81 results about "Oleanonic acid" patented technology

An aqueous oleanolic acid solution, including from about 0.10 to 2.5 weight % oleanolic acid, from about 60 to 90 weight % solvent, and from about 20 to 40 weight % solubility agent, wherein the solvent includes water, the solubility agent includes sodium lauryl sulfate (SLS), and the aqueous oleanolic acid solution is substantially free of ethanol and dimethyl sulfoxide (DMSO).

A use of oleanolic acid-28-O-β-D-glucopyranoside in the preparation of an anti-colitis drug is provided. The oleanolic acid-28-O-β-D-glucopyranoside has significant anti-ulcerative-colitis activity compared with parent nucleus oleanolic acid and other analogues, and has equivalent efficacy compared with Mesalazine, a first-line drug for the clinical treatment of inflammatory bowel disease, and can also avoid salicylic acid anaphylaxis possibly caused by Mesalazine western medicine treatment, thereby achieving a good application prospect.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of oleanolic acid in promoting chondrogenic differentiation of chondrocytes. This invention discovers that oleanolic acid promotes the expression of proteoglycans and calcium deposition in ATDC5 cells undergoing chondrogenic differentiation, and also promotes the expression of ATDC5 cell chondrogenic differentiation markers. Therefore, it has the potential to be formulated into a drug that can promote chondrogenic differentiation of chondrocytes, thereby promoting cartilage repair and regeneration for the treatment of osteoarthritis and rheumatoid arthritis.

The invention relates to the cosmetic and non-therapeutic use of a composition consisting essentially of a compound of formula I below: (I) or its enantiomers, isomers or diastereoisomers, or a mixture thereof, or a salt or a solvate thereof, wherein R1 and R2, independently of each other, are a C3-C6 sugar, a uronic acid, or a glucoside, for bleaching or whitening the skin and skin appendages of a healthy individual.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The invention discloses a pharmaceutical composition for inducing leukemia cell copper death and application thereof, and relates to the technical field of tumor cell apoptosis. The active ingredients of the pharmaceutical composition are oleanolic acid and illlisemom. It is found for the first time that oleanolic acid can significantly up-regulate expression of a copper death key regulatory factor FDX1, when oleanolic acid is used in combination with a copper ion carrier Ilismor, a large synergistic effect can be generated through an FDX1 / DLAT axis, leukemia cells are significantly induced to generate copper death, and therefore in-vitro proliferation and in-vivo tumor forming ability of the leukemia cells are effectively inhibited. The composition provides a new drug development strategy with a clear mechanism for treatment of leukemia, especially drug-resistant leukemia.

The application discloses a method for preparing oleanolic acid-beta-D-glucopyranosyl ester from makaline, and relates to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: (1) makaline is extracted by using a solvent to obtain an extraction solution, and then solvent evaporation treatment is performed to obtain a first extract; (2) the first extract is preliminarily separated by using a macroporous resin chromatography, elution is performed by using different gradient concentrations of ethanol solution, 20% (volume concentration) ethanol eluent is obtained, and then solvent evaporation treatment is performed to obtain a second extract; and (3) the second extract is separated by using a high-speed counter-current chromatography, a fraction corresponding to a first sample peak is collected, and then drying treatment is performed, so that the oleanolic acid-beta-D-glucopyranosyl ester is obtained. The method provides a new approach for the separation and preparation of the oleanolic acid-beta-D-glucopyranosyl ester, and the method is simple, fast and high in preparation purity.

The application discloses application of oleanolic acid in promoting colonization of probiotic EcN strain in a gastrointestinal tract, and the oleanolic acid can promote formation of a biofilm of the probiotic EcN, enhance hydrophobicity of a surface of the probiotic EcN strain, and inhibit movement ability of the EcN strain, thereby benefiting adhesion and aggregation of the EcN strain, and further stabilizing colonization and survival in the intestinal tract, and having potential to be developed into probiotic food and preparations.

