149 results about "Oleanonic acid" patented technology

The invention discloses pulsatilla saponin B4-oleanolic acid self-assembled nanoparticles as well as preparation and application thereof, and relates to the technical field of pharmaceutical preparations. Inspired by compatibility and solubilization of traditional Chinese medicines, the pulsatilla saponin B4-oleanolic acid self-assembled nanoparticles (AB4-OA NPs) are prepared by taking natural active components, namely micromolecular pulsatilla saponin B4 and oleanolic acid, as raw materials; the oleanolic acid has the effects of promoting dissolution and resisting inflammation, the water solubility of the oleanolic acid is improved, the bioavailability is improved, and the anti-inflammatory effect of the oleanolic acid is enhanced. Experimental results show that the AB4-OA NPs can effectively improve the dissolution rate of the oleanolic acid, so that the bioavailability of the oleanolic acid is effectively improved, meanwhile, the AB4-OA NPs has a better anti-inflammatory effect, and the effect is better than that of a single drug with the same dosage. In addition, the synthetic preparation method is simple and convenient, has the advantages of nano-scale drugs, realizes auxiliary-material-free pure drug self-delivery, and improves the delivery efficiency.

The present invention relates to a method of treating a glucose-6-phosphate dehydrogenase dysregulated disorder in a subject in need thereof, the method comprising administering to the subject an effective amount of oleanolic acid, a conjugated salt thereof, or a prodrug thereof. The glucose 6-phosphate dehydrogenase dysregulated disorder can be myofibrillar myopathy, amyotrophic lateral sclerosis, Huntington's disease, Parkinson's disease and Alzheimer's disease caused by BCL2 associated immortal gene mutation.

To provide a method for producing an oleanolic acid-containing extract which enables easy production of an oleanolic acid-containing extract from grape pomace without the need of using high-concentration organic solvents.SOLUTION: This method includes: a first step of preparing a mixed solution containing a natural deep eutectic solvent with sugar beet-derived betaine as the hydrogen bond acceptor and grape pomace; and a second step of deriving an extract containing an oleanolic acid from the mixed solution prepared in the first step.SELECTED DRAWING: None

The invention discloses traditional Chinese medicine monomer self-assembled nanoparticles as well as a preparation method and application thereof, and relates to the technical field of pharmaceutical preparations. According to the prepared traditional Chinese medicine monomer self-assembled nanoparticles, namely ginsenoside Rg1-oleanolic acid self-assembled nanoparticles, the self-assembled structural characteristics of ginsenoside Rg1 are ingeniously utilized, the superficial area of the medicine can be effectively increased, the solubility of oleanolic acid can be improved, and therefore the bioavailability of oleanolic acid is effectively improved, and the anti-inflammatory effect of the oleanolic acid is further enhanced. And compared with a physical mixed medicine with the same dosage, the effect is more remarkable. Besides, the preparation method is simple and easy to implement, not only has the advantages of nanoscale drugs, but also realizes self-delivery of pure drugs without auxiliary materials, and greatly improves the delivery efficiency of the drugs.

The invention provides a dual-targeting carrier material, a preparation method thereof and a preparation method of oleanolic acid nanoparticles wrapped by the dual-targeting carrier material, and belongs to the technical field of medicines. The oleanolic acid targeting nanoparticles are prepared by taking a dual-targeting high-molecular copolymer as a carrier material and adopting an emulsification-solvent evaporation method, so that the oleanolic acid can be protected from being quickly degraded, and the in-vivo circulation time of the oleanolic acid can be prolonged. Meanwhile, targeted delivery of oleanolic acid is realized through a targeted group, the concentration of the drug in tumor tissues is improved, and toxic and side effects on normal tissues are reduced. An MTT method is adopted, the inhibition effect of the oleanolic acid nanoparticles on cell growth is researched, and the result shows that the oleanolic acid targeting nanoparticles have a stronger effect of inhibiting tumor cell proliferation compared with an oleanolic acid raw material medicine.

The invention discloses an oleanolic acid / cyclodextrin metal organic framework compound and a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: dissolving gamma-cyclodextrin and alkaline potassium salt in water to obtain crystallization mother liquor of a cyclodextrin metal organic framework; and then adding oleanolic acid into the crystallization mother liquor of the cyclodextrin metal organic framework for co-crystallization growth to obtain the oleanolic acid / cyclodextrin metal organic framework compound. According to the co-crystallization method provided by the invention, the oleanolic acid can be efficiently encapsulated in the cyclodextrin metal organic framework, and the solubility of the oleanolic acid is improved by 109.81 times by the prepared oleanolic acid / cyclodextrin metal organic framework compound, so that the lipid-lowering activity of the oleanolic acid is further enhanced; the compound provided by the invention has wide application potential in the aspects of preparing medicines for preventing / treating the non-alcoholic fatty liver disease and the like.

