Protein capable of antioxidant activity separated from acaleph and application thereof

An antioxidant activity, jellyfish technology, applied in the field of marine biology, can solve the problem of low added value, and achieve the effect of low production cost and high antioxidant activity

Inactive Publication Date: 2007-10-17
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the utilization of jellyfish resources is only after simple processing at the present stage. As food, the added value is not high

Method used

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  • Protein capable of antioxidant activity separated from acaleph and application thereof
  • Protein capable of antioxidant activity separated from acaleph and application thereof
  • Protein capable of antioxidant activity separated from acaleph and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Break a certain amount of jellyfish with an ultrasonic breaker, add 0.5 times the weight / volume ratio of 5mM, pH4 phosphate buffer solution (PB), put it at 2°C, and soak for 0.5h;

[0029] (2) Centrifuge at 10,000 rpm for 30 minutes at 4°C, and take the supernatant; the residue is leached twice with 5mM phosphate buffer solution (PB) with a weight / volume ratio of 0.5 times, pH 4, and soaked at 4°C 0.5h; centrifuge at 10000rpm at 4°C for 30min; combine the supernatants from the two centrifugations;

[0030] (3) with Coomassie Brilliant Blue staining method (this method is selected from "Protein Molecular Basis", edited by Tao Weisun et al., Higher Education Press), taking bovine serum albumin as a standard, measuring the absorbance of jellyfish protein at 595nm (A 595 ), according to Lambert Beer's law, and then calculate the concentration of aequorin in the supernatant (C mg / ml ).

[0031] (4) with gel filtration chromatography (this method is selected from "Prote...

Embodiment 2

[0033] (1) Break a certain amount of jellyfish with an ultrasonic breaker, add 2.0 times the weight / volume ratio of 10mM, pH6 phosphate buffer solution (PB), put it at 4°C, and soak for 1h;

[0034] (2) Centrifuge at 15,000 rpm for 20 minutes at 4°C, and take the supernatant; the residue is leached twice with 10mM phosphate buffer solution (PB) with a weight / volume ratio of 1.5 times, pH 6, and soaked at 4°C 1h; centrifuge at 15000rpm at 4°C for 20min; combine the supernatants from the two centrifugations;

[0035] (3) with Coomassie Brilliant Blue staining method (this method is selected from "Protein Molecular Basis", edited by Tao Weisun et al., Higher Education Press), taking bovine serum albumin as a standard, measuring the absorbance of jellyfish protein at 595nm (A 595 ), according to Lambert Beer's law, and then calculate the concentration of aequorin in the supernatant (C mg / ml ).

[0036] (4) with gel filtration chromatography (this method is selected from "Protein...

Embodiment 3

[0038] (1) Break a certain amount of jellyfish with an ultrasonic breaker, add 5.0 times the weight / volume ratio of 20mM, pH8 phosphate buffer solution (PB), put it at 4°C, and soak for 2h;

[0039] (2) Centrifuge at 20,000 rpm for 10 minutes at 4°C, and take the supernatant; the residue is leached twice with 20mM phosphate buffer solution (PB) with a weight / volume ratio of 1.5 times, pH8, and soaked at 4°C 1h; centrifuge at 20000rpm at 4°C for 10min; combine the supernatants from the two centrifugations;

[0040] (3) with Coomassie Brilliant Blue staining method (this method is selected from "Protein Molecular Basis", edited by Tao Weisun et al., Higher Education Press), taking bovine serum albumin as a standard, measuring the absorbance of jellyfish protein at 595nm (A 595 ), according to Lambert Beer's law, and then calculate the concentration of aequorin in the supernatant (C mg / ml ).

[0041](4) with gel filtration chromatography (this method is selected from "Protein M...

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Abstract

The present invention relates to halobios, specifically two kinds of acaleph proteins capable of antioxidant activity, its separation step is: after grinding acaleph and breaking cell, filling into phosphate buffer solution, marinating 0.5 to 2h at 2 to 6DEG C; centrifuging 10 to 30min with rotate speed 10000 to 20000rpm, taking the supernatant; marinating with phosphate buffer solution again, centrifuging 10 to 30min with rotate speed 10000 to 20000rpm; combining the two times centrifugal supernatant; separating the combined supernatant by gel filtration chromatography to obtain two kinds of acaleph proteins; they can inhibit superoxide anion radical( O2-.), hydroxyl radical (. OH) generating, prevent red blood cell hemolysis due to hydroxide peroxidation and liver lipid peroxidation; belonging to natural antioxidants, high antioxidant activity, no poison to human and livestock; the present invention uses abundant acaleph halobios resource at the same time, for low-value of the resources to provide a new path for high value development.

Description

[0001] This application is a divisional application of Publication No. CN1752101A, its invention name: protein with antioxidant activity isolated from jellyfish and its application, application number: 200410050481.3, filing date: 20040922. technical field [0002] The invention relates to marine organisms, specifically two types of jellyfish proteins with anti-oxidation activity, which can be used as a drug source for anti-aging drugs, and at the same time provide a new way for the development of natural food additives. Background technique [0003] Reactive oxygen free radicals have a huge damage effect on the body, such as causing protein damage, enzyme inactivation, membrane peroxidation, leading to aging, tumors, atherosclerosis and many other diseases. Synthetic butylated hydroxytoluene (BHT), butyl hydroxymethoxy (BHA), propylene glycol (PG), etc. have a good effect on scavenging active oxygen free radicals, and are often used as food additives. However, recent studies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23L1/305A61K35/60A61P39/06A23L33/17
Inventor 李鹏程于华华刘希光邢荣娥刘松郭占勇王丕波李翠萍
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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