This invention provides a method for preparing camellia oil rich in oleanolic acid, comprising the following steps: (1) washing and draining fresh camellia fruit shells and / or tea leaves, then pulverizing them after vacuum freeze-drying to obtain freeze-dried powder A, and pulverizing dried camellia seed kernels to obtain seed kernel powder B. (2) mixing freeze-dried powder A and seed kernel powder B at a mass ratio of 1:(15-20). (3) adding a 25%-35% ethanol aqueous solution as an extraction solvent to the mixed raw materials, wherein the mass-volume ratio (g:mL) of the mixed raw materials to the extraction solvent is 1:(3-4); using a high-speed shearing device to vigorously stir and crush the mixed system for 3-10 minutes to achieve full cell wall disruption of the plant cells. (4) heating and reflux extraction; (5) three-phase separation and solvent reuse. This method has a high efficiency in transferring oleanolic acid from the raw materials to the oil phase.

The application relates to the field of cosmetics, and relates to an anti-aging cosmetic, grape pomace oil and a preparation method thereof. The anti-aging cosmetic comprises grape pomace oil; characteristic components of the grape pomace oil include oleanolic acid, alpha-guaiacwoodene, beta-tocotrienol, beta-sitosterol, campesterol, soybean sapogenol E, ganoderic acid F, ganoderol I and D-erythro-dihydrosphingosine; the total phenol content of the grape pomace oil is greater than or equal to 1 mg / g, the total sterol content of the grape pomace oil is greater than or equal to 26 mg / g, and the total flavonoid content of the grape pomace oil is greater than or equal to 1 mg / g; the grape pomace oil is extracted from grape pomace; the grape pomace is mainly composed of grape skin, grape stem and grape seed. The compounds have excellent antioxidant properties and can improve the anti-aging effect of the anti-aging cosmetic. The compounds are helpful for protecting the skin from oxidative damage and play a positive role in cosmetics. The grape pomace oil maximally retains active components of all parts of the grape pomace.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The invention discloses a pH-responsive HG type pectin hydrogel based on Schiff base reaction and a preparation method and application thereof, the pH-responsive HG type pectin hydrogel has a structural formula as shown in the following formula I. According to the pH-responsive HG type pectin hydrogel, functional characteristics of HG type pectin, biological activity of oleanolic acid, 3, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3- The intelligent hydrogel system with ROS / pH dual response characteristics is successfully constructed through the redox responsiveness of oleanolic acid, 3, 3 '-dithiodipropionic acid and the pH responsiveness of Schiff base reaction, and the system can effectively protect the activity of oleanolic acid and can be used for preparing drugs for improving alcoholic liver injury.

The present application relates to the technical field of multilayer liposome, and particularly relates to a polysaccharide double-layer modified liposome and preparation and application thereof. The polysaccharide double-layer modified liposome takes the oleanolic acid (OA) liposome as a core, and is coated with chitosan (CHI) and polygalacturonic acid (PGA) two kinds of polysaccharides on the surface in sequence to form a double-layer modified oleanolic acid liposome (PGA-CHI-OA liposome) with a core-shell structure. The modifiers chitosan and polygalacturonic acid used are food raw materials, and are low in cost and rich in resources. Under certain conditions, the two can be self-assembled on the surface of the liposome by mutual attraction of positive and negative charges, the preparation method is simple, the PGA-CHI-OA liposome particles formed are uniform in dispersion, and the PGA-CHI-OA liposome particles are high in safety, stability and bioavailability, and can be applied to food and health products.

In the field of biopharmaceutical analytical instruments, we provide a multi-temperature gradient heating device for use in oleanolic acid cell thermal shift assays. [Solution] A partition frame is provided on a base 1 to form multiple independent heating compartments, and an independently controllable heating block is placed in each compartment. An insulating sheet is provided between adjacent compartments to block heat exchange, and a low thermal conductivity positioning cover 5 with through holes for positioning reaction tubes is placed over the top. As a result, multiple temperature gradient conditions can be synchronized and stably heated in a single experimental operation through physical separation and independent heating, improving experimental efficiency and data comparability in the evaluation of oleanolic acid binding. Conventionally, in cell thermal shift assays, when evaluating the binding of oleanolic acid to target proteins, there was no means to synchronize and stably heat multiple temperature gradient conditions in a single operation, which presented challenges in experimental efficiency and data consistency.

