40 results about "Oleanonic acid" patented technology

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of oleanolic acid in promoting chondrogenic differentiation of chondrocytes. This invention discovers that oleanolic acid promotes the expression of proteoglycans and calcium deposition in ATDC5 cells undergoing chondrogenic differentiation, and also promotes the expression of ATDC5 cell chondrogenic differentiation markers. Therefore, it has the potential to be formulated into a drug that can promote chondrogenic differentiation of chondrocytes, thereby promoting cartilage repair and regeneration for the treatment of osteoarthritis and rheumatoid arthritis.

The invention discloses a pharmaceutical composition for inducing leukemia cell copper death and application thereof, and relates to the technical field of tumor cell apoptosis. The active ingredients of the pharmaceutical composition are oleanolic acid and illlisemom. It is found for the first time that oleanolic acid can significantly up-regulate expression of a copper death key regulatory factor FDX1, when oleanolic acid is used in combination with a copper ion carrier Ilismor, a large synergistic effect can be generated through an FDX1 / DLAT axis, leukemia cells are significantly induced to generate copper death, and therefore in-vitro proliferation and in-vivo tumor forming ability of the leukemia cells are effectively inhibited. The composition provides a new drug development strategy with a clear mechanism for treatment of leukemia, especially drug-resistant leukemia.

This invention provides a method for preparing camellia oil rich in oleanolic acid, comprising the following steps: (1) washing and draining fresh camellia fruit shells and / or tea leaves, then pulverizing them after vacuum freeze-drying to obtain freeze-dried powder A, and pulverizing dried camellia seed kernels to obtain seed kernel powder B. (2) mixing freeze-dried powder A and seed kernel powder B at a mass ratio of 1:(15-20). (3) adding a 25%-35% ethanol aqueous solution as an extraction solvent to the mixed raw materials, wherein the mass-volume ratio (g:mL) of the mixed raw materials to the extraction solvent is 1:(3-4); using a high-speed shearing device to vigorously stir and crush the mixed system for 3-10 minutes to achieve full cell wall disruption of the plant cells. (4) heating and reflux extraction; (5) three-phase separation and solvent reuse. This method has a high efficiency in transferring oleanolic acid from the raw materials to the oil phase.

The invention discloses a pH-responsive HG type pectin hydrogel based on Schiff base reaction and a preparation method and application thereof, the pH-responsive HG type pectin hydrogel has a structural formula as shown in the following formula I. According to the pH-responsive HG type pectin hydrogel, functional characteristics of HG type pectin, biological activity of oleanolic acid, 3, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3- The intelligent hydrogel system with ROS / pH dual response characteristics is successfully constructed through the redox responsiveness of oleanolic acid, 3, 3 '-dithiodipropionic acid and the pH responsiveness of Schiff base reaction, and the system can effectively protect the activity of oleanolic acid and can be used for preparing drugs for improving alcoholic liver injury.

The present application relates to the technical field of multilayer liposome, and particularly relates to a polysaccharide double-layer modified liposome and preparation and application thereof. The polysaccharide double-layer modified liposome takes the oleanolic acid (OA) liposome as a core, and is coated with chitosan (CHI) and polygalacturonic acid (PGA) two kinds of polysaccharides on the surface in sequence to form a double-layer modified oleanolic acid liposome (PGA-CHI-OA liposome) with a core-shell structure. The modifiers chitosan and polygalacturonic acid used are food raw materials, and are low in cost and rich in resources. Under certain conditions, the two can be self-assembled on the surface of the liposome by mutual attraction of positive and negative charges, the preparation method is simple, the PGA-CHI-OA liposome particles formed are uniform in dispersion, and the PGA-CHI-OA liposome particles are high in safety, stability and bioavailability, and can be applied to food and health products.

