Ultrasonic-microwave synergetic extracted cornel iridoid glycoside, as well as extraction and determination method thereof

Abstract

The invention provides a method for extracting cornel iridoid glycoside by virtue of ultrasonic-microwave synergetic extraction. The method comprises the following steps: (1) preparing cornus officinalis coarse powder; (2) adding the cornus officinalis coarse powder into a 80% methanol solution in a material-liquid ratio of (1:5)-(1:25), sealing, weighing and determining the weight, connecting a condensing device, and extracting for 3-15 minutes with microwave power of 300-700W and ultrasonic powder of 120-600W to obtain an extract; (3) carrying out suction filtration on the extract to obtain filtrate, keeping the constant volume, filtering, concentrating, drying and purifying to obtain the cornel iridoid glycoside. A method for determining the cornel iridoid glycoside comprises the following steps: a, preparing a mixed reference substance solution; b, preparing a test substance solution; c, determining the contents of morroniside, loganin, sweroside and cornel iridoid glycoside in the mixed reference substance and the test substance; d, respectively calculating the contents of the four glycosides according to the result of liquid chromatographic analysis.

Description

technical field [0001] The invention belongs to the field of extraction and determination of iridoid glycosides of cornus officinalis, in particular to iridoid glycosides of cornus officinalis co-extracted by ultrasonic-microwave and its extraction and determination method. Background technique [0002] Cornus officinalis Sieb.et Zucc. is the dry and mature pulp of Cornus officinalis. It tastes sour, astringent, and slightly warm in nature. It returns to the liver and kidney meridian. , Lumbago and knee pain, impotence, nocturnal emission, enuresis, frequent urination, metrorrhagia, profuse sweating, prostration, internal heat and thirst. It is mainly produced in Shaanxi, Zhejiang, Henan and other provinces in my country, and it is also cultivated in Sichuan, Anhui, Shandong, Shanxi and other places. Cornus officinalis is the raw material of commonly used Chinese patent medicines "Liu, Bawei Dihuang Pills" and "Qiju Dihuang Pills". In recent years, scholars at home and abr...

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Problems solved by technology

[0004] In the prior art, not only the extraction method of iridoid glycosides from Cornus officinalis cannot meet the requirements, but also the content of the effective substances in the product obtained after extraction cannot be rapidly determined. Effective simultaneous separation and determination, the HPLC chromatographic method for the determination of multiple active ingredients in Cornus officinalis has low peak separation, and each peak does not completely reach the baseline separation

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