98 results about "Oleanonic acid" patented technology

The invention discloses matrine-oleanolic acid ionic liquid and a preparation method and application thereof.The preparation method comprises the steps that matrine and oleanolic acid are dissolved in an organic solvent, heating and stirring are conducted, then rotary evaporation is conducted, and the matrine-oleanolic acid ionic liquid is obtained; wherein the mass ratio of the sum of the matrine and the oleanolic acid to the organic solvent is 1: (5-30), and the molar ratio of the matrine to the oleanolic acid is (3-10): 1. The ionic liquid disclosed by the invention not only can effectively improve the water solubility of oleanolic acid, but also can effectively inhibit the activity of 5 alpha-reductase and prevent the 5 alpha-reductase from catalyzing testosterone to be converted into DHT, so that the problems of rapid entry into a retrogression period, miniaturization, hair loss and the like caused by the fact that DHT acts on hair follicles after being combined with an androgen receptor are prevented. Meanwhile, expression of IL-1beta and TNF-alpha inflammatory factors in skin hair follicles can be inhibited, and the purposes of preventing alopecia and promoting hair growth are achieved. The effects of controlling oil, resisting inflammation, relieving, removing dandruff, relieving itching and the like are also achieved.

An aqueous oleanolic acid solution, including from about 0.10 to 2.5 weight % oleanolic acid, from about 60 to 90 weight % solvent, and from about 20 to 40 weight % solubility agent, wherein the solvent includes water, the solubility agent includes sodium lauryl sulfate (SLS), and the aqueous oleanolic acid solution is substantially free of ethanol and dimethyl sulfoxide (DMSO).

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for treating atopic dermatitis and application thereof. The composition comprises the following components: pseudo-ginseng extract, artemether, alpine glycoside and oleanolic acid in a mass ratio of (2-4): (2-4): (1-2): (1-2). The composition disclosed by the invention has anti-inflammatory and anti-pruritus effects and can be used for treating atopic dermatitis, eczema and other diseases. The active components of the composition are all from plant sources, the composition has good safety and atopic dermatitis treatment efficacy, the use dosage is low, the components can achieve a synergistic interaction effect, and the composition shows an excellent effect; the composition can be applied to functional skin care products (face cream, emulsion and essence) and external medicines (applying agents and ointments).

The invention provides a wool fabric anti-pilling finishing agent and a preparation method, and relates to the field of fabric finishing agents, the wool fabric anti-pilling finishing agent comprises a dry material and a wet material, the dry material is obtained by adding a seaweed extract and oleuropein into a mixture of sericin and water and performing microwave treatment, low-temperature drying treatment and crushing treatment on the mixture, and the wet material is obtained by adding the seaweed extract and the oleuropein into the mixture. The wet material is obtained by mixing tea polyphenol, oleanolic acid and water. The anti-pilling finishing agent for the wool fabric, provided by the invention, is prepared from the sericin, the seaweed extract, the oleuropein, the tea polyphenol and the oleanolic acid materials, and the application of the natural materials not only is environment-friendly, but also can enhance the mechanical properties of the wool fabric while improving the anti-pilling property and the antistatic property of the wool fabric; after the anti-pilling finishing agent for the wool fabric is applied to the wool fabric, the wool fabric has the anti-pilling property and the durability.

A use of oleanolic acid-28-O-β-D-glucopyranoside in the preparation of an anti-colitis drug is provided. The oleanolic acid-28-O-β-D-glucopyranoside has significant anti-ulcerative-colitis activity compared with parent nucleus oleanolic acid and other analogues, and has equivalent efficacy compared with Mesalazine, a first-line drug for the clinical treatment of inflammatory bowel disease, and can also avoid salicylic acid anaphylaxis possibly caused by Mesalazine western medicine treatment, thereby achieving a good application prospect.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of oleanolic acid in promoting chondrogenic differentiation of chondrocytes. This invention discovers that oleanolic acid promotes the expression of proteoglycans and calcium deposition in ATDC5 cells undergoing chondrogenic differentiation, and also promotes the expression of ATDC5 cell chondrogenic differentiation markers. Therefore, it has the potential to be formulated into a drug that can promote chondrogenic differentiation of chondrocytes, thereby promoting cartilage repair and regeneration for the treatment of osteoarthritis and rheumatoid arthritis.