The invention discloses matrine-oleanolic acid ionic liquid and a preparation method and application thereof.The preparation method comprises the steps that matrine and oleanolic acid are dissolved in an organic solvent, heating and stirring are conducted, then rotary evaporation is conducted, and the matrine-oleanolic acid ionic liquid is obtained; wherein the mass ratio of the sum of the matrine and the oleanolic acid to the organic solvent is 1: (5-30), and the molar ratio of the matrine to the oleanolic acid is (3-10): 1. The ionic liquid disclosed by the invention not only can effectively improve the water solubility of oleanolic acid, but also can effectively inhibit the activity of 5 alpha-reductase and prevent the 5 alpha-reductase from catalyzing testosterone to be converted into DHT, so that the problems of rapid entry into a retrogression period, miniaturization, hair loss and the like caused by the fact that DHT acts on hair follicles after being combined with an androgen receptor are prevented. Meanwhile, expression of IL-1beta and TNF-alpha inflammatory factors in skin hair follicles can be inhibited, and the purposes of preventing alopecia and promoting hair growth are achieved. The effects of controlling oil, resisting inflammation, relieving, removing dandruff, relieving itching and the like are also achieved.

The invention belongs to the field of biosynthesis, and particularly relates to a saccharomyces cerevisiae engineering bacterium for producing glycosyl donor UDP-arabinose as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. According to the invention, UDP-Glc dehydrogenase, UDP-GlcA decarboxylase, UDP-Xyl-4-epimerase or UDP-Glc-epimerase are sequentially expressed in WAT11, so that a synthetic route of UDP-Ara is introduced into the saccharomyces cerevisiae for the first time; de novo synthesis of Ara-oleanolic acid is realized by introducing a synthetic route of arabinosyl transferase and oleanolic acid; the yeast containing the UDP-Ara synthetic pathway and the arabinosyl transferase gene can also utilize betulinic acid to generate Ara-betulinic acid. The invention solves the problem of yeast total synthesis of the arabinosyl donor, provides a new biotechnology platform for production of natural glycoside compounds, and has important theoretical value and wide application prospect.

An aqueous oleanolic acid solution, including from about 0.10 to 2.5 weight % oleanolic acid, from about 60 to 90 weight % solvent, and from about 20 to 40 weight % solubility agent, wherein the solvent includes water, the solubility agent includes sodium lauryl sulfate (SLS), and the aqueous oleanolic acid solution is substantially free of ethanol and dimethyl sulfoxide (DMSO).

The invention discloses an anti-hair loss hair care component, a preparation method of the anti-hair loss hair care component and shampoo. The anti-hair loss hair care component is ionic salt formed by R-Lys-Gly-His-Lys-NH2 and oleanolic acid, wherein R is palmitoyl or myristoyl; the composition has high-temperature storage stability, transdermal property and HDPCs proliferation promotion effect in an aqueous solution environment, so that hair loss prevention and hair growth are promoted. The ionic salt is prepared in an atomization mixing mode, and the method has the advantages of being high in product purity, high in reaction efficiency and simple in process. The invention also provides a shampoo containing the anti-hair loss and hair care component, and the shampoo has proper viscosity and pH value.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for treating atopic dermatitis and application thereof. The composition comprises the following components: pseudo-ginseng extract, artemether, alpine glycoside and oleanolic acid in a mass ratio of (2-4): (2-4): (1-2): (1-2). The composition disclosed by the invention has anti-inflammatory and anti-pruritus effects and can be used for treating atopic dermatitis, eczema and other diseases. The active components of the composition are all from plant sources, the composition has good safety and atopic dermatitis treatment efficacy, the use dosage is low, the components can achieve a synergistic interaction effect, and the composition shows an excellent effect; the composition can be applied to functional skin care products (face cream, emulsion and essence) and external medicines (applying agents and ointments).

