.5-C-glucoside-2,4,6,4'.-tetrahydroxybenzophenone Ketones, 4% 5,4-dimethoxy-7-hydroxyflavone, 6% apigenin-7,4'-dimethyl ether...

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