The present application relates to the technical field of oyster peptides, and specifically to a method for preparing oyster peptides and their use in improving male reproductive function. The present application utilizes oyster peptides as raw materials, grafts oleanolic acid saponins thereon, and composites them with chitosan to prepare an emulsion of a ternary complex. The emulsion can form stable microscopic particles and has stable rheological properties. Through simulated digestion experiments, it was found that the emulsion has a sustained-release effect on the digestion of oyster peptides, and can at least reduce the proportion of digestion in the oral cavity. In addition, the use of the emulsion and the oyster peptide to intervene in rats with nephrotic syndrome can effectively alleviate their nephrotic syndrome, and at the same time promote the male sexual function of rats, and has an application prospect of modifying and improving male reproductive function.

The invention relates to the cosmetic and non-therapeutic use of a composition consisting essentially of a compound of formula I below: (I) or its enantiomers, isomers or diastereoisomers, or a mixture thereof, or a salt or a solvate thereof, wherein R1 and R2, independently of each other, are a C3-C6 sugar, a uronic acid, or a glucoside, for bleaching or whitening the skin and skin appendages of a healthy individual.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The invention discloses a pharmaceutical composition for inducing leukemia cell copper death and application thereof, and relates to the technical field of tumor cell apoptosis. The active ingredients of the pharmaceutical composition are oleanolic acid and illlisemom. It is found for the first time that oleanolic acid can significantly up-regulate expression of a copper death key regulatory factor FDX1, when oleanolic acid is used in combination with a copper ion carrier Ilismor, a large synergistic effect can be generated through an FDX1 / DLAT axis, leukemia cells are significantly induced to generate copper death, and therefore in-vitro proliferation and in-vivo tumor forming ability of the leukemia cells are effectively inhibited. The composition provides a new drug development strategy with a clear mechanism for treatment of leukemia, especially drug-resistant leukemia.

The application discloses a method for preparing oleanolic acid-beta-D-glucopyranosyl ester from makaline, and relates to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: (1) makaline is extracted by using a solvent to obtain an extraction solution, and then solvent evaporation treatment is performed to obtain a first extract; (2) the first extract is preliminarily separated by using a macroporous resin chromatography, elution is performed by using different gradient concentrations of ethanol solution, 20% (volume concentration) ethanol eluent is obtained, and then solvent evaporation treatment is performed to obtain a second extract; and (3) the second extract is separated by using a high-speed counter-current chromatography, a fraction corresponding to a first sample peak is collected, and then drying treatment is performed, so that the oleanolic acid-beta-D-glucopyranosyl ester is obtained. The method provides a new approach for the separation and preparation of the oleanolic acid-beta-D-glucopyranosyl ester, and the method is simple, fast and high in preparation purity.

The application discloses application of oleanolic acid in promoting colonization of probiotic EcN strain in a gastrointestinal tract, and the oleanolic acid can promote formation of a biofilm of the probiotic EcN, enhance hydrophobicity of a surface of the probiotic EcN strain, and inhibit movement ability of the EcN strain, thereby benefiting adhesion and aggregation of the EcN strain, and further stabilizing colonization and survival in the intestinal tract, and having potential to be developed into probiotic food and preparations.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an inhalable powder preparation based on screened lactose and a preparation method of the inhalable powder preparation. The inhalable powder preparation is prepared from the following raw materials in parts by weight: 1 to 1.25 parts of active pharmaceutical ingredients, 55 to 60 parts of screened lactose, 5 to 12 parts of modified lactose fine powder, 1 to 4 parts of hydroxypropyl-beta-cyclodextrin and 1 to 3 parts of oleanolic acid beta-D-glucopyranosyl ester. The modified lactose fine powder is palmitoyl chloride grafted and modified lactose fine powder, and the modification treatment can weaken the formation ability of a hydration layer on the surface of lactose, reduce the surface polarity and generate steric hindrance, so that the self-agglomeration tendency among the lactose fine powder is remarkably reduced, and the binding characteristic of the lactose fine powder and medicine particles is improved; furthermore, the dispersing performance and the lung deposition efficiency of the preparation are remarkably improved. In addition, the hydroxypropyl-beta-cyclodextrin and the oleanolic acid beta-D-glucopyranosyl ester are matched for use, so that the stability of the preparation can be remarkably improved.

