17 results about "Anion radicals" patented technology

The present application belongs to the field of organic photo-functional materials and biomedical materials, and particularly relates to a phenanthraquinone-based covalent organic framework material, a nano-preparation containing the same, a preparation method thereof and biomedical applications. In the present application, a Schiff base condensation reaction is carried out between a bis(formylphenyl) monomer with a phenanthraquinone group or an aza-phenanthraquinone group and N,N,N',N'-tetra(p-aminophenyl)-p-phenylenediamine, and through reversible covalent connection of aldehyde groups and amino groups, the phenanthraquinone-based covalent organic framework material is prepared, and it is found that the phenanthraquinone-based covalent organic framework material can efficiently generate superoxide anion radicals under 808 nm near-infrared light irradiation, thereby destroying the intracellular redox homeostasis, selectively accelerating NADPH consumption, promoting excessive formation of disulfide bonds, and leading to induction of tumor cells to undergo disulfide death. The phenanthraquinone-based covalent organic framework material of the present application provides a new strategy for solving the problem of drug resistance in tumor treatment, and has a broad application prospect.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The invention provides photocatalyst nanoparticles for air sterilization and a preparation method of the photocatalyst nanoparticles. The preparation method of the photocatalyst nano-particles for air sterilization comprises the steps of preparing nitrogen-doped titanium dioxide precursor sol, preparing nitrogen-doped titanium dioxide nano-particles, preparing a second mixed system and preparing Ag / Cu bimetallic loaded nitrogen-doped titanium dioxide nano-particles. According to the preparation method, the nitrogen doping amount, the calcining temperature and the heat preservation time are synergistically optimized, so that the obtained nitrogen-doped TiO2 nanoparticles have relatively high crystallinity and relatively large specific surface area while keeping stable existence of an anatase phase, thereby improving the separation efficiency of photo-induced electron-hole pairs and the number of surface active sites; under the irradiation of visible light, more hydroxyl free radicals, superoxide anion free radicals and peroxides can be generated, and bacteria, viruses and volatile organic compounds in the air can be efficiently oxidized, decomposed and inactivated. The invention also provides an air filtration nanofiber membrane.

The invention belongs to the technical field of nano-catalyst antibiosis, and particularly relates to a zinc-based nitrogen-doped carbon-loaded metal palladium nano-enzyme as well as a preparation method and application thereof. The ZnNC-loaded metal palladium nano-enzyme is prepared by adopting a deposition-precipitation method which is simple to operate, taking ZnNC as a carrier and taking metal palladium nano-particles as an active center. The nano-enzyme has excellent oxidase-like activity, can catalyze O2 to generate hydroxyl free radicals (. OH), singlet oxygen (< 1 > O2) and superoxide anion free radicals (O2 <->) so as to kill bacteria and achieve an antibacterial effect, and has a remarkable antibacterial effect on escherichia coli under an acidic condition.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

The present application provides a kind of fluorine boron compound, the fluorine boron compound contains anthraquinone group, can generate strong oxidizing anion radical after photoexcitation efficiently, thus has lower half inhibitory concentration, stronger inhibitory effect, has stronger phototoxicity, can kill cancer cells in shorter time;Meanwhile, the superoxide anion radical generated can specifically oxidize FADH2 coenzyme in mitochondrial electron transport chain, by oxidizing its isoalloxazine ring structure to cause coenzyme inactivation, then mitochondrial electron transport process, and inhibit cancer cell energy metabolism, so as to effectively inhibit the proliferation activity of cancer cells.

This invention belongs to the technical field of organometallic luminescent materials and photosensitizers, specifically relating to a triphenylamine-containing heteropolymer luminescent iridium(III) complex capable of generating reactive oxygen species, its preparation method, and its applications. The iridium(III) complex has the structure shown in formula (I), [Ir(L1)2(L2)]PF6, where L1 is 1-(4-(diphenylamino)phenyl)isoquinoline and L2 is 2-[3-methyl-1-(4-(diphenylamino)phenyl)-1H-1,2,4-triazol-5-yl]pyridine. The complex can be prepared by first preparing a chlorobridged iridium dimer intermediate, then coordinating it with an auxiliary ligand and followed by anion exchange. This complex exhibits luminescent properties in both dilute solutions and solid states, and displays certain solvent-dependent luminescence behavior. Under LED white light irradiation, the complex can effectively generate reactive oxygen species (ROS). Detection using DCFH-DA, HPF, and DHR123 probes indicates that it possesses the ability to generate total ROS, hydroxyl radicals, and superoxide anion radicals. The complex can be used to prepare luminescent materials, ROS-generating materials, and photodynamic photosensitive materials.