The invention relates to the technical field of agriculture, and discloses an ecological environment-friendly microbial degradation mulching film which comprises the following components in percentage by mass: 50-65% of polylactic acid; 15% to 25% of lignocellulose; 5%-10% of glycerin; 0.5%-2% of nano titanium dioxide; 0.1% to 0.5% of silver nanoparticles; 0.5%-2% of a tea tree oil extract; 15% to 20% of carboxymethyl cellulose; 13%-15% of a plant growth regulator; 5%-10% of an auxiliary agent; the auxiliary agent comprises one or more of an antioxidant, a plasticizer and a light stabilizer, and the plant growth regulator comprises one or more of a seaweed extract, indoleacetic acid or oleanolic acid. By adding nano titanium dioxide and silver nanoparticles into the mulching film, excellent ultraviolet resistance and antibacterial performance of the mulching film are achieved, and the problem that aging of a traditional mulching film is accelerated due to ultraviolet irradiation is solved.

The invention provides a wool fabric anti-pilling finishing agent and a preparation method, and relates to the field of fabric finishing agents, the wool fabric anti-pilling finishing agent comprises a dry material and a wet material, the dry material is obtained by adding a seaweed extract and oleuropein into a mixture of sericin and water and performing microwave treatment, low-temperature drying treatment and crushing treatment on the mixture, and the wet material is obtained by adding the seaweed extract and the oleuropein into the mixture. The wet material is obtained by mixing tea polyphenol, oleanolic acid and water. The anti-pilling finishing agent for the wool fabric, provided by the invention, is prepared from the sericin, the seaweed extract, the oleuropein, the tea polyphenol and the oleanolic acid materials, and the application of the natural materials not only is environment-friendly, but also can enhance the mechanical properties of the wool fabric while improving the anti-pilling property and the antistatic property of the wool fabric; after the anti-pilling finishing agent for the wool fabric is applied to the wool fabric, the wool fabric has the anti-pilling property and the durability.

The invention discloses a soothing and repairing gentiana macrophylla extract composition as well as a preparation method and application thereof, and particularly belongs to the technical field of preparation of raw materials of daily necessities. The gentiana macrophylla extract composition is obtained by performing compound solid-state fermentation on gentiana macrophylla through aspergillus oryzae, leuconostoc citreum and saccharomyces cerevisiae and then performing solvent extraction. The gentiana macrophylla extract composition provided by the invention has relatively high oleanolic acid content, which proves that the aspergillus oryzae, the leuconostoc citreum and the saccharomyces cerevisiae can effectively enrich active ingredients in gentiana macrophylla, and meanwhile, the aspergillus oryzae, the leuconostoc citreum and the saccharomyces cerevisiae have a certain synergistic effect on the enrichment of the active ingredients in the fermentation process; the gentiana macrophylla extract composition provided by the invention can effectively inhibit release of inflammatory factors and promote skin repair.

A use of oleanolic acid-28-O-β-D-glucopyranoside in the preparation of an anti-colitis drug is provided. The oleanolic acid-28-O-β-D-glucopyranoside has significant anti-ulcerative-colitis activity compared with parent nucleus oleanolic acid and other analogues, and has equivalent efficacy compared with Mesalazine, a first-line drug for the clinical treatment of inflammatory bowel disease, and can also avoid salicylic acid anaphylaxis possibly caused by Mesalazine western medicine treatment, thereby achieving a good application prospect.

The invention relates to the technical field of dressings, and discloses a dressing with a skin repair function and a production process thereof, the dressing with the skin repair function comprises the following raw materials: antibacterial cellulose, anti-inflammatory repair type carrageenan, diallyldimethylammonium chloride, an initiator and deionized water; wherein the antibacterial cellulose is prepared from bacterial cellulose and oleanolic acid; the anti-inflammatory repair type carrageenan is prepared from alkenyl carrageenan and baicalin; the alkenyl carrageenan is prepared from carrageenan and glycidyl methacrylate; the dressing prepared by the invention has an excellent and long-acting antibacterial effect, can effectively inhibit the reproduction of bacteria in a moist environment, provides a good living environment for repairing skin cells, does not easily cause inflammation, avoids scars, accelerates the wound healing rate, and can effectively promote the regeneration and repairing of the skin cells.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of oleanolic acid in promoting chondrogenic differentiation of chondrocytes. This invention discovers that oleanolic acid promotes the expression of proteoglycans and calcium deposition in ATDC5 cells undergoing chondrogenic differentiation, and also promotes the expression of ATDC5 cell chondrogenic differentiation markers. Therefore, it has the potential to be formulated into a drug that can promote chondrogenic differentiation of chondrocytes, thereby promoting cartilage repair and regeneration for the treatment of osteoarthritis and rheumatoid arthritis.