This invention provides a method for preparing camellia oil rich in oleanolic acid, comprising the following steps: (1) washing and draining fresh camellia fruit shells and / or tea leaves, then pulverizing them after vacuum freeze-drying to obtain freeze-dried powder A, and pulverizing dried camellia seed kernels to obtain seed kernel powder B. (2) mixing freeze-dried powder A and seed kernel powder B at a mass ratio of 1:(15-20). (3) adding a 25%-35% ethanol aqueous solution as an extraction solvent to the mixed raw materials, wherein the mass-volume ratio (g:mL) of the mixed raw materials to the extraction solvent is 1:(3-4); using a high-speed shearing device to vigorously stir and crush the mixed system for 3-10 minutes to achieve full cell wall disruption of the plant cells. (4) heating and reflux extraction; (5) three-phase separation and solvent reuse. This method has a high efficiency in transferring oleanolic acid from the raw materials to the oil phase.

The application relates to the field of cosmetics, and relates to an anti-aging cosmetic, grape pomace oil and a preparation method thereof. The anti-aging cosmetic comprises grape pomace oil; characteristic components of the grape pomace oil include oleanolic acid, alpha-guaiacwoodene, beta-tocotrienol, beta-sitosterol, campesterol, soybean sapogenol E, ganoderic acid F, ganoderol I and D-erythro-dihydrosphingosine; the total phenol content of the grape pomace oil is greater than or equal to 1 mg / g, the total sterol content of the grape pomace oil is greater than or equal to 26 mg / g, and the total flavonoid content of the grape pomace oil is greater than or equal to 1 mg / g; the grape pomace oil is extracted from grape pomace; the grape pomace is mainly composed of grape skin, grape stem and grape seed. The compounds have excellent antioxidant properties and can improve the anti-aging effect of the anti-aging cosmetic. The compounds are helpful for protecting the skin from oxidative damage and play a positive role in cosmetics. The grape pomace oil maximally retains active components of all parts of the grape pomace.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The present invention provides an animal feed additive with anti-inflammatory and antioxidant functions, namely, a traditional Chinese medicine-type feed additive with anti-biotic efficacy. The preparation method comprises the following steps: mixing a dried olive pomace powder sample with a subcritical extractant, performing subcritical extraction, and obtaining an extraction system; removing the subcritical extractant from the extraction system to obtain a pomace powder system from which pomace oil has been removed; mixing the pomace powder system with ethanol, performing leaching, and subjecting the obtained extract to vacuum distillation to obtain an extract; mixing the extract system with petroleum ether, allowing the petroleum ether phase to stand for stratification, and filtering the obtained petroleum ether phase to obtain an extract. The maslinic acid and oleanolic acid extracted by the present invention have anti-inflammatory and antioxidant effects, and animal experiments have confirmed that they can enhance intestinal health in a short period of time. The addition of a polypeptide improves the side effects of the maslinic acid and oleanolic acid caused by their long-term use. The present invention can be promoted and used as a new animal feed additive.

The invention discloses a pH-responsive HG type pectin hydrogel based on Schiff base reaction and a preparation method and application thereof, the pH-responsive HG type pectin hydrogel has a structural formula as shown in the following formula I. According to the pH-responsive HG type pectin hydrogel, functional characteristics of HG type pectin, biological activity of oleanolic acid, 3, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3- The intelligent hydrogel system with ROS / pH dual response characteristics is successfully constructed through the redox responsiveness of oleanolic acid, 3, 3 '-dithiodipropionic acid and the pH responsiveness of Schiff base reaction, and the system can effectively protect the activity of oleanolic acid and can be used for preparing drugs for improving alcoholic liver injury.