The invention belongs to the technical field of microbial fermentation, and particularly relates to saccharomycetes SY and application thereof in preparation of a codonopsis pilosula fermentation product which is high in in-vivo and in-vitro bioavailability and capable of improving gastrointestinal motility. The codonopsis pilosula fermented product is prepared by fermenting codonopsis pilosula with saccharomycetes SY. The content of lobetyolin in the pilose asiabell root leavening prepared by fermenting pilose asiabell root through the saccharomycetes SY is high, in-vitro digestion experiments prove that the removal rate of lobetyolin, total flavonoids and superoxide anion free radicals and the bioaccessibility of the total reducing capacity are remarkably improved, and rat experiments find that the pilose asiabell root leavening can promote gastrointestinal motility and improve the bioavailability of the pilose asiabell root. Intestinal mucosa regeneration and barrier repair are promoted; the content of effective components such as luteolin 7-glucosid-3 '-xyloside capable of enhancing immune cell activity, regulating inflammatory factor balance, protecting cells from oxidative stress injury and improving gastrointestinal digestion and absorption functions and energy metabolism in serum is increased, and absorption of the effective components in blood is promoted.

The application discloses a hybrid synergistic electrochemiluminescence quencher, a preparation method and application thereof. Dopamine, zinc ions and 2-methyl imidazole are used as raw materials to synthesize polydopamine and a hybrid material of a zeolitic imidazolate framework by one-pot synthesis under alkaline conditions. The introduction of the electropositive zeolitic imidazolate framework can greatly eliminate the strong electronegativity of the polydopamine and simultaneously endow the quencher with good porosity. The quencher prepared by the application is nanospheres, has a large specific surface area and weak electronegativity, and exhibits good scavenging capacity for free radicals, especially electrogenerated anionic radicals, and has a high quenching efficiency on electrochemiluminescence. After the quencher is labeled with an antibody, the quencher is used for constructing an electrochemiluminescence immunosensor for ochratoxin A. The electrochemiluminescence immunosensor prepared by the application has a wide detection range, a low detection limit and good stability in the detection of ochratoxin A, and has a wide application prospect in the fields of electrochemiluminescence technology and the like.

The application relates to an earth magnetic field sewage treatment equipment and a water body treatment method thereof, the water body treatment method comprising the following steps: immersing the box into water and electrifying, the electron of H atom in water molecules is subjected to transition, the water molecules are ionized and decomposed, atomic H and atomic O are generated; a functional film layer made of low-dimensional carbon nanomaterials absorbs ultraviolet energy in sunlight, when the ultraviolet energy is greater than the forbidden band width of the functional film layer, intrinsic excitation is generated, an electron-hole pair is generated, the electron and the hole both enter water, and a photochemical reaction occurs to generate superoxide anion radical O2-, hydroxyl radical.OH, monatomic H and monatomic O; the water body is purified through the superoxide anion radical O2-, the hydroxyl radical.OH, the monatomic H and the monatomic O. Different pollutions can be effectively removed to cope with different pollution conditions.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a microbial preparation and application of the microbial preparation in preparation of an astragalus mongholicus fermented product with the effects of reducing blood sugar and blood fat and high bioaccessibility. The microbial preparation is prepared from lactobacillus mucus fermentation FJ701 and leuconostoc mesenteroides XD054. The preparation method comprises the following steps: performing enzymolysis on astragalus membranaceus, inoculating a microbial preparation and fermenting to obtain an astragalus membranaceus fermented product; the Astragalus mongholicus fermented product has high content of total polyphenol, total saponin and polysaccharide, and can inhibit pancreatic lipase activity and alpha-glucosidase activity, and in vitro digestion tests find that the scavenging rates of formononin, total polyphenol, total saponin and superoxide anion free radicals are high in bioaccessibility; rat experiments find that the concentration of calycosin-7-glucoside in blood in the astragalus membranaceus fermentation product is high, various active ingredients such as syringin 3-robiniobioside in serum are increased, and the anti-inflammatory and antioxidant capacities are improved.

The application belongs to the technical field of biological medicine, and particularly relates to a coumarin hydrazone derivative with antioxidant activity and application thereof. The chemical structural formula of the coumarin derivative is shown as formula (I), wherein R is an aromatic ring containing different substituents. The in vitro antioxidant experiment results show that most of the derivatives exhibit certain scavenging capacity on DPPH, ABTS, superoxide anion and hydroxyl radicals, and have certain reducing capacity. Notably, the derivatives 5a-5d, 5i-5k, 5m and 5n have better scavenging effect on superoxide anion radicals than or equivalent to vitamin C. The method for preparing the coumarin hydrazone derivative has the advantages of simple operation, convenient post-treatment, high product separation yield and the like. The coumarin compound can be used for preparing an antioxidant.