In the field of biopharmaceutical analytical instruments, we provide a multi-temperature gradient heating device for use in oleanolic acid cell thermal shift assays. [Solution] A partition frame is provided on a base 1 to form multiple independent heating compartments, and an independently controllable heating block is placed in each compartment. An insulating sheet is provided between adjacent compartments to block heat exchange, and a low thermal conductivity positioning cover 5 with through holes for positioning reaction tubes is placed over the top. As a result, multiple temperature gradient conditions can be synchronized and stably heated in a single experimental operation through physical separation and independent heating, improving experimental efficiency and data comparability in the evaluation of oleanolic acid binding. Conventionally, in cell thermal shift assays, when evaluating the binding of oleanolic acid to target proteins, there was no means to synchronize and stably heat multiple temperature gradient conditions in a single operation, which presented challenges in experimental efficiency and data consistency.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition containing an effective component of American ginseng, a pharmaceutical preparation and application of the pharmaceutical composition and the pharmaceutical preparation in treating chronic obstructive pulmonary diseases. The pharmaceutical composition is prepared from the following components in parts by weight: 1 to 2 parts of ginsenoside Rg1, 1 to 2 parts of ginsenoside Rc, 0.5 to 1 part of oleanolic acid, 0.1 to 0.5 part of notoginsenoside R1 and 0.05 to 0.1 part of notoginsenoside Fe. Specific American ginseng saponin components are compounded, and the dosage of each component is optimized, so that the composition has remarkable effects of protecting the lung function of a COPD mouse caused by cigarette smoke exposure, inhibiting expression of inflammatory factors and relieving in-vivo oxidative stress injury, the repairing effect is remarkable, and in order to improve the effect of American ginseng in treatment of COPD, the composition has a good application prospect. And an important basis is provided for fully exerting the effects of all active components in the American ginseng.

The application belongs to the technical field of medicine, and discloses a pentacyclic triterpene derivative, a preparation method and medical application of the pentacyclic triterpene derivative, which uses alpha-hederin or oleanolic acid as raw material, and utilizes esterification reaction or amidation reaction aiming at the carboxyl of C-28 to prepare the pentacyclic triterpene derivative. The pentacyclic triterpene derivative prepared by the application has the effects of anti-inflammation, anti-oxidation and anti-apoptosis, and the synthesis method is simple, the raw material is cheap and easy to obtain, and is suitable for industrial application.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

Disclosed herein are C4 modified oleanolic acid derivatives of the formula:as well as analogs thereof, wherein the variables are defined herein. In addition, disclosed herein are pharmaceutical compositions of these derivatives or analogs, methods for their manufacture, and methods for their use, including for the prevention and treatment of diseases or disorders associated with overproduction of IL-17.

The invention relates to the technical field of preparation of olive leaf extracts, in particular to an olive leaf extract and a preparation process and application thereof, and the process comprises the following steps: crushing dried olive leaves, mixing the crushed olive leaves with an ethanol aqueous solution, and carrying out multi-stage temperature control hot reflux extraction and solid-liquid separation to obtain a supernatant and a precipitate; loading the supernate to a resin column, washing with water and then eluting with ethanol, and drying the eluent to obtain an oleuropein crude product; blending the oleuropein crude product and the sample stirring silica gel, drying, filling into a chromatographic column, eluting by using an ethyl acetate-ethanol mixed solvent, and collecting a high-purity oleuropein fine product; sequentially carrying out ultrasonic dissolution, concentration, standing crystallization and filtration on the precipitate through medium-concentration and high-concentration ethanol to obtain a maslinic acid crude product and an oleanolic acid crude product; and respectively heating and dissolving with ethanol, cooling and crystallizing, and circularly recrystallizing to obtain a corresponding refined product. The invention aims to guarantee the extraction yield and quality of oleuropein by controlling the multi-stage temperature of heating reflux extraction of olive leaves.

The invention discloses a preparation method of a natural pentacyclic triterpenoid-curcumin self-assembly system with a synergistic anti-oxidation function, and belongs to the field of functional foods. The method comprises the following steps: completely dissolving oleanolic acid and curcumin in a self-assembly solvent to obtain a mixed solution; adding the mixed solution into an amphiphilic aqueous solution drop by drop under vortex oscillation, and carrying out ultrasonic treatment (ultrasonic treatment for 5 seconds and interval for 5 seconds) for 60 seconds at the ultrasonic power of 200-800 W to obtain an oleanolic acid-curcumin self-assembled nanoparticle (OA-CUR SNPs) pre-assembled solution; transferring the OA-CUR SNPs pre-assembled solution into ultrapure water, carrying out microwave treatment for 5-45 minutes at the power of 100-600 W, and then carrying out self-assembly for 1-6 hours under magnetic stirring at the speed of 200-600 r / min; dialyzing for 12-24 hours in a dialysis bag with the molecular weight cut-off of 5000Da, carrying out reduced-pressure rotary evaporation to recover a sample, pre-freezing the sample at the temperature of 80 DEG C below zero for at least 2 hours, and freeze-drying. According to the invention, the self-assembly effect between oleanolic acid and curcumin molecules can be utilized, and a self-assembly nano system with high load, good stability and enhanced synergistic antioxidant activity is constructed by a one-step method.