The invention discloses a pentacyclic triterpene acylhydrazone compound as well as a preparation method and application thereof. A series of oleanolic acid derivatives are synthesized by carrying out structural modification on carboxyl at C28 site of oleanolic acid, structural confirmation is carried out by using 1H NMR, 13C NMR, HRMS and X-ray single crystal diffraction, and the inhibition effect of the pentacyclic triterpenoid acylhydrazone compound on the activity of tumor cells A549, AGS and K562 is evaluated by taking cis-platinum as positive control and adopting a CCK-8 method. Results show that the inhibition effect of the derivatives 5, 6, 9, 10, 16, 21, 27 and 28 on tumor cells is superior to that of cis-platinum. Reference is provided for research of oleanolic acid derivatives and discovery of anti-tumor seedling compounds.

The application relates to the technical field of organic synthesis and analytical chemistry, and particularly discloses a fluorescent probe based on a triphenyl oxazole derivative, which is chemically named as 2,2'-((1Z,1'Z)-((S)-[1,1'-binaphthalene]-2,2'-diyl)bis(azomethine))bis(4-(4,5-diphenyloxazole-2-yl)phenol), has a molecular formula of C 64 H 42 N4O4, and the fluorescent probe can be used for sensitively and selectively detecting oleanolic acid, and the fluorescence intensity is obviously enhanced during the detection of oleanolic acid, and the fluorescence wavelength is accompanied by a blue shift, so the fluorescent probe has a good application prospect.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition containing an effective component of American ginseng, a pharmaceutical preparation and application of the pharmaceutical composition and the pharmaceutical preparation in treating chronic obstructive pulmonary diseases. The pharmaceutical composition is prepared from the following components in parts by weight: 1 to 2 parts of ginsenoside Rg1, 1 to 2 parts of ginsenoside Rc, 0.5 to 1 part of oleanolic acid, 0.1 to 0.5 part of notoginsenoside R1 and 0.05 to 0.1 part of notoginsenoside Fe. Specific American ginseng saponin components are compounded, and the dosage of each component is optimized, so that the composition has remarkable effects of protecting the lung function of a COPD mouse caused by cigarette smoke exposure, inhibiting expression of inflammatory factors and relieving in-vivo oxidative stress injury, the repairing effect is remarkable, and in order to improve the effect of American ginseng in treatment of COPD, the composition has a good application prospect. And an important basis is provided for fully exerting the effects of all active components in the American ginseng.

The application belongs to the technical field of medicine, and discloses a pentacyclic triterpene derivative, a preparation method and medical application of the pentacyclic triterpene derivative, which uses alpha-hederin or oleanolic acid as raw material, and utilizes esterification reaction or amidation reaction aiming at the carboxyl of C-28 to prepare the pentacyclic triterpene derivative. The pentacyclic triterpene derivative prepared by the application has the effects of anti-inflammation, anti-oxidation and anti-apoptosis, and the synthesis method is simple, the raw material is cheap and easy to obtain, and is suitable for industrial application.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

Disclosed herein are C4 modified oleanolic acid derivatives of the formula:as well as analogs thereof, wherein the variables are defined herein. In addition, disclosed herein are pharmaceutical compositions of these derivatives or analogs, methods for their manufacture, and methods for their use, including for the prevention and treatment of diseases or disorders associated with overproduction of IL-17.

The invention belongs to the technical field of medicines, and particularly relates to an application of oleanolic acid in tumor resistance, and the application of oleanolic acid in tumor resistance specifically comprises the following steps: inhibiting tumor cell proliferation and inducing apoptosis; tumor angiogenesis is inhibited; the drug resistance of tumor cells is reversed; the immune function is regulated, and the anti-tumor immunity is enhanced; tumor invasion and metastasis are inhibited; according to the method, supercritical CO2 extraction is adopted, recoverable CO2 is used as an extraction medium, and no toxic organic solvent such as chloroform is used in the whole process; a small amount of ethanol is used only in the links of resin activation and elution, and is recovered through vacuum concentration, so that the use amount is extremely small, the treatment is easy, and the environmental pollution caused by solvent leakage or residue is avoided from the source.

The invention discloses a composition for improving bioavailability of an oleanolic acid active component and a preparation method of the composition. The preparation method comprises the following steps: mixing proline pro and glucose Glu, heating and stirring in a water bath at 60 DEG C to prepare a natural eutectic solvent, mixing the natural eutectic solvent with oleanolic acid OA, stirring in a water bath at 80 DEG C, and homogenizing to obtain the composition capable of improving the bioavailability of oleanolic acid. When the molar ratio of the proline pro to the glucose Glu is 1: 1, the bioavailability of the prepared composition to the OA in a cell experiment can reach 19.01%, and meanwhile, the total absorption rate of cells to the OA can reach 91.73%-95.08%. Meanwhile, the composition provided by the invention has antioxidant activity, can effectively weaken oxidative damage in cells, and has application prospects in biological utilization of oleanolic acid and improvement of oxidation resistance of organisms.