The present application relates to the technical field of multilayer liposome, and particularly relates to a polysaccharide double-layer modified liposome and preparation and application thereof. The polysaccharide double-layer modified liposome takes the oleanolic acid (OA) liposome as a core, and is coated with chitosan (CHI) and polygalacturonic acid (PGA) two kinds of polysaccharides on the surface in sequence to form a double-layer modified oleanolic acid liposome (PGA-CHI-OA liposome) with a core-shell structure. The modifiers chitosan and polygalacturonic acid used are food raw materials, and are low in cost and rich in resources. Under certain conditions, the two can be self-assembled on the surface of the liposome by mutual attraction of positive and negative charges, the preparation method is simple, the PGA-CHI-OA liposome particles formed are uniform in dispersion, and the PGA-CHI-OA liposome particles are high in safety, stability and bioavailability, and can be applied to food and health products.

In the field of biopharmaceutical analytical instruments, we provide a multi-temperature gradient heating device for use in oleanolic acid cell thermal shift assays. [Solution] A partition frame is provided on a base 1 to form multiple independent heating compartments, and an independently controllable heating block is placed in each compartment. An insulating sheet is provided between adjacent compartments to block heat exchange, and a low thermal conductivity positioning cover 5 with through holes for positioning reaction tubes is placed over the top. As a result, multiple temperature gradient conditions can be synchronized and stably heated in a single experimental operation through physical separation and independent heating, improving experimental efficiency and data comparability in the evaluation of oleanolic acid binding. Conventionally, in cell thermal shift assays, when evaluating the binding of oleanolic acid to target proteins, there was no means to synchronize and stably heat multiple temperature gradient conditions in a single operation, which presented challenges in experimental efficiency and data consistency.

The invention discloses a pentacyclic triterpene acylhydrazone compound as well as a preparation method and application thereof. A series of oleanolic acid derivatives are synthesized by carrying out structural modification on carboxyl at C28 site of oleanolic acid, structural confirmation is carried out by using 1H NMR, 13C NMR, HRMS and X-ray single crystal diffraction, and the inhibition effect of the pentacyclic triterpenoid acylhydrazone compound on the activity of tumor cells A549, AGS and K562 is evaluated by taking cis-platinum as positive control and adopting a CCK-8 method. Results show that the inhibition effect of the derivatives 5, 6, 9, 10, 16, 21, 27 and 28 on tumor cells is superior to that of cis-platinum. Reference is provided for research of oleanolic acid derivatives and discovery of anti-tumor seedling compounds.

The application relates to the technical field of organic synthesis and analytical chemistry, and particularly discloses a fluorescent probe based on a triphenyl oxazole derivative, which is chemically named as 2,2'-((1Z,1'Z)-((S)-[1,1'-binaphthalene]-2,2'-diyl)bis(azomethine))bis(4-(4,5-diphenyloxazole-2-yl)phenol), has a molecular formula of C 64 H 42 N4O4, and the fluorescent probe can be used for sensitively and selectively detecting oleanolic acid, and the fluorescence intensity is obviously enhanced during the detection of oleanolic acid, and the fluorescence wavelength is accompanied by a blue shift, so the fluorescent probe has a good application prospect.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition containing an effective component of American ginseng, a pharmaceutical preparation and application of the pharmaceutical composition and the pharmaceutical preparation in treating chronic obstructive pulmonary diseases. The pharmaceutical composition is prepared from the following components in parts by weight: 1 to 2 parts of ginsenoside Rg1, 1 to 2 parts of ginsenoside Rc, 0.5 to 1 part of oleanolic acid, 0.1 to 0.5 part of notoginsenoside R1 and 0.05 to 0.1 part of notoginsenoside Fe. Specific American ginseng saponin components are compounded, and the dosage of each component is optimized, so that the composition has remarkable effects of protecting the lung function of a COPD mouse caused by cigarette smoke exposure, inhibiting expression of inflammatory factors and relieving in-vivo oxidative stress injury, the repairing effect is remarkable, and in order to improve the effect of American ginseng in treatment of COPD, the composition has a good application prospect. And an important basis is provided for fully exerting the effects of all active components in the American ginseng.