The present invention discloses loquat triterpenic acid synthesis key enzyme genes EjCYP716A1 / 2 and EjCYP716C1 / 2 and applications. The present invention first reveals the function of a new oxidized squalene cyclase gene EjOSC2 in synthesizing α / β-amyrin in the process of triterpenic acid synthesis; clarifies the function of gene EjCYP716A1 / 2 in catalyzing oxidation reaction at C-28 of α / β-amyrin to convert it into ursolic acid and oleanolic acid; and clarifies the function of EjCYP716C1 / 2 in catalyzing hydroxylation reaction at C-2 of ursolic acid / oleanolic acid to convert it into corosolic acid / maslinic acid. These five genes can provide gene resources for regulating the accumulation level of ursane-type and oleanolic acid-type triterpenic acids in tissues such as leaves of medicinal plants loquat, and also lay the foundation for batch production of natural active triterpenic acid components such as loquat ursolic acid and corosolic acid through environmentally friendly production processes such as gene editing or fermentation engineering.

The present application relates to the technical field of oyster peptides, and specifically to a method for preparing oyster peptides and their use in improving male reproductive function. The present application utilizes oyster peptides as raw materials, grafts oleanolic acid saponins thereon, and composites them with chitosan to prepare an emulsion of a ternary complex. The emulsion can form stable microscopic particles and has stable rheological properties. Through simulated digestion experiments, it was found that the emulsion has a sustained-release effect on the digestion of oyster peptides, and can at least reduce the proportion of digestion in the oral cavity. In addition, the use of the emulsion and the oyster peptide to intervene in rats with nephrotic syndrome can effectively alleviate their nephrotic syndrome, and at the same time promote the male sexual function of rats, and has an application prospect of modifying and improving male reproductive function.

The invention relates to the cosmetic and non-therapeutic use of a composition consisting essentially of a compound of formula I below: (I) or its enantiomers, isomers or diastereoisomers, or a mixture thereof, or a salt or a solvate thereof, wherein R1 and R2, independently of each other, are a C3-C6 sugar, a uronic acid, or a glucoside, for bleaching or whitening the skin and skin appendages of a healthy individual.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The invention discloses a pharmaceutical composition for inducing leukemia cell copper death and application thereof, and relates to the technical field of tumor cell apoptosis. The active ingredients of the pharmaceutical composition are oleanolic acid and illlisemom. It is found for the first time that oleanolic acid can significantly up-regulate expression of a copper death key regulatory factor FDX1, when oleanolic acid is used in combination with a copper ion carrier Ilismor, a large synergistic effect can be generated through an FDX1 / DLAT axis, leukemia cells are significantly induced to generate copper death, and therefore in-vitro proliferation and in-vivo tumor forming ability of the leukemia cells are effectively inhibited. The composition provides a new drug development strategy with a clear mechanism for treatment of leukemia, especially drug-resistant leukemia.

The application discloses a method for preparing oleanolic acid-beta-D-glucopyranosyl ester from makaline, and relates to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: (1) makaline is extracted by using a solvent to obtain an extraction solution, and then solvent evaporation treatment is performed to obtain a first extract; (2) the first extract is preliminarily separated by using a macroporous resin chromatography, elution is performed by using different gradient concentrations of ethanol solution, 20% (volume concentration) ethanol eluent is obtained, and then solvent evaporation treatment is performed to obtain a second extract; and (3) the second extract is separated by using a high-speed counter-current chromatography, a fraction corresponding to a first sample peak is collected, and then drying treatment is performed, so that the oleanolic acid-beta-D-glucopyranosyl ester is obtained. The method provides a new approach for the separation and preparation of the oleanolic acid-beta-D-glucopyranosyl ester, and the method is simple, fast and high in preparation purity.

