Preparation method of antioxidative peptide of hairtail

A technology of antioxidant peptide and hairtail, applied in the biological field, can solve the problem of low antioxidant capacity of antioxidant peptide, only 40.2-47.1%, and achieve high antioxidant activity, low production cost, and simple process

Inactive Publication Date: 2012-10-24
NINGBO WUSHENG CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fly in the ointment is that because only one enzyme is used to hydrolyze the protein, and in the process of preparing antioxidant peptides, the molecular weight cut-off

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Remove the head, tail and viscera of the hairtail, wash and weigh 1.0kg hairtail, add 3.0kg of water, and mince it into a uniform fish paste; put the fish paste into an enzymatic hydrolysis tank, adjust the temperature to 60°C, and adjust the pH to 7.5, based on hairtail mass, add 0.9% hairtail mass Alcalase alkaline protease, stir and hydrolyze for 12 hours; centrifuge the hydrolyzate: 4500rpm, 4°C, 12 minutes, and take the supernatant. Put the supernatant through a 0.45um (micrometer) microporous membrane for microfiltration and then put it into an enzymatic hydrolysis tank. Add 0.9% Flavourzyme flavor protease based on hairtail mass, adjust the temperature to 50°C, adjust the pH to 6.5, and stir Hydrolysis for 10 hours; during the above hydrolysis process, the enzymatic hydrolysis solution (also called hydrolysis solution) is pumped from the enzymatic hydrolysis tank into the ultrafiltration membrane separator, so that the enzymatic hydrolysis solution circulates at a...

Embodiment 2

[0027] Remove the head, tail and viscera of the hairtail, wash and weigh 2.0kg of hairtail, add 5.0kg of water, and grind it into a uniform fish paste; put the fish paste into an enzymatic hydrolysis tank, adjust the temperature to 50°C, and adjust the pH to 7.0, based on hairtail mass, add 0.8% Neutrase neutral protease, stir and hydrolyze for 10 hours; centrifuge the hydrolyzate at 4500rpm, 4°C, 14 minutes, and take the supernatant. Put the supernatant through a 0.45um microporous membrane for microfiltration and then put it into an enzymatic hydrolysis tank. Add 0.5% carboxypeptidase based on hairtail mass, adjust the temperature to 40°C, adjust the pH to 5.5, and stir for 9 hours; In the above hydrolysis process, the enzymolysis solution is pumped from the enzymolysis tank into the ultrafiltration membrane separator, so that the enzymolysis solution circulates at a flow rate of 0.25L / min, and the pressure of the ultrafiltration membrane is adjusted to 0.09Mpa. The molecula...

Embodiment 3

[0029]Remove the head, tail and viscera of the hairtail, wash and weigh 2.5kg of hairtail, add 5.0kg of water, and grind it into a uniform fish paste; put the fish paste into an enzymatic hydrolysis tank, adjust the temperature to 50°C, and adjust the pH to 6.5, based on hairtail mass, add 0.5% papain, stir and hydrolyze for 14 hours; centrifuge the hydrolyzate at 4500rpm, 4°C, 13 minutes, and take the supernatant. Put the supernatant through a 0.45um microporous membrane for microfiltration and then put it into an enzymatic hydrolysis tank. Add 0.8% aminopeptidase based on hairtail mass, adjust the temperature to 45°C, adjust the pH to 7.5, and stir for 8 hours; In the above enzymolysis process, the enzymolysis solution is pumped into the ultrafiltration membrane separator from the enzymolysis tank, so that the enzymolysis solution circulates at a flow rate of 0.4L / min, and the pressure of the ultrafiltration membrane is adjusted to be 0.08Mpa. The molecular weight cut-off is...

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Abstract

The invention discloses a preparation method of an antioxidative peptide of a hairtail. The preparation method comprises the following steps of 1, adding water into hairtails, and mincing the hairtails into hairtail paste, 2, adding the hairtail paste into an enzymolysis tank, and adding endoprotease into the hairtail paste for hydrolysis, wherein the mass of the endoprotease is 0.2 to 0.9% of the mass of the hairtails, 3, carrying out centrifugation of a hydrolysate obtained by the step 2, and collecting a supernatant, 4, carrying out microfiltration of the supernatant, adding the micro-filtrated supernatant into the enzymolysis tank, and adding excision protease into the enzymolysis tank for hydrolysis, wherein the mass of the excision protease is 0.1 to 0.9% of the mass of the hairtails, 5, in the hydrolysis, pumping an enzymatic hydrolysate into an ultrafiltration membrane separator from the enzymolysis tank so that the enzymatic hydrolysate can be circulated at a flow of 0.1 to 0.5L/min, and 6, collecting an ultrafiltration membrane permeate, carrying out reduced pressure concentration until a volume of the ultrafiltration membrane permeate is 1/10 to 1/5 of the original volume, keeping a temperature of -18 to -20 DEG C for a night, and carrying out freeze drying at a temperature of -60 to -65 DEG C under the pressure of 0.005 to 0.0054 mbar to obtain the antioxidative peptide of hairtails. The antioxidative peptide of hairtails has strong antioxidation activity, a hydroxyl radical removal rate of 82.0%, a superoxide anion radical removal rate of 85.2% and a DPPH radical removal rate of 61.5%.

Description

technical field [0001] The invention relates to the field of biotechnology, and specifically refers to a method for preparing antioxidant peptides by using hairtail fish as raw material, hydrolyzing hairtail protein with compound enzymes, and coupling enzymatic hydrolysis and membrane separation. technical background [0002] Hairtail, also known as saury and octopus, is a species of hairtail of the order Perciformes. It is a kind of economic fish with high production in my country's coastal areas. It is delicious and is favored by consumers at home and abroad. Hairtail has high nutritional value, and has a certain dietary effect on post-ill physical weakness, postpartum milk deficiency and traumatic bleeding. Traditional Chinese medicine believes that it has the effects of harmonizing the appetite, warming the stomach and tonifying deficiency, moisturizing the skin and beautifying. The protein content of hairtail is high, and hairtail protein is a kind of complete protein,...

Claims

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Application Information

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IPC IPC(8): C12P21/06B01D61/14
Inventor 吴惠岳靳挺周斌武玉学
Owner NINGBO WUSHENG CHEM
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