Preparation method of antioxidative peptide of hairtail

Abstract

The invention discloses a preparation method of an antioxidative peptide of a hairtail. The preparation method comprises the following steps of 1, adding water into hairtails, and mincing the hairtails into hairtail paste, 2, adding the hairtail paste into an enzymolysis tank, and adding endoprotease into the hairtail paste for hydrolysis, wherein the mass of the endoprotease is 0.2 to 0.9% of the mass of the hairtails, 3, carrying out centrifugation of a hydrolysate obtained by the step 2, and collecting a supernatant, 4, carrying out microfiltration of the supernatant, adding the micro-filtrated supernatant into the enzymolysis tank, and adding excision protease into the enzymolysis tank for hydrolysis, wherein the mass of the excision protease is 0.1 to 0.9% of the mass of the hairtails, 5, in the hydrolysis, pumping an enzymatic hydrolysate into an ultrafiltration membrane separator from the enzymolysis tank so that the enzymatic hydrolysate can be circulated at a flow of 0.1 to 0.5L / min, and 6, collecting an ultrafiltration membrane permeate, carrying out reduced pressure concentration until a volume of the ultrafiltration membrane permeate is 1 / 10 to 1 / 5 of the original volume, keeping a temperature of -18 to -20 DEG C for a night, and carrying out freeze drying at a temperature of -60 to -65 DEG C under the pressure of 0.005 to 0.0054 mbar to obtain the antioxidative peptide of hairtails. The antioxidative peptide of hairtails has strong antioxidation activity, a hydroxyl radical removal rate of 82.0%, a superoxide anion radical removal rate of 85.2% and a DPPH radical removal rate of 61.5%.

Description

technical field [0001] The invention relates to the field of biotechnology, and specifically refers to a method for preparing antioxidant peptides by using hairtail fish as raw material, hydrolyzing hairtail protein with compound enzymes, and coupling enzymatic hydrolysis and membrane separation. technical background [0002] Hairtail, also known as saury and octopus, is a species of hairtail of the order Perciformes. It is a kind of economic fish with high production in my country's coastal areas. It is delicious and is favored by consumers at home and abroad. Hairtail has high nutritional value, and has a certain dietary effect on post-ill physical weakness, postpartum milk deficiency and traumatic bleeding. Traditional Chinese medicine believes that it has the effects of harmonizing the appetite, warming the stomach and tonifying deficiency, moisturizing the skin and beautifying. The protein content of hairtail is high, and hairtail protein is a kind of complete protein,...

AI Technical Summary

Problems solved by technology

The fly in the ointment is that because only one enzyme is used to hydrolyze the protein, and in the process of preparing antioxidant peptides, the molecular weight cut-off of the ultrafiltration membrane used is 5000Da-10000Da; Oxygen anion free radical scavenging rate is only 24.8-28.2%; DPPH free radical scavenging rate is only 40.2-47.1%