Compositions and methods are described for improving brain health and cognitive function and for preventing and treating Alzheimer's disease and other age-related dementia diseases and conditions for intake as a dietary or nutritional supplement, for example, as a powdered drink mix. Compositions for providing an energy source to the brain during exercise are also described. The composition includes a source of choline, a source of magnesium, at least one taurine-containing composition, a source of ashwagandha root, and phosphatidylserine. The source of choline can be alpha-glycerylphosphorylcholine or citicoline and the source of magnesium can be magnesium-L-threonate or magnesium glycinate. The composition can further include at least one of oleic acid, oleanolic acid, ursolic acid, curcumin, piperine, galantamine, huperzine-A, a source of vitamin B5, L-tyrosine, and beta-hydroxybutyrate.

This invention discloses an injectable AuPd-Fe single-atom nanozyme composite drug-derived antibacterial hydrogel, its preparation method, and its applications, belonging to the field of biomedical nanomaterials technology. This invention uses an iron-based single-atom nanozyme with an irregular octahedral carbon framework structure as the core catalytic carrier, and in situ loads ultra-small AuPd alloy nanoparticles onto its surface to obtain the AuPd-Fe single-atom nanozyme. Then, using the AuPd-Fe single-atom nanozyme as the active center and oleanolic acid drug-derived hydrogel as the delivery carrier, the AuPd-Fe single-atom nanozyme is uniformly dispersed in the three-dimensional network of the OA hydrogel through physical embedding and hydrogen bonding, resulting in the injectable AuPd-Fe single-atom nanozyme composite drug-derived antibacterial hydrogel. The provided antibacterial hydrogel exhibits excellent ocular surface biocompatibility, no significant corneal toxicity, and no systemic organ damage. It also shows significant clinical application potential for blinding ocular infections such as drug-resistant bacterial keratitis and corneal ulcers.

The present application relates to a kind of hydraulic cylinder flame-retardant buffer polyurethane pipe material and its forming process, belong to polyurethane pipe material technical field, according to weight parts, the preparation raw material of the polyurethane sizing material includes 65-80 parts polyurethane particle, 3-6 parts trimethylolpropane, 1-3 parts pentaerythritol, 5-8 parts peroxide carbonate, 10-15 parts white carbon black, 5-6 parts antioxidant, 2-5 parts zinc stearate.Polyurethane pipe material is prepared by using multiple components to obtain flame-retardant particles, which can effectively play a flame-retardant role when polyurethane pipe material is used, wherein, lignin and oleanolic acid cooperate to produce synergistic effect, realize efficient flame-retardant effect, so that polyurethane pipe material has excellent self-extinguishing performance away from fire, polyurethane pipe material can be used in some locations with higher temperature or closer to the source of fire, increase the use range of polyurethane pipe material.

Oral care compositions and, preferably, oral care compositions providing a reduction in gum bleeding as well as methods for producing such oral care compositions are disclosed herein. In accordance with some aspects, provided is an oral care composition having a stannous source; a nitrate source; a phosphate source; a surfactant system comprising a sulfate based anionic surfactant; oleanolic acid; and eugenol.

The invention provides an exosome for promoting hair follicle cell growth and a preparation method and application thereof.The method comprises the steps that firstly, Triton X-100 with extremely low concentration is adopted, and reversible micropores are formed in the surface of a membrane on the premise that the overall structure of the exosome is stable; and then effectively introducing astragaloside and oleanolic acid into the exosome under the action of ultrasonic waves. Compared with a traditional electrotransfection method, the method has the advantages that the damage to the exosome membrane is remarkably reduced, and the integrity of membrane protein and natural functions is maintained. Meanwhile, the anti-apoptosis effect of astragaloside and the Wnt / beta-catenin activation effect of oleanolic acid cooperate with each other, hair follicle cell proliferation can be promoted more remarkably, the growth period of hair follicles can be prolonged more remarkably, and therefore a brand new technical path is provided for alopecia treatment and hair follicle regeneration.