The invention discloses an aquatic product liver protection feed additive based on a multi-target synergistic effect as well as a preparation method and application thereof, and belongs to the technical field of aquatic product feed additives. According to the invention, betaine hydrochloride, bile acid, taurine, macleaya cordata extract, ligustrum lucidum polysaccharide, oleanolic acid, glucurolactone, salidroside, vitamin E and nano-selenium are used as raw materials to prepare the aquatic product feed additive capable of protecting the liver, and the ligustrum lucidum polysaccharide and the salidroside play a role in protecting the liver; bile acid, taurine and betaine hydrochloride are used for promoting fat metabolism, vitamin E and nano-selenium play an anti-oxidation role, glucurolactone plays a detoxification role, oleanolic acid and macleaya cordata extract play an anti-inflammatory role, and vitamin E and nano-selenium play an anti-inflammatory role. The prepared liver-protecting feed additive for the aquatic products can cope with abnormal conditions such as hepatomegaly, white liver and green liver caused by excessive feeding, metamorphic feed feeding, water quality deterioration, pathogenic bacteria infection and the like.

The invention relates to the technical field of preparation of olive leaf extracts, in particular to an olive leaf extract and a preparation process and application thereof, and the process comprises the following steps: crushing dried olive leaves, mixing the crushed olive leaves with an ethanol aqueous solution, and carrying out multi-stage temperature control hot reflux extraction and solid-liquid separation to obtain a supernatant and a precipitate; loading the supernate to a resin column, washing with water and then eluting with ethanol, and drying the eluent to obtain an oleuropein crude product; blending the oleuropein crude product and the sample stirring silica gel, drying, filling into a chromatographic column, eluting by using an ethyl acetate-ethanol mixed solvent, and collecting a high-purity oleuropein fine product; sequentially carrying out ultrasonic dissolution, concentration, standing crystallization and filtration on the precipitate through medium-concentration and high-concentration ethanol to obtain a maslinic acid crude product and an oleanolic acid crude product; and respectively heating and dissolving with ethanol, cooling and crystallizing, and circularly recrystallizing to obtain a corresponding refined product. The invention aims to guarantee the extraction yield and quality of oleuropein by controlling the multi-stage temperature of heating reflux extraction of olive leaves.

The invention relates to the technical field of materials, and discloses a medical gel dressing for wound repair and a preparation process thereof.The medical gel dressing is prepared by taking aldehyde konjac glucomannan and aldehyde sodium hyaluronate as base materials and mixing the base materials with a cross-linking agent ethyl deionized water, wherein due to the existence of the cross-linking agent, all the raw materials in the gel dressing can become an interconnected whole, so that the gel has higher stability and mechanical performance, meanwhile, the liquid absorption performance of the gel dressing is improved, in addition, the oleanolic acid of the cross-linking agent structure can endow the gel dressing with excellent antibacterial performance, and the gel dressing has good application prospects. Ether bonds in a cross-linking agent structure can enhance the water absorption of the gel dressing and improve the absorption effect of the gel dressing on wound exudate, in addition, aldehyde sodium hyaluronate is used as a base material of the gel dressing and can cooperate with oleanolic acid, the antibacterial performance of the gel dressing is greatly improved, wound infection caused by bacterial erosion is prevented, and the gel dressing has a good application prospect. The wound healing and repairing speed is influenced.

The invention relates to the technical field of biological materials, in particular to lipid nanoparticles based on modified natural saponin, a preparation method and application of the lipid nanoparticles, and the modified natural saponin comprises natural saponin modified by metal complexes and / or natural saponin modified by targeting molecules. The natural saponin is selected from at least one of glycyrrhizic acid, aescin, soyasaponin, ginsenoside, ursolic acid and oleanolic acid. The modified natural saponin can effectively simulate the structural function of cholesterol compounds in lipid nanoparticles, can overcome the problems of unstable physicochemical properties of cholesterol, potential safety hazards of sources and the like, and can significantly improve the gene transfection and silencing efficiency of nucleic acid drugs as a drug carrier. And a new solution is provided for gene therapy and vaccine development. The preparation method of the lipid nanoparticles based on the modified natural saponin is mild in reaction condition, simple and convenient to operate, easy to control and beneficial to industrial production.