The present invention discloses an oleanane-type triazole glycoside compound and its application in the preparation of PTP1B inhibitors and anti-diabetic drugs. The structural formula of the compound is: wherein R represents a saccharide group, which is oleanolic acid C 28 The oleanane-type triazole glycoside is prepared by converting the 3-hydroxyl group of methyl n-aminohexanoate into a 4-pentynyl ester as the starting material, followed by a click reaction with an azidosugar compound to introduce different sugar units. Pharmacological activity tests have shown that the oleanane-type triazole glycoside has excellent inhibitory activity against protein tyrosine phosphatase 1B and can be studied and utilized as a novel PTP1B inhibitor and antidiabetic drug.

The application belongs to the technical field of medicine, and discloses a pentacyclic triterpene derivative, a preparation method and medical application of the pentacyclic triterpene derivative, which uses alpha-hederin or oleanolic acid as raw material, and utilizes esterification reaction or amidation reaction aiming at the carboxyl of C-28 to prepare the pentacyclic triterpene derivative. The pentacyclic triterpene derivative prepared by the application has the effects of anti-inflammation, anti-oxidation and anti-apoptosis, and the synthesis method is simple, the raw material is cheap and easy to obtain, and is suitable for industrial application.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

Disclosed herein are C4 modified oleanolic acid derivatives of the formula:as well as analogs thereof, wherein the variables are defined herein. In addition, disclosed herein are pharmaceutical compositions of these derivatives or analogs, methods for their manufacture, and methods for their use, including for the prevention and treatment of diseases or disorders associated with overproduction of IL-17.

The invention belongs to the technical field of medicines, and particularly relates to an application of oleanolic acid in tumor resistance, and the application of oleanolic acid in tumor resistance specifically comprises the following steps: inhibiting tumor cell proliferation and inducing apoptosis; tumor angiogenesis is inhibited; the drug resistance of tumor cells is reversed; the immune function is regulated, and the anti-tumor immunity is enhanced; tumor invasion and metastasis are inhibited; according to the method, supercritical CO2 extraction is adopted, recoverable CO2 is used as an extraction medium, and no toxic organic solvent such as chloroform is used in the whole process; a small amount of ethanol is used only in the links of resin activation and elution, and is recovered through vacuum concentration, so that the use amount is extremely small, the treatment is easy, and the environmental pollution caused by solvent leakage or residue is avoided from the source.

Oral care films and oral care compositions containing such oral care films are disclosed herein. The oral care films may comprise from about 5 to about 19 wt.%, based on the total weight of the oral care film, of oleanolic acid and / or a salt thereof; a hydroxide; a polyol; a non-ionic surfactant; and a starch.

The invention discloses a composition for improving bioavailability of an oleanolic acid active component and a preparation method of the composition. The preparation method comprises the following steps: mixing proline pro and glucose Glu, heating and stirring in a water bath at 60 DEG C to prepare a natural eutectic solvent, mixing the natural eutectic solvent with oleanolic acid OA, stirring in a water bath at 80 DEG C, and homogenizing to obtain the composition capable of improving the bioavailability of oleanolic acid. When the molar ratio of the proline pro to the glucose Glu is 1: 1, the bioavailability of the prepared composition to the OA in a cell experiment can reach 19.01%, and meanwhile, the total absorption rate of cells to the OA can reach 91.73%-95.08%. Meanwhile, the composition provided by the invention has antioxidant activity, can effectively weaken oxidative damage in cells, and has application prospects in biological utilization of oleanolic acid and improvement of oxidation resistance of organisms.