1. Name of the product in this design: Packaging Box (Oleanolic Acid Tablets). 2. Purpose of this design: For packaging pharmaceuticals. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the key design points: unfolded diagram.

The invention relates to the cosmetic and non-therapeutic use of a composition consisting essentially of a compound of formula I below: (I) or its enantiomers, isomers or diastereoisomers, or a mixture thereof, or a salt or a solvate thereof, wherein R1 and R2, independently of each other, are a C3-C6 sugar, a uronic acid, or a glucoside, for bleaching or whitening the skin and skin appendages of a healthy individual.

The application provides a tumor inhibiting composition and a preparation method and application thereof, and belongs to the technical field of medicines. After mesoporous carbon nanotubes are oxidized and chlorinated, oleanolic acid dithiocarbamate conjugate and (2-hydroxypropyl)-beta-cyclodextrin are coupled, drugs are loaded, a protein liposome solution is added, ultrasonic treatment is carried out, centrifugal separation is carried out, unloaded drug-loaded carbon nanotubes are removed, supernatant is freeze-dried, and the tumor inhibiting composition is prepared. The tumor inhibiting composition greatly improves the drug loading capacity of the nanosystem, the synergistic effect of multiple drugs, the anti-tumor effect, realizes the effects of targeting, slow and controlled release of drugs, improves the drug utilization rate, reduces the intake amount of drugs, reduces side effects, and improves the compliance of patients.

The invention discloses a pharmaceutical composition for inhibiting expression of leukemia tumor stem cell markers and application of the pharmaceutical composition, and relates to the technical field of leukocyte tumor cell inhibition. The active ingredients of the pharmaceutical composition are oleanolic acid and illlisemom. The invention reveals that oleanolic acid inhibits expression of key markers Sox9, Nanog, CD133 and CD44 of leukemia tumor stem cells by targeting FDX1 / DLAT axis for the first time, and further inhibits drug resistance and recurrence of malignant tumors. When the compound is used in combination with a copper ion carrier ilismor, a relatively large synergistic effect can be generated by inhibiting the expression of tumor stem cell markers Sox9, Nanog, CD133 and CD44, and the stemness of leukemia is remarkably inhibited. The composition provides a new drug development strategy with a clear mechanism for treatment of leukemia.

The application belongs to the technical field of biological medicine, and particularly relates to a C-3 substituted oleanolic acid benzamide derivative, a preparation method and application thereof. The C-3 substituted oleanolic acid benzamide derivative has a novel chemical structure, and has good inhibitory activity on influenza virus, especially on H1N1 influenza virus A in vitro and in vivo, which indicates that the compound can be prepared into an anti-influenza virus preparation, has a good application prospect in the prevention or / and treatment of influenza virus infection, and expands the application value of the C-3 substituted oleanolic acid benzamide derivative.

The invention relates to the technical field of biology, in particular to oleanolic acid glycosyl transferase, a recombinant strain and application of the oleanolic acid glycosyl transferase to production of calendula glycoside E. The invention discovers that the oleanolic acid glycosyl transferase UGT73C10 can catalyze hydroxyl at C3 site of oleanolic acid and glucoside of uridine-5 '-diphosphate glucuronic acid (UDP-glucuronic acid) to complete glucoside transfer so as to synthesize calendula glycoside E for the first time. Experiments show that glycosyl transferase UGT73C10 is expressed in escherichia coli in a heterologous mode, and oleanolic acid is efficiently converted into calendula glycoside E. The research provides a new research thought and an application direction for producing calendula glycoside E through efficient biotransformation of the engineering strain.

The invention relates to the technical field of raw materials of skin care products, and particularly discloses a preparation method of a paeonia lactiflora root extract, which comprises the following steps: step 1), putting paeonia lactiflora roots into an extracting solution, and heating and pressurizing for extraction to obtain an extract; step 2), filtering the extract, taking filtrate, and concentrating and drying the filtrate to obtain a Chinese herbaceous peony root extract rich in beta-pentagalloylglucose and oleanolic acid; the extracting solution is a compound of an ethanol water solution, acetic acid, acetone and chloroacetaldehyde. The method has the advantages that more beta-pentagalloylglucose and oleanolic acid are extracted from the paeonia lactiflora roots, so that the whitening and antioxidant effects of the paeonia lactiflora root extract are further improved.