The application belongs to the technical field of pharmaceutical preparations, and particularly relates to an inhalable powder preparation based on sieved lactose and a preparation method thereof. The inhalable powder preparation comprises the following raw materials in parts by weight: 1-1.25 parts of a pharmaceutical active ingredient, 55-60 parts of sieved lactose, 5-12 parts of modified lactose fine powder, 1-4 parts of hydroxypropyl-beta-cyclodextrin, and 1-3 parts of oleanolic acid beta-D-glucopyranosyl ester. The modified lactose fine powder is lactose fine powder grafted with palmitoyl chloride. The modification can weaken the formation ability of the lactose surface hydration layer, reduce the surface polarity, and produce steric hindrance, thereby significantly reducing the self-agglomeration tendency between the fine powder lactose, improving the combination characteristics of the fine powder lactose and the pharmaceutical particles, and further significantly improving the dispersion performance and lung deposition efficiency of the preparation. In addition, the combination of hydroxypropyl-beta-cyclodextrin and oleanolic acid beta-D-glucopyranosyl ester can significantly improve the stability of the preparation.

The invention relates to an antibacterial and deodorant finishing agent for down feather and a preparation method thereof, and belongs to the technical field of down feather processing.The preparation method comprises the steps that red thick-shell branches and leaves are sequentially cleaned, baked and smashed, a fermentation raw material is obtained, dendrobe smashed materials, phytase and water are evenly mixed, a culture medium is obtained, bifidobacterium is inoculated into the culture medium, and the antibacterial and deodorant finishing agent for down feather is obtained. The preparation method comprises the following steps: uniformly mixing raw materials, culturing to obtain a fermentation substrate, uniformly mixing the fermentation raw materials into the fermentation substrate, standing for fermentation, carrying out centrifugal filtration treatment on a fermentation product to obtain red thick-shell fermentation liquor, and uniformly mixing the red thick-shell fermentation liquor, oleanolic acid and a cross-linking agent to obtain the finishing agent. The invention provides a finishing agent containing red thick-shell fermentation liquor, oleanolic acid and water, and further provides a red thick-shell fermentation system containing crushed dendrobe, phytase and bifidobacterium, so that the effects of endowing the down feather with good antibacterial property and odor adhesion prevention are comprehensively realized.

The invention provides a composition comprising oleanolic acid and the salts, prodrug and solvates thereof; and one or more compounds selected from the following group: ellagic acid, adenine, betaine, choline, miquelianin, p-coumaric acid, pipecolic acid, protocatechuic acid and tyramine, and the salts, prodrugs and solvates thereof. Such a composition is for use in reducing lipid levels in sebocytes, and the composition may find use in treating acne vulgaris and rosacea.

The invention discloses a preparation method of a natural pentacyclic triterpenoid-curcumin self-assembly system with a synergistic anti-oxidation function, and belongs to the field of functional foods. The method comprises the following steps: completely dissolving oleanolic acid and curcumin in a self-assembly solvent to obtain a mixed solution; adding the mixed solution into an amphiphilic aqueous solution drop by drop under vortex oscillation, and carrying out ultrasonic treatment (ultrasonic treatment for 5 seconds and interval for 5 seconds) for 60 seconds at the ultrasonic power of 200-800 W to obtain an oleanolic acid-curcumin self-assembled nanoparticle (OA-CUR SNPs) pre-assembled solution; transferring the OA-CUR SNPs pre-assembled solution into ultrapure water, carrying out microwave treatment for 5-45 minutes at the power of 100-600 W, and then carrying out self-assembly for 1-6 hours under magnetic stirring at the speed of 200-600 r / min; dialyzing for 12-24 hours in a dialysis bag with the molecular weight cut-off of 5000Da, carrying out reduced-pressure rotary evaporation to recover a sample, pre-freezing the sample at the temperature of 80 DEG C below zero for at least 2 hours, and freeze-drying. According to the invention, the self-assembly effect between oleanolic acid and curcumin molecules can be utilized, and a self-assembly nano system with high load, good stability and enhanced synergistic antioxidant activity is constructed by a one-step method.