The application discloses application of oleanolic acid in promoting colonization of probiotic EcN strain in a gastrointestinal tract, and the oleanolic acid can promote formation of a biofilm of the probiotic EcN, enhance hydrophobicity of a surface of the probiotic EcN strain, and inhibit movement ability of the EcN strain, thereby benefiting adhesion and aggregation of the EcN strain, and further stabilizing colonization and survival in the intestinal tract, and having potential to be developed into probiotic food and preparations.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an inhalable powder preparation based on screened lactose and a preparation method of the inhalable powder preparation. The inhalable powder preparation is prepared from the following raw materials in parts by weight: 1 to 1.25 parts of active pharmaceutical ingredients, 55 to 60 parts of screened lactose, 5 to 12 parts of modified lactose fine powder, 1 to 4 parts of hydroxypropyl-beta-cyclodextrin and 1 to 3 parts of oleanolic acid beta-D-glucopyranosyl ester. The modified lactose fine powder is palmitoyl chloride grafted and modified lactose fine powder, and the modification treatment can weaken the formation ability of a hydration layer on the surface of lactose, reduce the surface polarity and generate steric hindrance, so that the self-agglomeration tendency among the lactose fine powder is remarkably reduced, and the binding characteristic of the lactose fine powder and medicine particles is improved; furthermore, the dispersing performance and the lung deposition efficiency of the preparation are remarkably improved. In addition, the hydroxypropyl-beta-cyclodextrin and the oleanolic acid beta-D-glucopyranosyl ester are matched for use, so that the stability of the preparation can be remarkably improved.

This invention provides a method for preparing camellia oil rich in oleanolic acid, comprising the following steps: (1) washing and draining fresh camellia fruit shells and / or tea leaves, then pulverizing them after vacuum freeze-drying to obtain freeze-dried powder A, and pulverizing dried camellia seed kernels to obtain seed kernel powder B. (2) mixing freeze-dried powder A and seed kernel powder B at a mass ratio of 1:(15-20). (3) adding a 25%-35% ethanol aqueous solution as an extraction solvent to the mixed raw materials, wherein the mass-volume ratio (g:mL) of the mixed raw materials to the extraction solvent is 1:(3-4); using a high-speed shearing device to vigorously stir and crush the mixed system for 3-10 minutes to achieve full cell wall disruption of the plant cells. (4) heating and reflux extraction; (5) three-phase separation and solvent reuse. This method has a high efficiency in transferring oleanolic acid from the raw materials to the oil phase.

The application relates to the field of cosmetics, and relates to an anti-aging cosmetic, grape pomace oil and a preparation method thereof. The anti-aging cosmetic comprises grape pomace oil; characteristic components of the grape pomace oil include oleanolic acid, alpha-guaiacwoodene, beta-tocotrienol, beta-sitosterol, campesterol, soybean sapogenol E, ganoderic acid F, ganoderol I and D-erythro-dihydrosphingosine; the total phenol content of the grape pomace oil is greater than or equal to 1 mg / g, the total sterol content of the grape pomace oil is greater than or equal to 26 mg / g, and the total flavonoid content of the grape pomace oil is greater than or equal to 1 mg / g; the grape pomace oil is extracted from grape pomace; the grape pomace is mainly composed of grape skin, grape stem and grape seed. The compounds have excellent antioxidant properties and can improve the anti-aging effect of the anti-aging cosmetic. The compounds are helpful for protecting the skin from oxidative damage and play a positive role in cosmetics. The grape pomace oil maximally retains active components of all parts of the grape pomace.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The present invention provides an animal feed additive with anti-inflammatory and antioxidant functions, namely, a traditional Chinese medicine-type feed additive with anti-biotic efficacy. The preparation method comprises the following steps: mixing a dried olive pomace powder sample with a subcritical extractant, performing subcritical extraction, and obtaining an extraction system; removing the subcritical extractant from the extraction system to obtain a pomace powder system from which pomace oil has been removed; mixing the pomace powder system with ethanol, performing leaching, and subjecting the obtained extract to vacuum distillation to obtain an extract; mixing the extract system with petroleum ether, allowing the petroleum ether phase to stand for stratification, and filtering the obtained petroleum ether phase to obtain an extract. The maslinic acid and oleanolic acid extracted by the present invention have anti-inflammatory and antioxidant effects, and animal experiments have confirmed that they can enhance intestinal health in a short period of time. The addition of a polypeptide improves the side effects of the maslinic acid and oleanolic acid caused by their long-term use. The present invention can be promoted and used as a new animal feed additive.

The invention discloses a pH-responsive HG type pectin hydrogel based on Schiff base reaction and a preparation method and application thereof, the pH-responsive HG type pectin hydrogel has a structural formula as shown in the following formula I. According to the pH-responsive HG type pectin hydrogel, functional characteristics of HG type pectin, biological activity of oleanolic acid, 3, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3- The intelligent hydrogel system with ROS / pH dual response characteristics is successfully constructed through the redox responsiveness of oleanolic acid, 3, 3 '-dithiodipropionic acid and the pH responsiveness of Schiff base reaction, and the system can effectively protect the activity of oleanolic acid and can be used for preparing drugs for improving alcoholic liver injury.