The invention relates to a method for purifying oleanolic acid from Tibetan capillary artemisia, which comprises the following steps: (1) purifying a Tibetan capillary artemisia crude extract by alkali dissolution and acid precipitation to obtain an oleanolic acid crude product, and cleaning the oleanolic acid crude product to obtain a sample after alkali dissolution and acid precipitation; (2) dissolving the sample subjected to alkali dissolution and acid precipitation to obtain a dissolved solution; and (3) refluxing and decolorizing the dissolved solution with activated carbon, filtering, and crystallizing the filtrate to obtain the oleanolic acid product.

The invention discloses an oleanolic acid acylhydrazone derivative as well as a preparation method and application thereof. The oleanolic acid acylhydrazone derivatives have a structural formula shown in the following formula I. In the structural formula, R is selected from halogen-substituted pyridine and hydroxyl-substituted naphthyl. The invention provides a series of oleanolic acid acylhydrazone derivatives with novel structures, and the oleanolic acid acylhydrazone derivatives have relatively good inhibition effects on human breast cancer cells MDA-MB-231 and human pancreatic cancer cells Mia paca-2, and can be used for preparing an anti-cancer drug. The compound can be used for preparing anti-tumor drugs.

The invention relates to application of saponin in inhibition of penicillium. The saponin is at least one of oleanolic acid type saponin, hedera helix type saponin and phytolaccae acid type saponin. On the basis, the citrus preservative can be used for citrus preservation to improve the quality and prolong the shelf life of citrus so as to guarantee the commodity value of fruits.

The present application belongs to the technical field of biological medicine, and particularly relates to a composition for treating atopic dermatitis and application thereof. The mass ratio of each component of the composition is: notoginseng extract: artemether: wujin glycoside: oleanolic acid = (2-4):(2-4):(1-2):(1-2). The composition has the effects of anti-inflammation and anti-itching, and can treat atopic dermatitis, eczema and other diseases. The active ingredients of the composition are all of plant origin, have good safety and the treatment effect on atopic dermatitis, and have a low dosage. The components can achieve a synergistic effect, and show excellent effects. The composition can be applied to functional skin care products (cream, emulsion, essence) and external drugs (ointment).

The invention discloses an oleanolic acid liposome. The oleanolic acid liposome is prepared from soybean lecithin, sodium tauroursodeoxycholate and oleanolic acid in a mass ratio of (10-20): (0.5-2.5): (0.5-2.5). The sodium tauroursodesoxycholate replaces cholesterol to serve as a raw material of the oleanolic acid liposome, so that the oleanolic acid liposome has higher storage stability and gastrointestinal tract stability, the storage period and the residence time and action time of oleanolic acid in vivo can be prolonged, the oleanolic acid liposome has smaller particle size, and the bioavailability of the oleanolic acid liposome is improved. The oleanolic acid lipidosome prepared by the preparation method disclosed by the invention can increase the absorption rate, and also improves the blood fat reducing and blood sugar reducing effects of the oleanolic acid lipidosome through the biological activity of the tauroursodesoxycholic acid, so that the oleanolic acid lipidosome has a wider practical application value.

The invention discloses a UHPLC-MS / MS (Ultra High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry)-based multi-component content determination method for a compound radix scutellariae throat-clearing capsule, which is characterized in that a multi-component simultaneous quantitative analysis method is established by adopting a UHPLC-MS / MS technology, and the quality of the compound radix scutellariae throat-clearing capsule is evaluated from the quantitative aspect. 21 components including p-hydroxybenzaldehyde, vanillin, caffeic acid, syringaldehyde, citric acid, tanshinol, chrysin, baicalein, genistein, quercetin, chlorogenic acid, neochlorogenic acid, rosmarinic acid, harpagide, puerarin, apigenin-7-O-beta-D-glucoside, baicalin, oleanolic acid, scutellarin, glycyrrhetinic acid and harpagoside are selected as quantitative indexes; and determining the content of the compound radix scutellariae throat-clearing capsule. According to the method, the contents of 21 components in the compound radix scutellariae throat-clearing capsule can be accurately measured at the same time within 14 min, and the method has good precision and stability.