This invention discloses a method for preparing pear peel polyphenols based on thiols and their antioxidant applications, belonging to the field of natural product extraction and functional foods. The preparation of pear peel polyphenols according to this invention includes the following steps: adding acidified methanol solution and toluene-α-thiol solution to pear peel powder, and ultrasonically extracting at 40-45℃ for 20-30 min. After extraction, the supernatant is collected and concentrated by rotary evaporation to obtain a pear peel polyphenol extract. This pear peel polyphenol extract is rich in chlorogenic acid, cryptochlorogenic acid, arbutin, and other phenolic components; it exhibits significant scavenging ability against hydroxyl radicals, superoxide anion radicals, DPPH radicals, and ABTS radicals, demonstrating good antioxidant activity; it can be used as a natural antioxidant to replace synthetic antioxidants in food systems; effectively realizing the high-value utilization of pear processing by-products (pear peel).

The invention belongs to the crossing field of organic optical functional materials and biomedical materials, and particularly relates to a phenanthrenequinone-based covalent organic framework material, a nano preparation containing the phenanthrenequinone-based covalent organic framework material as well as a preparation method and biomedical application of the phenanthrenequinone-based covalent organic framework material. The phenanthrenequinone-based covalent organic framework material is prepared by carrying out Schiff base condensation reaction on a phenanthrenequinone-based or aza-phenanthrenequinone-based bis (formyl phenyl) monomer and N, N, N ', N'-tetra (p-aminophenyl) p-phenylenediamine and carrying out reversible covalent connection on an aldehyde group and an amino group. And it is found that superoxide anion free radicals can be efficiently generated under irradiation of 808 nm near-infrared light, so that the redox steady state in cells is destroyed, NADPH consumption is selectively accelerated, disulfide bond excessive formation is promoted, and tumor cells are induced to generate disulfide death. The phenanthrenequinone-based covalent organic framework material provides a new strategy for solving the problem of drug resistance in tumor treatment, and has a wide application prospect.

The present application provides a kind of NIR-II fluorescent emission and I type photodynamic activity of nitrogen hetero fluorine boron fluorescent molecule and its preparation method, nitrogen hetero fluorine boron fluorescent molecule has good light absorption in near infrared (NIR) region, and its emission peak is extended to near infrared two (NIR-II) and exhibits 13% of super high NIR-II fluorescence quantum yield.Meanwhile, the photosensitizer prepared by using nitrogen hetero fluorine boron fluorescent molecule can also effectively produce I type active oxygen species-super oxide anion radical under 808nm laser excitation.Based on this, the NIR-II fluorescence of nitrogen hetero fluorine boron fluorescent photosensitizer can realize high-resolution tumor fluorescence imaging, for deep tumor tissue precise, efficient NIR-II fluorescence imaging mediated I type photodynamic therapy.

This invention discloses a photosensitizing lipid nanoparticle based on a multi-resonance core framework, its preparation method, and its antibacterial application, belonging to the field of biomedical materials technology. The photosensitizer structure in this invention is characterized by a multi-resonance fused-ring boron nitrogen as the molecular core framework, with a cleverly introduced triazine electron-withdrawing group on the periphery. This enhances the stretching vibration of the molecule, increases its collision probability with oxygen molecules, accelerates the electron transfer process, and thus promotes the generation of type I reactive oxygen species (ROS). The lipid nanoparticles BN-TRZ@NPs prepared by ultrasound have an absorption wavelength in the range of 550-680 nm. Under white light irradiation, BN-TRZ@NPs can efficiently generate type I ROS (superoxide anion radicals) and exhibit excellent sterilization effects against methicillin-resistant Staphylococcus aureus (MRSA). This invention is the first to apply a photosensitizer based on a multi-resonance structure to biomedical research in the field of antibacterial applications, providing a new approach for the biomedical application of next-generation biotherapeutic photosensitizer molecules.

This invention discloses a method for deep treatment of pharmaceutical wastewater based on peroxide removal mediated by interfacial electron migration of immobilized materials. Using porous, highly stable, and mechanically strong SiC foam as a carrier, highly active components such as CuBi₂O₄, CuCo₂O₄, Cu / MnO₂, and Fe / C₃N₄ are loaded in situ and with high dispersion at the interface. This effectively solves the problems of easy agglomeration, difficulty in separation, and low recovery efficiency of powdered active ingredients. By adding hydroxylamine hydrochloride and ascorbic acid to enhance interfacial electron transfer of active ingredients, the method addresses the issues of slow reduction rates of high-valence metal ions and difficulty in regenerating free electrons. This achieves efficient activation of hydrogen peroxide, monopersulfate, persulfate, and ozone, mediating the generation of highly oxidizing hydroxyl radicals, sulfate radicals, superoxide anion radicals, and singlet oxygen, thus realizing the efficient oxidation of recalcitrant organic matter in drinking water and wastewater.