Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrode for active oxygen species and sensor using the electrode

A technology for reactive oxygen species and sensors, which is applied in the field of electrodes for reactive oxygen species and sensors using the electrodes, and can solve problems such as enzyme inactivation

Inactive Publication Date: 2005-04-06
武林 敬 +2
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, the enzymes used are inactivated within days

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrode for active oxygen species and sensor using the electrode
  • Electrode for active oxygen species and sensor using the electrode
  • Electrode for active oxygen species and sensor using the electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0091] The present invention will be described in more detail in the following reference examples, examples and test examples, but these examples should not be regarded as limiting the present invention.

reference example 1

[0093] 5,10,15,20-tetrakis(3-thienyl)porphyrin (H 2 Synthesis of T3ThP)

[0094] A 100ml round bottom flask was charged with 50ml propionic acid, 2.0ml 3-thiopheneacetaldehyde and 1.4ml pyrrole. While stirring the mixture, it was refluxed at 160° C. for one hour. After reflux, the reaction product was cooled to room temperature, cooled with ice cubes, and 200 ml of cold methanol was added. The mixture was filtered with suction. The filtrate was washed with methanol and purified by silica gel chromatography (developing solvent: chloroform). The solvent was evaporated to dryness and the solid was recrystallized, and then dried under reduced pressure to obtain H in the form of black powder crystals. 2 T3ThP (yield: 0.63 g, 19%). The product was measured using an ultraviolet-visible spectrophotometer (UV-2100, manufactured by Shimadzu Corporation) and 1 H-NMR identification. The results are shown in Table 1 and Table 2.

reference example 2

[0096] 5,10,15,20-tetrakis(2-aminophenyl)porphyrin (H 2 Synthesis of T2AmP)

[0097] Charge 500 ml of propionic acid into a 2L four-necked flask with a reflux condenser. After adding 25 g of 2-nitrobenzaldehyde, the mixture was heated while being stirred and refluxed at 110°C. 12 ml of pyrrole were added, and the mixture was refluxed at the boiling point for 30 minutes. After adding 50 ml of chloroform, the mixture was cooled with ice and filtered off with suction. The filtrate was washed with 400ml of chloroform, and dried under reduced pressure (100°C, 6 hours, 0.1kPa), thereby obtaining the precursor 5,10,15,20-tetrakis(2-nitrophenyl ) porphyrin (H 2 T2NO 2 P) (Yield: 5.0 g, 14%).

[0098] Charge 300ml of 12N HCL into a 2L four-necked flask. After adding the above-mentioned synthetic 5,10,15,20-tetrakis(2-nitrophenyl)porphyrin (H 2 T2NO 2 P) and 20.0 g of tin(II) chloride dihydrate, the mixture was heated at 65°C-70°C for 30 minutes. Aqueous ammonia was gradually ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

An electrode for active oxygen species, characterized in that it comprises an electroconductive member and, formed thereon, a polymer film prepared from a metal porphirin. The electrode for active oxygen species allows the determination, both in vivo and in vitro, of active oxygen species such as a superoxide anion radical and hydrogen peroxide, or OH, and other radical active species (such as NO, ONOO-), and thus can be used for specifying various diseases and for examining active oxygen species present in a food or water such as a tap water and a sewage.

Description

technical field [0001] The present invention relates to an electrode for active oxygen species in a living body and a sensor for measuring the concentration of active oxygen species using the electrode, and the active oxygen species is, for example, a peroxide anion radical (O 2 - ·). More particularly, the present invention relates to an electrode for measuring reactive oxygen species and a sensor for measuring the concentration of reactive oxygen species suitable for in vivo measurement without using a large amount of enzyme and without causing problems of enzyme inactivation. Background technique [0002] Peroxide anion radicals (O 2 - ) is produced in the body by oxidizing xanthine or hypoxanthine etc. into uric acid by xanthine-xanthine oxidase (XOD) and reducing oxygen by hemoglobin. Peroxide anion free radicals play an important role in the synthesis of physiologically active substances, bactericidal effect and aging process. Various reactive oxygen species deriv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00C08G61/12C08G73/06C11D3/00C11D3/20C11D3/50G01N27/30G01N27/49G01N33/49
CPCC11D3/50A61B5/1473G01N33/4925G01N27/3271C11D3/2093G01N27/3335C11D3/0015G01N27/416G01N27/48
Inventor 汤浅真阿部正彦山口有朋盐泽麻子石川满寿英江口胜哉木户茂
Owner 武林 敬
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com